Id |
Subject |
Object |
Predicate |
Lexical cue |
T452 |
0-16 |
Sentence |
denotes |
Batch correction |
T453 |
17-105 |
Sentence |
denotes |
During the sample acquisition period, the flow panel was changed to remove one antibody. |
T454 |
106-237 |
Sentence |
denotes |
Batch correction was performed for samples acquired before and after this change to remove potential bias from downstream analysis. |
T455 |
238-512 |
Sentence |
denotes |
Because the primary flow features were expressed as a fraction of the parent population (falling in the 0-to-1 interval) a variance stabilizing transform (logit) was first applied to each data value, prior to re-centering the second panel to have the same mean as the first. |
T456 |
513-624 |
Sentence |
denotes |
After mean-centering, data were transformed back to the original fraction of parent scale by inverse transform. |
T457 |
625-737 |
Sentence |
denotes |
This procedure was applied separately to all 553 flow features annotated in the main text and supplemental data. |
T458 |
738-862 |
Sentence |
denotes |
Notably, this procedure avoids any batch-corrected feature values artificially falling outside of the original 0 to 1 range. |
T459 |
863-1016 |
Sentence |
denotes |
Following batch correction, neither UMAP Component 1 nor Component 2 had a statistically significant difference between panels by unpaired Wilcoxon test. |