PMC:7417788 / 62018-62905 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T1389","span":{"begin":112,"end":115},"obj":"CHEBI:37958;CHEBI:37958"},{"id":"T1390","span":{"begin":274,"end":284},"obj":"GO:0042571"},{"id":"T1391","span":{"begin":311,"end":321},"obj":"GO:0042571"},{"id":"T1392","span":{"begin":366,"end":370},"obj":"PR:000001002"},{"id":"T1393","span":{"begin":371,"end":374},"obj":"PR:000004122"},{"id":"T1394","span":{"begin":400,"end":404},"obj":"PR:000001173"},{"id":"T1395","span":{"begin":437,"end":441},"obj":"PR:000001857"},{"id":"T1396","span":{"begin":473,"end":476},"obj":"PR:000029150"},{"id":"T1397","span":{"begin":480,"end":484},"obj":"CHEBI:37926;CHEBI:37926"},{"id":"T1398","span":{"begin":510,"end":515},"obj":"PR:000001013"},{"id":"T1399","span":{"begin":560,"end":563},"obj":"CHEBI:37989;CHEBI:37989"},{"id":"T1400","span":{"begin":573,"end":576},"obj":"UBERON:0004356"},{"id":"T1401","span":{"begin":593,"end":597},"obj":"PR:000002027"},{"id":"T1402","span":{"begin":629,"end":634},"obj":"PR:000001324"}],"text":"Flow cytometric analysis\nPBMCs suspended in phosphate buffered saline (PBS) were cultured with Live/Dead yellow dye (Invitrogen) at 4 °C for 30 min and then washed once with 1 mL of PBS containing 1% FBS (GIBCO, Grand Island, NY, USA). Subsequently, cells were treated with antibodies for 30 min at 4 °C. These antibodies included: CD3-BV785 (clone SK7, Biolegend), CD19-APC (clone HB19, Biolegend), CD88-PE/Cy7 (clone S5/1, Biolegend), CD89-PE/Cy7 (clone A59, Biolegend), HLA-DR-FITC (clone L243, Biolegend), CD11c-BV421 (clone 3.9, Biolegend), FcεRIa- PercP/Cy5.5 (clone AER-37, Biolegend), CD1c-BV650 (clone L161, Biolegend), CD147-PE (clone HIM6, BD biosciences), CD371 (CLEC12A)-PE (clone 50C1, Biolegend). Analysis of PBMCs with flow cytometry was conducted with BD Fortessa (BD Biosciences) and the results were evaluated with FlowJo (version 10.0.7, Tree Star, Ashland, OR, USA)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T447","span":{"begin":250,"end":255},"obj":"Body_part"},{"id":"T448","span":{"begin":473,"end":476},"obj":"Body_part"}],"attributes":[{"id":"A447","pred":"fma_id","subj":"T447","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A448","pred":"fma_id","subj":"T448","obj":"http://purl.org/sig/ont/fma/fma84795"}],"text":"Flow cytometric analysis\nPBMCs suspended in phosphate buffered saline (PBS) were cultured with Live/Dead yellow dye (Invitrogen) at 4 °C for 30 min and then washed once with 1 mL of PBS containing 1% FBS (GIBCO, Grand Island, NY, USA). Subsequently, cells were treated with antibodies for 30 min at 4 °C. These antibodies included: CD3-BV785 (clone SK7, Biolegend), CD19-APC (clone HB19, Biolegend), CD88-PE/Cy7 (clone S5/1, Biolegend), CD89-PE/Cy7 (clone A59, Biolegend), HLA-DR-FITC (clone L243, Biolegend), CD11c-BV421 (clone 3.9, Biolegend), FcεRIa- PercP/Cy5.5 (clone AER-37, Biolegend), CD1c-BV650 (clone L161, Biolegend), CD147-PE (clone HIM6, BD biosciences), CD371 (CLEC12A)-PE (clone 50C1, Biolegend). Analysis of PBMCs with flow cytometry was conducted with BD Fortessa (BD Biosciences) and the results were evaluated with FlowJo (version 10.0.7, Tree Star, Ashland, OR, USA)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T135","span":{"begin":651,"end":653},"obj":"Disease"},{"id":"T136","span":{"begin":769,"end":771},"obj":"Disease"},{"id":"T137","span":{"begin":782,"end":784},"obj":"Disease"}],"attributes":[{"id":"A135","pred":"mondo_id","subj":"T135","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"},{"id":"A136","pred":"mondo_id","subj":"T136","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"},{"id":"A137","pred":"mondo_id","subj":"T137","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"}],"text":"Flow