PMC:7376845 / 19390-20321 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T120","span":{"begin":253,"end":260},"obj":"Body_part"},{"id":"T121","span":{"begin":284,"end":289},"obj":"Body_part"},{"id":"T122","span":{"begin":578,"end":583},"obj":"Body_part"},{"id":"T123","span":{"begin":591,"end":596},"obj":"Body_part"},{"id":"T124","span":{"begin":684,"end":689},"obj":"Body_part"},{"id":"T125","span":{"begin":732,"end":739},"obj":"Body_part"},{"id":"T126","span":{"begin":834,"end":841},"obj":"Body_part"}],"attributes":[{"id":"A120","pred":"fma_id","subj":"T120","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A121","pred":"fma_id","subj":"T121","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A122","pred":"fma_id","subj":"T122","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A123","pred":"fma_id","subj":"T123","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A124","pred":"fma_id","subj":"T124","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A125","pred":"fma_id","subj":"T125","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A126","pred":"fma_id","subj":"T126","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"Four mAbs with distinct binding profiles to SARS-CoV-1, as previously mapped by internal deletion mutagenesis study, were selected for testing to determine if they cross-react with SARS-CoV-2. A fragment containing residues 1048 to 1206 of SARS-CoV-2 S protein was expressed in 293FT cells via transient transfection and WB analysis was performed using the four mAbs, namely 2B2, 1A9, 4B12 and 1G10. As shown in Figure 2A, all four mAbs detected this fragment of SARS-CoV-2, which is consistent with the sequence alignment shown in Figure 1. Due to the easy detachment of 293FT cells, COS-7 cells were used for IF assay instead. IF analysis performed on transiently transfected COS-7 cells showed binding of the four mAbs to this S protein fragment of SARS-CoV-2 (Figure 2B). These interactions are also specific for the SARS-COV-2 S protein (1048–1206) fragment as all four mAbs did not show binding to the negative control HBcAg."}

{"project":"LitCovid-PubTator","denotations":[{"id":"486","span":{"begin":44,"end":52},"obj":"Species"},{"id":"487","span":{"begin":181,"end":191},"obj":"Species"},{"id":"488","span":{"begin":240,"end":250},"obj":"Species"},{"id":"489","span":{"begin":463,"end":473},"obj":"Species"},{"id":"490","span":{"begin":752,"end":762},"obj":"Species"},{"id":"491","span":{"begin":821,"end":831},"obj":"Species"},{"id":"492","span":{"begin":278,"end":283},"obj":"CellLine"},{"id":"493","span":{"begin":572,"end":577},"obj":"CellLine"},{"id":"494","span":{"begin":585,"end":590},"obj":"CellLine"},{"id":"495","span":{"begin":678,"end":683},"obj":"CellLine"}],"attributes":[{"id":"A486","pred":"tao:has_database_id","subj":"486","obj":"Tax:694009"},{"id":"A487","pred":"tao:has_database_id","subj":"487","obj":"Tax:2697049"},{"id":"A488","pred":"tao:has_database_id","subj":"488","obj":"Tax:2697049"},{"id":"A489","pred":"tao:has_database_id","subj":"489","obj":"Tax:2697049"},{"id":"A490","pred":"tao:has_database_id","subj":"490","obj":"Tax:2697049"},{"id":"A491","pred":"tao:has_database_id","subj":"491","obj":"Tax:2697049"},{"id":"A492","pred":"tao:has_database_id","subj":"492","obj":"CVCL:6911"},{"id":"A493","pred":"tao:has_database_id","subj":"493","obj":"CVCL:6911"},{"id":"A494","pred":"tao:has_database_id","subj":"494","obj":"CVCL:0224"},{"id":"A495","pred":"tao:has_database_id","subj":"495","obj":"CVCL:0224"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Four mAbs with distinct binding profiles to SARS-CoV-1, as previously mapped by internal deletion mutagenesis study, were selected for testing to determine if they cross-react with SARS-CoV-2. A fragment containing residues 1048 to 1206 of SARS-CoV-2 S protein was expressed in 293FT cells via transient transfection and WB analysis was performed using the four mAbs, namely 2B2, 1A9, 4B12 and 1G10. As shown in Figure 2A, all four mAbs detected this fragment of SARS-CoV-2, which is consistent with the sequence alignment shown in Figure 1. Due to the easy detachment of 293FT cells, COS-7 cells were used for IF assay instead. IF analysis performed on transiently transfected COS-7 cells showed binding of the four mAbs to this S protein fragment of SARS-CoV-2 (Figure 2B). These interactions are also specific for the SARS-COV-2 S protein (1048–1206) fragment as all four mAbs did not show binding to the negative control HBcAg."