PMC:7376845 / 8938-9946 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T61","span":{"begin":44,"end":49},"obj":"Body_part"},{"id":"T62","span":{"begin":291,"end":296},"obj":"Body_part"},{"id":"T63","span":{"begin":361,"end":366},"obj":"Body_part"},{"id":"T64","span":{"begin":493,"end":496},"obj":"Body_part"},{"id":"T65","span":{"begin":497,"end":505},"obj":"Body_part"},{"id":"T66","span":{"begin":867,"end":871},"obj":"Body_part"}],"attributes":[{"id":"A61","pred":"fma_id","subj":"T61","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A62","pred":"fma_id","subj":"T62","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A63","pred":"fma_id","subj":"T63","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A64","pred":"fma_id","subj":"T64","obj":"http://purl.org/sig/ont/fma/fma62872"},{"id":"A65","pred":"fma_id","subj":"T65","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A66","pred":"fma_id","subj":"T66","obj":"http://purl.org/sig/ont/fma/fma58241"}],"text":"For immunofluorescence (IF) analysis, COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at RT followed by permeabilisation with 0.2% Triton X-100 (Sigma-Aldrich, St. Louis, MO, United States) for 5 min. Fixed cells were then blocked with PBS containing 10% FBS for 30 min at RT. Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies, Carlsbad, CA, United States). Immunolabelled coverslips were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and mounted using ProLong Gold Antifade Mountant (Molecular Probes, Eugene, OR, United States). Images were acquired with Olympus CKX53 microscope using Olympus (Tokyo, Japan) LCAch N 20×/0.40 iPC objective lens and Olympus DP27 colour camera with Olympus cellSens software. Each channel was collected separately, with images at 1024 × 1024 pixels."}

{"project":"LitCovid-PubTator","denotations":[{"id":"256","span":{"begin":423,"end":429},"obj":"Species"},{"id":"257","span":{"begin":142,"end":158},"obj":"Chemical"},{"id":"258","span":{"begin":215,"end":227},"obj":"Chemical"},{"id":"259","span":{"begin":320,"end":323},"obj":"Chemical"},{"id":"260","span":{"begin":450,"end":465},"obj":"Chemical"},{"id":"261","span":{"begin":607,"end":636},"obj":"Chemical"},{"id":"262","span":{"begin":638,"end":642},"obj":"Chemical"},{"id":"263","span":{"begin":38,"end":43},"obj":"CellLine"}],"attributes":[{"id":"A256","pred":"tao:has_database_id","subj":"256","obj":"Tax:10090"},{"id":"A257","pred":"tao:has_database_id","subj":"257","obj":"MESH:C003043"},{"id":"A258","pred":"tao:has_database_id","subj":"258","obj":"MESH:D017830"},{"id":"A261","pred":"tao:has_database_id","subj":"261","obj":"MESH:C007293"},{"id":"A262","pred":"tao:has_database_id","subj":"262","obj":"MESH:C007293"},{"id":"A263","pred":"tao:has_database_id","subj":"263","obj":"CVCL:0224"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"For immunofluorescence (IF) analysis, COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at RT followed by permeabilisation with 0.2% Triton X-100 (Sigma-Aldrich, St. Louis, MO, United States) for 5 min. Fixed cells were then blocked with PBS containing 10% FBS for 30 min at RT. Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies, Carlsbad, CA, United States). Immunolabelled coverslips were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and mounted using ProLong Gold Antifade Mountant (Molecular Probes, Eugene, OR, United States). Images were acquired with Olympus CKX53 microscope using Olympus (Tokyo, Japan) LCAch N 20×/0.40 iPC objective lens and Olympus DP27 colour camera with Olympus cellSens software. Each channel was collected separately, with images at 1024 × 1024 pixels."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T108","span":{"begin":38,"end":49},"obj":"http://purl.obolibrary.org/obo/CLO_0051656"},{"id":"T109","span":{"begin":38,"end":49},"obj":"http://purl.obolibrary.org/obo/CLO_0051657"},{"id":"T110","span":{"begin":38,"end":49},"obj":"http://purl.obolibrary.org/obo/CLO_0051658"},{"id":"T111","span":{"begin":38,"end":43},"obj":"http://purl.obolibrary.org/obo/CLO_0002597"},{"id":"T112","span":{"begin":38,"end":43},"obj":"http://purl.obolibrary.org/obo/CLO_0050508"},{"id":"T113","span":{"begin":244,"end":246},"obj":"http://purl.obolibrary.org/obo/CLO_0009141"},{"id":"T114","span":{"begin":244,"end":246},"obj":"http://purl.obolibrary.org/obo/CLO_0050980"},{"id":"T115","span":{"begin":255,"end":257},"obj":"http://purl.obolibrary.org/obo/CLO_0007815"},{"id":"T116","span":{"begin":291,"end":296},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