cytometric analysis\nPBMCs suspended in phosphate buffered saline (PBS) were cultured with Live/Dead yellow dye (Invitrogen) at 4 °C for 30 min and then washed once with 1 mL of PBS containing 1% FBS (GIBCO, Grand Island, NY, USA). Subsequently, cells were treated with antibodies for 30 min at 4 °C. These antibodies included: CD3-BV785 (clone SK7, Biolegend), CD19-APC (clone HB19, Biolegend), CD88-PE/Cy7 (clone S5/1, Biolegend), CD89-PE/Cy7 (clone A59, Biolegend), HLA-DR-FITC (clone L243, Biolegend), CD11c-BV421 (clone 3.9, Biolegend), FcεRIa- PercP/Cy5.5 (clone AER-37, Biolegend), CD1c-BV650 (clone L161, Biolegend), CD147-PE (clone HIM6, BD biosciences), CD371 (CLEC12A)-PE (clone 50C1, Biolegend). Analysis of PBMCs with flow cytometry was conducted with BD Fortessa (BD Biosciences) and the results were evaluated with FlowJo (version 10.0.7, Tree Star, Ashland, OR, USA)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1062","span":{"begin":132,"end":136},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T1063","span":{"begin":226,"end":228},"obj":"http://purl.obolibrary.org/obo/CLO_0009877"},{"id":"T1064","span":{"begin":250,"end":255},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1065","span":{"begin":299,"end":303},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T1066","span":{"begin":332,"end":335},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T1067","span":{"begin":332,"end":335},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"},{"id":"T1068","span":{"begin":366,"end":370},"obj":"http://purl.obolibrary.org/obo/CLO_0053432"},{"id":"T1069","span":{"begin":419,"end":421},"obj":"http://purl.obolibrary.org/obo/CLO_0008933"},{"id":"T1070","span":{"begin":492,"end":496},"obj":"http://purl.obolibrary.org/obo/CLO_0007180"},{"id":"T1071","span":{"begin":510,"end":515},"obj":"http://purl.obolibrary.org/obo/PR_000001013"},{"id":"T1072","span":{"begin":546,"end":548},"obj":"http://purl.obolibrary.org/obo/CLO_0052676"}],"text":"Flow cytometric analysis\nPBMCs suspended in phosphate buffered saline (PBS) were cultured with Live/Dead yellow dye (Invitrogen) at 4 °C for 30 min and then washed once with 1 mL of PBS containing 1% FBS (GIBCO, Grand Island, NY, USA). Subsequently, cells were treated with antibodies for 30 min at 4 °C. These antibodies included: CD3-BV785 (clone SK7, Biolegend), CD19-APC (clone HB19, Biolegend), CD88-PE/Cy7 (clone S5/1, Biolegend), CD89-PE/Cy7 (clone A59, Biolegend), HLA-DR-FITC (clone L243, Biolegend), CD11c-BV421 (clone 3.9, Biolegend), FcεRIa- PercP/Cy5.5 (clone AER-37, Biolegend), CD1c-BV650 (clone L161, Biolegend), CD147-PE (clone HIM6, BD biosciences), CD371 (CLEC12A)-PE (clone 50C1, Biolegend). Analysis of PBMCs with flow cytometry was conducted with BD Fortessa (BD Biosciences) and the results were evaluated with FlowJo (version 10.0.7, Tree Star, Ashland, OR, USA)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T98657","span":{"begin":44,"end":53},"obj":"Chemical"},{"id":"T60012","span":{"begin":112,"end":115},"obj":"Chemical"},{"id":"T5861","span":{"begin":226,"end":228},"obj":"Chemical"},{"id":"T75437","span":{"begin":371,"end":374},"obj":"Chemical"},{"id":"T20098","span":{"begin":405,"end":407},"obj":"Chemical"},{"id":"T92","span":{"begin":419,"end":421},"obj":"Chemical"},{"id":"T95897","span":{"begin":442,"end":444},"obj":"Chemical"},{"id":"T65030","span":{"begin":477,"end":479},"obj":"Chemical"},{"id":"T97","span":{"begin":480,"end":484},"obj":"Chemical"},{"id":"T20259","span":{"begin":635,"end":637},"obj":"Chemical"},{"id":"T74014","span":{"begin":684,"end":686},"obj":"Chemical"}],"attributes":[{