PMC:7402624 / 67844-68326 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T533","span":{"begin":128,"end":133},"obj":"Body_part"}],"attributes":[{"id":"A533","pred":"fma_id","subj":"T533","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}

{"project":"LitCovid-PubTator","denotations":[{"id":"2082","span":{"begin":246,"end":250},"obj":"Gene"},{"id":"2083","span":{"begin":251,"end":256},"obj":"Gene"},{"id":"2084","span":{"begin":477,"end":481},"obj":"Gene"},{"id":"2085","span":{"begin":240,"end":245},"obj":"Species"},{"id":"2086","span":{"begin":92,"end":95},"obj":"Chemical"},{"id":"2087","span":{"begin":319,"end":323},"obj":"CellLine"}],"attributes":[{"id":"A2082","pred":"tao:has_database_id","subj":"2082","obj":"Gene:940"},{"id":"A2083","pred":"tao:has_database_id","subj":"2083","obj":"Gene:3676"},{"id":"A2084","pred":"tao:has_database_id","subj":"2084","obj":"Gene:3458"},{"id":"A2085","pred":"tao:has_database_id","subj":"2085","obj":"Tax:9606"},{"id":"A2086","pred":"tao:has_database_id","subj":"2086","obj":"MESH:D007854"},{"id":"A2087","pred":"tao:has_database_id","subj":"2087","obj":"CVCL:M775"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T433","span":{"begin":212,"end":214},"obj":"Disease"},{"id":"T434","span":{"begin":333,"end":335},"obj":"Disease"}],"attributes":[{"id":"A433","pred":"mondo_id","subj":"T433","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"},{"id":"A434","pred":"mondo_id","subj":"T434","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"}],"text":"96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T984","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T985","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"},{"id":"T986","span":{"begin":99,"end":102},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T987","span":{"begin":128,"end":133},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T988","span":{"begin":160,"end":170},"obj":"http://purl.obolibrary.org/obo/CLO_0001040"},{"id":"T989","span":{"begin":240,"end":245},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T990","span":{"begin":308,"end":311},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T991","span":{"begin":308,"end":311},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"}],"text":"96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}

{"project":"LitCovid-sentences","denotations":[{"id":"T416","span":{"begin":0,"end":113},"obj":"Sentence"},{"id":"T417","span":{"begin":114,"end":224},"obj":"Sentence"},{"id":"T418","span":{"begin":225,"end":337},"obj":"Sentence"},{"id":"T419","span":{"begin":338,"end":482},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}