PMC:7417788 / 62043-62905 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7417788","sourcedb":"PMC","sourceid":"7417788","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7417788","text":"PBMCs suspended in phosphate buffered saline (PBS) were cultured with Live/Dead yellow dye (Invitrogen) at 4 °C for 30 min and then washed once with 1 mL of PBS containing 1% FBS (GIBCO, Grand Island, NY, USA). Subsequently, cells were treated with antibodies for 30 min at 4 °C. These antibodies included: CD3-BV785 (clone SK7, Biolegend), CD19-APC (clone HB19, Biolegend), CD88-PE/Cy7 (clone S5/1, Biolegend), CD89-PE/Cy7 (clone A59, Biolegend), HLA-DR-FITC (clone L243, Biolegend), CD11c-BV421 (clone 3.9, Biolegend), FcεRIa- PercP/Cy5.5 (clone AER-37, Biolegend), CD1c-BV650 (clone L161, Biolegend), CD147-PE (clone HIM6, BD biosciences), CD371 (CLEC12A)-PE (clone 50C1, Biolegend). Analysis of PBMCs with flow cytometry was conducted with BD Fortessa (BD Biosciences) and the results were evaluated with FlowJo (version 10.0.7, Tree Star, Ashland, OR, USA).","tracks":[{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T1389","span":{"begin":87,"end":90},"obj":"CHEBI:37958;CHEBI:37958"},{"id":"T1390","span":{"begin":249,"end":259},"obj":"GO:0042571"},{"id":"T1391","span":{"begin":286,"end":296},"obj":"GO:0042571"},{"id":"T1392","span":{"begin":341,"end":345},"obj":"PR:000001002"},{"id":"T1393","span":{"begin":346,"end":349},"obj":"PR:000004122"},{"id":"T1394","span":{"begin":375,"end":379},"obj":"PR:000001173"},{"id":"T1395","span":{"begin":412,"end":416},"obj":"PR:000001857"},{"id":"T1396","span":{"begin":448,"end":451},"obj":"PR:000029150"},{"id":"T1397","span":{"begin":455,"end":459},"obj":"CHEBI:37926;CHEBI:37926"},{"id":"T1398","span":{"begin":485,"end":490},"obj":"PR:000001013"},{"id":"T1399","span":{"begin":535,"end":538},"obj":"CHEBI:37989;CHEBI:37989"},{"id":"T1400","span":{"begin":548,"end":551},"obj":"UBERON:0004356"},{"id":"T1401","span":{"begin":568,"end":572},"obj":"PR:000002027"},{"id":"T1402","span":{"begin":604,"end":609},"obj":"PR:000001324"}],"attributes":[{"subj":"T1389","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1390","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1391","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1392","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1393","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1394","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1395","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1396","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1397","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1398","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1399","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1400","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1401","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1402","pred":"source","obj":"LitCovid-PMC-OGER-BB"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T447","span":{"begin":225,"end":230},"obj":"Body_part"},{"id":"T448","span":{"begin":448,"end":451},"obj":"Body_part"}],"attributes":[{"id":"A447","pred":"fma_id","subj":"T447","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A448","pred":"fma_id","subj":"T448","obj":"http://purl.org/sig/ont/fma/fma84795"},{"subj":"T447","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T448","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T135","span":{"begin":626,"end":628},"obj":"Disease"},{"id":"T136","span":{"begin":744,"end":746},"obj":"Disease"},{"id":"T137","span":{"begin":757,"end":759},"obj":"Disease"}],"attributes":[{"id":"A135","pred":"mondo_id","subj":"T135","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"},{"id":"A136","pred":"mondo_id","subj":"T136","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"},{"id":"A137","pred":"mondo_id","subj":"T137","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"},{"subj":"T135","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T136","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T137","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1062","span":{"begin":107,"end":111},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T1063","span":{"begin":201,"end":203},"obj":"http://purl.obolibrary.org/obo/CLO_0009877"},{"id":"T1064","span":{"begin":225,"end":230},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1065","span":{"begin":274,"end":278},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T1066","span":{"begin":307,"end":310},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T1067","span":{"begin":307,"end":310},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"},{"id":"T1068","span":{"begin":341,"end":345},"obj":"http://purl.obolibrary.org/obo/CLO_0053432"},{"id":"T1069","span":{"begin":394,"end":396},"obj":"http://purl.obolibrary.org/obo/CLO_0008933"},{"id":"T1070","span":{"begin":467,"end":471},"obj":"http://purl.obolibrary.org/obo/CLO_0007180"},{"id":"T1071","span":{"begin":485,"end":490},"obj":"http://purl.obolibrary.org/obo/PR_000001013"},{"id":"T1072","span":{"begin":521,"end":523},"obj":"http://purl.obolibrary.org/obo/CLO_0052676"}],"attributes":[{"subj":"T1062","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1063","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1064","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1065","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1066","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1067","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1068","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1069","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1070","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1071","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1072","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T98657","span":{"begin":19,"end":28},"obj":"Chemical"},{"id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