PMC:7402624 / 67797-68326 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T533","span":{"begin":175,"end":180},"obj":"Body_part"}],"attributes":[{"id":"A533","pred":"fma_id","subj":"T533","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Intracellular stain after CD3/CD28 stimulation\n96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}

{"project":"LitCovid-PubTator","denotations":[{"id":"2075","span":{"begin":30,"end":34},"obj":"Gene"},{"id":"2082","span":{"begin":293,"end":297},"obj":"Gene"},{"id":"2083","span":{"begin":298,"end":303},"obj":"Gene"},{"id":"2084","span":{"begin":524,"end":528},"obj":"Gene"},{"id":"2085","span":{"begin":287,"end":292},"obj":"Species"},{"id":"2086","span":{"begin":139,"end":142},"obj":"Chemical"},{"id":"2087","span":{"begin":366,"end":370},"obj":"CellLine"}],"attributes":[{"id":"A2075","pred":"tao:has_database_id","subj":"2075","obj":"Gene:940"},{"id":"A2082","pred":"tao:has_database_id","subj":"2082","obj":"Gene:940"},{"id":"A2083","pred":"tao:has_database_id","subj":"2083","obj":"Gene:3676"},{"id":"A2084","pred":"tao:has_database_id","subj":"2084","obj":"Gene:3458"},{"id":"A2085","pred":"tao:has_database_id","subj":"2085","obj":"Tax:9606"},{"id":"A2086","pred":"tao:has_database_id","subj":"2086","obj":"MESH:D007854"},{"id":"A2087","pred":"tao:has_database_id","subj":"2087","obj":"CVCL:M775"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Intracellular stain after CD3/CD28 stimulation\n96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T433","span":{"begin":259,"end":261},"obj":"Disease"},{"id":"T434","span":{"begin":380,"end":382},"obj":"Disease"}],"attributes":[{"id":"A433","pred":"mondo_id","subj":"T433","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"},{"id":"A434","pred":"mondo_id","subj":"T434","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"}],"text":"Intracellular stain after CD3/CD28 stimulation\n96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T982","span":{"begin":26,"end":29},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T983","span":{"begin":26,"end":29},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"},{"id":"T984","span":{"begin":106,"end":109},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T985","span":{"begin":106,"end":109},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"},{"id":"T986","span":{"begin":146,"end":149},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T987","span":{"begin":175,"end":180},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T988","span":{"begin":207,"end":217},"obj":"http://purl.obolibrary.org/obo/CLO_0001040"},{"id":"T989","span":{"begin":287,"end":292},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T990","span":{"begin":355,"end":358},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T991","span":{"begin":355,"end":358},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"}],"text":"Intracellular stain after CD3/CD28 stimulation\n96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}

{"project":"LitCovid-sentences","denotations":[{"id":"T415","span":{"begin":0,"end":46},"obj":"Sentence"},{"id":"T416","span":{"begin":47,"end":160},"obj":"Sentence"},{"id":"T417","span":{"begin":161,"end":271},"obj":"Sentence"},{"id":"T418","span":{"begin":272,"end":384},"obj":"Sentence"},{"id":"T419","span":{"begin":385,"end":529},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Intracellular stain after CD3/CD28 stimulation\n96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ."}