PMC:7376845 / 6350-6856 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T42","span":{"begin":27,"end":35},"obj":"Body_part"},{"id":"T43","span":{"begin":125,"end":129},"obj":"Body_part"},{"id":"T44","span":{"begin":164,"end":171},"obj":"Body_part"},{"id":"T45","span":{"begin":457,"end":464},"obj":"Body_part"}],"attributes":[{"id":"A42","pred":"fma_id","subj":"T42","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A43","pred":"fma_id","subj":"T43","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A44","pred":"fma_id","subj":"T44","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A45","pred":"fma_id","subj":"T45","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"Purification of monoclonal antibody 1A9\nThe hybridoma for mAb 1A9 was previously generated [20]. All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"200","span":{"begin":299,"end":322},"obj":"Chemical"},{"id":"201","span":{"begin":323,"end":337},"obj":"Chemical"},{"id":"202","span":{"begin":359,"end":362},"obj":"Chemical"},{"id":"203","span":{"begin":442,"end":456},"obj":"Disease"}],"attributes":[{"id":"A200","pred":"tao:has_database_id","subj":"200","obj":"MESH:D012967"},{"id":"A201","pred":"tao:has_database_id","subj":"201","obj":"MESH:C016679"},{"id":"A202","pred":"tao:has_database_id","subj":"202","obj":"MESH:D012967"},{"id":"A203","pred":"tao:has_database_id","subj":"203","obj":"MESH:D007625"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Purification of monoclonal antibody 1A9\nThe hybridoma for mAb 1A9 was previously generated [20]. All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T84","span":{"begin":44,"end":53},"obj":"http://purl.obolibrary.org/obo/CLO_0036932"},{"id":"T85","span":{"begin":125,"end":129},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T86","span":{"begin":174,"end":176},"obj":"http://purl.obolibrary.org/obo/CLO_0003797"},{"id":"T87","span":{"begin":174,"end":176},"obj":"http://purl.obolibrary.org/obo/PR_000008725"}],"text":"Purification of monoclonal antibody 1A9\nThe hybridoma for mAb 1A9 was previously generated [20]. All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T60","span":{"begin":164,"end":171},"obj":"Chemical"},{"id":"T61","span":{"begin":174,"end":176},"obj":"Chemical"},{"id":"T62","span":{"begin":195,"end":197},"obj":"Chemical"},{"id":"T63","span":{"begin":219,"end":221},"obj":"Chemical"},{"id":"T65","span":{"begin":299,"end":322},"obj":"Chemical"},{"id":"T66","span":{"begin":299,"end":305},"obj":"Chemical"},{"id":"T67","span":{"begin":306,"end":313},"obj":"Chemical"},{"id":"T68","span":{"begin":314,"end":322},"obj":"Chemical"},{"id":"T69","span":{"begin":323,"end":337},"obj":"Chemical"},{"id":"T72","span":{"begin":359,"end":362},"obj":"Chemical"},{"id":"T73","span":{"begin":457,"end":464},"obj":"Chemical"},{"id":"T74","span":{"begin":471,"end":478},"obj":"Chemical"}],"attributes":[{"id":"A60","pred":"chebi_id","subj":"T60","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A61","pred":"chebi_id","subj":"T61","obj":"http://purl.obolibrary.org/obo/CHEBI_74055"},{"id":"A62","pred":"chebi_id","subj":"T62","obj":"http://purl.obolibrary.org/obo/CHEBI_73801"},{"id":"A63","pred":"chebi_id","subj":"T63","obj":"http://purl.obolibrary.org/obo/CHEBI_63895"},{"id":"A64","pred":"chebi_id","subj":"T63","obj":"http://purl.obolibrary.org/obo/CHEBI_74072"},{"id":"A65","pred":"chebi_id","subj":"T65","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"},{"id":"A66","pred":"chebi_id","subj":"T66","obj":"http://purl.obolibrary.org/obo/CHEBI_26708"},{"id":"A67","pred":"chebi_id","subj":"T67","obj":"http://purl.obolibrary.org/obo/CHEBI_23870"},{"id":"A68","pred":"chebi_id","subj":"T68","obj":"http://purl.obolibrary.org/obo/CHEBI_16189"},{"id":"A69","pred":"chebi_id","subj":"T69","obj":"http://purl.obolibrary.org/obo/CHEBI_51135"},{"id":"A70","pred":"chebi_id","subj":"T69","obj":"http://purl.obolibrary.org/obo/CHEBI_53656"},{"id":"A71","pred":"chebi_id","subj":"T69","obj":"http://purl.obolibrary.org/obo/CHEBI_60766"},{"id":"A72","pred":"chebi_id","subj":"T72","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"},{"id":"A73","pred":"chebi_id","subj":"T73","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A74","pred":"chebi_id","subj":"T74","obj":"http://purl.obolibrary.org/obo/CHEBI_33893"}],"text":"Purification of monoclonal antibody 1A9\nThe hybridoma for mAb 1A9 was previously generated [20]. All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T46","span":{"begin":0,"end":39},"obj":"Sentence"},{"id":"T47","span":{"begin":40,"end":96},"obj":"Sentence"},{"id":"T48","span":{"begin":97,"end":259},"obj":"Sentence"},{"id":"T49","span":{"begin":260,"end":378},"obj":"Sentence"},{"id":"T50","span":{"begin":379,"end":506},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Purification of monoclonal antibody 1A9\nThe hybridoma for mAb 1A9 was previously generated [20]. All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific)."}

{"project":"2_test","denotations":[{"id":"32700671-16378996-29327476","span":{"begin":92,"end":94},"obj":"16378996"}],"text":"Purification of monoclonal antibody 1A9\nThe hybridoma for mAb 1A9 was previously generated [20]. All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific)."}

{"project":"MyTest","denotations":[{"id":"32700671-16378996-29327476","span":{"begin":92,"end":94},"obj":"16378996"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"Purification of monoclonal antibody 1A9\nThe hybridoma for mAb 1A9 was previously generated [20]. All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific)."}