T117","span":{"begin":361,"end":366},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T118","span":{"begin":438,"end":440},"obj":"http://purl.obolibrary.org/obo/CLO_0053799"},{"id":"T119","span":{"begin":477,"end":481},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T120","span":{"begin":487,"end":492},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"},{"id":"T121","span":{"begin":853,"end":856},"obj":"http://purl.obolibrary.org/obo/CL_0000784"},{"id":"T122","span":{"begin":857,"end":866},"obj":"http://purl.obolibrary.org/obo/BFO_0000030"},{"id":"T123","span":{"begin":867,"end":871},"obj":"http://purl.obolibrary.org/obo/UBERON_0005389"}],"text":"For immunofluorescence (IF) analysis, COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at RT followed by permeabilisation with 0.2% Triton X-100 (Sigma-Aldrich, St. Louis, MO, United States) for 5 min. Fixed cells were then blocked with PBS containing 10% FBS for 30 min at RT. Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies, Carlsbad, CA, United States). Immunolabelled coverslips were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and mounted using ProLong Gold Antifade Mountant (Molecular Probes, Eugene, OR, United States). Images were acquired with Olympus CKX53 microscope using Olympus (Tokyo, Japan) LCAch N 20×/0.40 iPC objective lens and Olympus DP27 colour camera with Olympus cellSens software. Each channel was collected separately, with images at 1024 × 1024 pixels."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T93","span":{"begin":215,"end":227},"obj":"Chemical"},{"id":"T94","span":{"begin":450,"end":465},"obj":"Chemical"},{"id":"T95","span":{"begin":456,"end":461},"obj":"Chemical"},{"id":"T96","span":{"begin":624,"end":636},"obj":"Chemical"},{"id":"T97","span":{"begin":638,"end":642},"obj":"Chemical"},{"id":"T98","span":{"begin":686,"end":690},"obj":"Chemical"}],"attributes":[{"id":"A93","pred":"chebi_id","subj":"T93","obj":"http://purl.obolibrary.org/obo/CHEBI_9750"},{"id":"A94","pred":"chebi_id","subj":"T94","obj":"http://purl.obolibrary.org/obo/CHEBI_52661"},{"id":"A95","pred":"chebi_id","subj":"T95","obj":"http://purl.obolibrary.org/obo/CHEBI_24061"},{"id":"A96","pred":"chebi_id","subj":"T96","obj":"http://purl.obolibrary.org/obo/CHEBI_48559"},{"id":"A97","pred":"chebi_id","subj":"T97","obj":"http://purl.obolibrary.org/obo/CHEBI_51231"},{"id":"A98","pred":"chebi_id","subj":"T98","obj":"http://purl.obolibrary.org/obo/CHEBI_29287"}],"text":"For immunofluorescence (IF) analysis, COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at RT followed by permeabilisation with 0.2% Triton X-100 (Sigma-Aldrich, St. Louis, MO, United States) for 5 min. Fixed cells were then blocked with PBS containing 10% FBS for 30 min at RT. Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies, Carlsbad, CA, United States). Immunolabelled coverslips were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and mounted using ProLong Gold Antifade Mountant (Molecular Probes, Eugene, OR, United States). Images were acquired with Olympus CKX53 microscope using Olympus (Tokyo, Japan) LCAch N 20×/0.40 iPC objective lens and Olympus DP27 colour camera with Olympus cellSens software. Each channel was collected separately, with images at 1024 × 1024 pixels."}

{"project":"LitCovid-sentences","denotations":[{"id":"T65","span":{"begin":0,"end":284},"obj":"Sentence"},{"id":"T66","span":{"begin":285,"end":360},"obj":"Sentence"},{"id":"T67","span":{"begin":361,"end":555},"obj":"Sentence"},{"id":"T68","span":{"begin":556,"end":755},"obj":"Sentence"},{"id":"T69","span":{"begin":756,"end":934},"obj":"Sentence"},{"id":"T70","span":{"begin":935,"end":1008},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"For immunofluorescence (IF) analysis, COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at RT followed by permeabilisation with 0.2% Triton X-100 (Sigma-Aldrich, St. Louis, MO, United States) for 5 min. Fixed cells were then blocked with PBS containing 10% FBS for 30 min at RT. Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies, Carlsbad, CA, United States). Immunolabelled coverslips were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and mounted using ProLong Gold Antifade Mountant (Molecular Probes, Eugene, OR, United States). Images were acquired with Olympus CKX53 microscope using Olympus (Tokyo, Japan) LCAch N 20×/0.40 iPC objective lens and Olympus DP27 colour camera with Olympus cellSens software. Each channel was collected separately, with images at 1024 × 1024 pixels."}