PMC:7402624 / 67797-68326
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T533 | 175-180 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 2075 | 30-34 | Gene | denotes | CD28 | Gene:940 |
| 2082 | 293-297 | Gene | denotes | CD28 | Gene:940 |
| 2083 | 298-303 | Gene | denotes | CD49d | Gene:3676 |
| 2084 | 524-528 | Gene | denotes | IFNɣ | Gene:3458 |
| 2085 | 287-292 | Species | denotes | human | Tax:9606 |
| 2086 | 139-142 | Chemical | denotes | PBS | MESH:D007854 |
| 2087 | 366-370 | CellLine | denotes | L293 | CVCL:M775 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T433 | 259-261 | Disease | denotes | BD | http://purl.obolibrary.org/obo/MONDO_0007191 |
| T434 | 380-382 | Disease | denotes | BD | http://purl.obolibrary.org/obo/MONDO_0007191 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T982 | 26-29 | http://purl.obolibrary.org/obo/CLO_0052882 | denotes | CD3 |
| T983 | 26-29 | http://purl.obolibrary.org/obo/CLO_0053434 | denotes | CD3 |
| T984 | 106-109 | http://purl.obolibrary.org/obo/CLO_0052882 | denotes | CD3 |
| T985 | 106-109 | http://purl.obolibrary.org/obo/CLO_0053434 | denotes | CD3 |
| T986 | 146-149 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4°C |
| T987 | 175-180 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T988 | 207-217 | http://purl.obolibrary.org/obo/CLO_0001040 | denotes | at 1 × 105 |
| T989 | 287-292 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | human |
| T990 | 355-358 | http://purl.obolibrary.org/obo/CLO_0052882 | denotes | CD3 |
| T991 | 355-358 | http://purl.obolibrary.org/obo/CLO_0053434 | denotes | CD3 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T415 | 0-46 | Sentence | denotes | Intracellular stain after CD3/CD28 stimulation |
| T416 | 47-160 | Sentence | denotes | 96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. |
| T417 | 161-271 | Sentence | denotes | The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). |
| T418 | 272-384 | Sentence | denotes | 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). |
| T419 | 385-529 | Sentence | denotes | GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ. |