"id":"A81773","pred":"chebi_id","subj":"T98657","obj":"http://purl.obolibrary.org/obo/CHEBI_18367"},{"id":"A77236","pred":"chebi_id","subj":"T98657","obj":"http://purl.obolibrary.org/obo/CHEBI_26020"},{"id":"A10433","pred":"chebi_id","subj":"T98657","obj":"http://purl.obolibrary.org/obo/CHEBI_35780"},{"id":"A85340","pred":"chebi_id","subj":"T98657","obj":"http://purl.obolibrary.org/obo/CHEBI_43474"},{"id":"A42258","pred":"chebi_id","subj":"T60012","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A84438","pred":"chebi_id","subj":"T5861","obj":"http://purl.obolibrary.org/obo/CHEBI_73427"},{"id":"A43456","pred":"chebi_id","subj":"T75437","obj":"http://purl.obolibrary.org/obo/CHEBI_80551"},{"id":"A36065","pred":"chebi_id","subj":"T20098","obj":"http://purl.obolibrary.org/obo/CHEBI_16038"},{"id":"A40574","pred":"chebi_id","subj":"T20098","obj":"http://purl.obolibrary.org/obo/CHEBI_17553"},{"id":"A39089","pred":"chebi_id","subj":"T20098","obj":"http://purl.obolibrary.org/obo/CHEBI_74762"},{"id":"A90763","pred":"chebi_id","subj":"T92","obj":"http://purl.obolibrary.org/obo/CHEBI_29386"},{"id":"A38300","pred":"chebi_id","subj":"T95897","obj":"http://purl.obolibrary.org/obo/CHEBI_16038"},{"id":"A53491","pred":"chebi_id","subj":"T95897","obj":"http://purl.obolibrary.org/obo/CHEBI_17553"},{"id":"A52230","pred":"chebi_id","subj":"T95897","obj":"http://purl.obolibrary.org/obo/CHEBI_74762"},{"id":"A42107","pred":"chebi_id","subj":"T65030","obj":"http://purl.obolibrary.org/obo/CHEBI_73445"},{"id":"A43246","pred":"chebi_id","subj":"T97","obj":"http://purl.obolibrary.org/obo/CHEBI_37926"},{"id":"A79265","pred":"chebi_id","subj":"T20259","obj":"http://purl.obolibrary.org/obo/CHEBI_16038"},{"id":"A92523","pred":"chebi_id","subj":"T20259","obj":"http://purl.obolibrary.org/obo/CHEBI_17553"},{"id":"A66788","pred":"chebi_id","subj":"T20259","obj":"http://purl.obolibrary.org/obo/CHEBI_74762"},{"id":"A20735","pred":"chebi_id","subj":"T74014","obj":"http://purl.obolibrary.org/obo/CHEBI_16038"},{"id":"A92376","pred":"chebi_id","subj":"T74014","obj":"http://purl.obolibrary.org/obo/CHEBI_17553"},{"id":"A76502","pred":"chebi_id","subj":"T74014","obj":"http://purl.obolibrary.org/obo/CHEBI_74762"}],"text":"Flow cytometric analysis\nPBMCs suspended in phosphate buffered saline (PBS) were cultured with Live/Dead yellow dye (Invitrogen) at 4 °C for 30 min and then washed once with 1 mL of PBS containing 1% FBS (GIBCO, Grand Island, NY, USA). Subsequently, cells were treated with antibodies for 30 min at 4 °C. These antibodies included: CD3-BV785 (clone SK7, Biolegend), CD19-APC (clone HB19, Biolegend), CD88-PE/Cy7 (clone S5/1, Biolegend), CD89-PE/Cy7 (clone A59, Biolegend), HLA-DR-FITC (clone L243, Biolegend), CD11c-BV421 (clone 3.9, Biolegend), FcεRIa- PercP/Cy5.5 (clone AER-37, Biolegend), CD1c-BV650 (clone L161, Biolegend), CD147-PE (clone HIM6, BD biosciences), CD371 (CLEC12A)-PE (clone 50C1, Biolegend). Analysis of PBMCs with flow cytometry was conducted with BD Fortessa (BD Biosciences) and the results were evaluated with FlowJo (version 10.0.7, Tree Star, Ashland, OR, USA)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T342","span":{"begin":0,"end":24},"obj":"Sentence"},{"id":"T343","span":{"begin":25,"end":235},"obj":"Sentence"},{"id":"T344","span":{"begin":236,"end":304},"obj":"Sentence"},{"id":"T345","span":{"begin":305,"end":711},"obj":"Sentence"},{"id":"T346","span":{"begin":712,"end":887},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Flow cytometric analysis\nPBMCs suspended in phosphate buffered saline (PBS) were cultured with Live/Dead yellow dye (Invitrogen) at 4 °C for 30 min and then washed once with 1 mL of PBS containing 1% FBS (GIBCO, Grand Island, NY, USA). Subsequently, cells were treated with antibodies for 30 min at 4 °C. These antibodies included: CD3-BV785 (clone SK7, Biolegend), CD19-APC (clone HB19, Biolegend), CD88-PE/Cy7 (clone S5/1, Biolegend), CD89-PE/Cy7 (clone A59, Biolegend), HLA-DR-FITC (clone L243, Biolegend), CD11c-BV421 (clone 3.9, Biolegend), FcεRIa- PercP/Cy5.5 (clone AER-37, Biolegend), CD1c-BV650 (clone L161, Biolegend), CD147-PE (clone HIM6, BD biosciences), CD371 (CLEC12A)-PE (clone 50C1, Biolegend). Analysis of PBMCs with flow cytometry was conducted with BD Fortessa (BD Biosciences) and the results were evaluated with FlowJo (version 10.0.7, Tree Star, Ashland, OR, USA)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1395","span":{"begin":366,"end":370},"obj":"Gene"},{"id":"1396","span":{"begin":400,"end":404},"obj":"Gene"},{"id":"1397","span":{"begin":437,"end":441},"obj":"Gene"},{"id":"1398","span":{"begin":510,"end":515},"obj":"Gene"},{"id":"1399","span":{"begin":593,"end":597},"obj":"Gene"},{"id":"1400","span":{"begin":629,"end":634},"obj":"Gene"},{"id":"1401","span":{"begin":668,"end":673},"obj":"Gene"},{"id":"1402","span":{"begin":675,"end":682},"obj":"Gene"},{"id":"1403","span":{"begin":44,"end":69},"obj":"Chemical"},{"id":"1404","span":{"begin":71,"end":74},"obj":"Chemical"},{"id":"1405","span":{"begin":182,"end":185},"obj":"Chemical"},{"id":"1406","span":{"begin":200,"end":203},"obj":"Disease"},{"id":"1407","span":{"begin":371,"end":374},"obj":"Disease"},{"id":"1408","span":{"begin":546,"end":552},"obj":"Disease"},{"id":"1409","span":{"begin":336,"end":341},"obj":"CellLine"},{"id":"1410","span":{"begin":349,"end":352},"obj":"CellLine"},{"id":"1411","span":{"begin":516,"end":521},"obj":"CellLine"}],"attributes":[{"id":"A1395","pred":"tao:has_database_id","subj":"1395","obj":"Gene:930"},{"id":"A1396","pred":"tao:has_database_id","subj":"1396","obj":"Gene:728"},{"id":"A1397","pred":"tao:has_database_id","subj":"1397","obj":"Gene:2204"},{"id":"A1398","pred":"tao:has_database_id","subj":"1398","obj":"Gene:3687"},{"id":"A1399","pred":"tao:has_database_id","subj":"1399","obj":"Gene:911"},{"id":"A1400","pred":"tao:has_database_id","subj":"1400","obj":"Gene:682"},{"id":"A1401","pred":"tao:has_database_id","subj":"1401","obj":"Gene:160364"},{"id":"A1402","pred":"tao:has_database_id","subj":"1402","obj":"Gene:160364"},{"id":"A1406","pred":"tao:has_database_id","subj":"1406","obj":"MESH:D005198"},{"id":"A1407","pred":"tao:has_database_id","subj":"1407","obj":"MESH:D011125"},{"id":"A1409","pred":"tao:has_database_id","subj":"1409","obj":"CVCL:N487"},{"id":"A1410","pred":"tao:has_database_id","subj":"1410","obj":"CVCL:L697"},{"id":"A1411","pred":"tao:has_database_id","subj":"1411","obj":"CVCL:0182"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Flow cytometric analysis\nPBMCs suspended in phosphate buffered saline (PBS) were cultured with Live/Dead yellow dye (Invitrogen) at 4 °C for 30 min and then washed once with 1 mL of PBS containing 1% FBS (GIBCO, Grand Island, NY, USA). Subsequently, cells were treated with antibodies for 30 min at 4 °C. These antibodies included: CD3-BV785 (clone SK7, Biolegend), CD19-APC (clone HB19, Biolegend), CD88-PE/Cy7 (clone S5/1, Biolegend), CD89-PE/Cy7 (clone A59, Biolegend), HLA-DR-FITC (clone L243, Biolegend), CD11c-BV421 (clone 3.9, Biolegend), FcεRIa- PercP/Cy5.5 (clone AER-37, Biolegend), CD1c-BV650 (clone L161, Biolegend), CD147-PE (clone HIM6, BD biosciences), CD371 (CLEC12A)-PE (clone 50C1, Biolegend). Analysis of PBMCs with flow cytometry was conducted with BD Fortessa (BD Biosciences) and the results were evaluated with FlowJo (version 10.0.7, Tree Star, Ashland, OR, USA)."}