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T169","span":{"begin":44,"end":52},"obj":"Disease"},{"id":"T170","span":{"begin":44,"end":48},"obj":"Disease"},{"id":"T171","span":{"begin":181,"end":189},"obj":"Disease"},{"id":"T172","span":{"begin":181,"end":185},"obj":"Disease"},{"id":"T173","span":{"begin":240,"end":248},"obj":"Disease"},{"id":"T174","span":{"begin":240,"end":244},"obj":"Disease"},{"id":"T175","span":{"begin":463,"end":471},"obj":"Disease"},{"id":"T176","span":{"begin":463,"end":467},"obj":"Disease"},{"id":"T177","span":{"begin":752,"end":760},"obj":"Disease"},{"id":"T178","span":{"begin":752,"end":756},"obj":"Disease"},{"id":"T179","span":{"begin":821,"end":825},"obj":"Disease"}],"attributes":[{"id":"A169","pred":"mondo_id","subj":"T169","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A170","pred":"mondo_id","subj":"T170","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A171","pred":"mondo_id","subj":"T171","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A172","pred":"mondo_id","subj":"T172","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A173","pred":"mondo_id","subj":"T173","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A174","pred":"mondo_id","subj":"T174","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A175","pred":"mondo_id","subj":"T175","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A176","pred":"mondo_id","subj":"T176","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A177","pred":"mondo_id","subj":"T177","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A178","pred":"mondo_id","subj":"T178","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A179","pred":"mondo_id","subj":"T179","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Four mAbs with distinct binding profiles to SARS-CoV-1, as previously mapped by internal deletion mutagenesis study, were selected for testing to determine if they cross-react with SARS-CoV-2. A fragment containing residues 1048 to 1206 of SARS-CoV-2 S protein was expressed in 293FT cells via transient transfection and WB analysis was performed using the four mAbs, namely 2B2, 1A9, 4B12 and 1G10. As shown in Figure 2A, all four mAbs detected this fragment of SARS-CoV-2, which is consistent with the sequence alignment shown in Figure 1. Due to the easy detachment of 293FT cells, COS-7 cells were used for IF assay instead. IF analysis performed on transiently transfected COS-7 cells showed binding of the four mAbs to this S protein fragment of SARS-CoV-2 (Figure 2B). These interactions are also specific for the SARS-COV-2 S protein (1048–1206) fragment as all four mAbs did not show binding to the negative control HBcAg."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T189","span":{"begin":135,"end":142},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T190","span":{"begin":193,"end":194},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T191","span":{"begin":284,"end":289},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T192","span":{"begin":394,"end":398},"obj":"http://purl.obolibrary.org/obo/CLO_0001165"},{"id":"T193","span":{"begin":419,"end":421},"obj":"http://purl.obolibrary.org/obo/CLO_0001236"},{"id":"T194","span":{"begin":578,"end":583},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T195","span":{"begin":585,"end":596},"obj":"http://purl.obolibrary.org/obo/CLO_0051656"},{"id":"T196","span":{"begin":585,"end":596},"obj":"http://purl.obolibrary.org/obo/CLO_0051657"},{"id":"T197","span":{"begin":585,"end":596},"obj":"http://purl.obolibrary.org/obo/CLO_0051658"},{"id":"T198","span":{"begin":585,"end":590},"obj":"http://purl.obolibrary.org/obo/CLO_0002597"},{"id":"T199","span":{"begin":585,"end":590},"obj":"http://purl.obolibrary.org/obo/CLO_0050508"},{"id":"T200","span":{"begin":678,"end":689},"obj":"http://purl.obolibrary.org/obo/CLO_0051656"},{"id":"T201","span":{"begin":678,"end":689},"obj":"http://purl.obolibrary.org/obo/CLO_0051657"},{"id":"T202","span":{"begin":678,"end":689},"obj":"http://purl.obolibrary.org/obo/CLO_0051658"},{"id":"T203","span":{"begin":678,"end":683},"obj":"http://purl.obolibrary.org/obo/CLO_0002597"},{"id":"T204","span":{"begin":678,"end":683},"obj":"http://purl.obolibrary.org/obo/CLO_0050508"}],"text":"Four mAbs with distinct binding profiles to SARS-CoV-1, as previously mapped by internal deletion mutagenesis study, were selected for testing to determine if they cross-react with SARS-CoV-2. A fragment containing residues 1048 to 1206 of SARS-CoV-2 S protein was expressed in 293FT cells via transient transfection and WB analysis was performed using the four mAbs, namely 2B2, 1A9, 4B12 and 1G10. As shown in Figure 2A, all four mAbs detected this fragment of SARS-CoV-2, which is consistent with the sequence alignment shown in Figure 1. Due to the easy detachment of 293FT cells, COS-7 cells were used for IF assay instead. IF analysis performed on transiently transfected COS-7 cells showed binding of the four mAbs to this S protein fragment of SARS-CoV-2 (Figure 2B). These interactions are also specific for the SARS-COV-2 S protein (1048–1206) fragment as all four mAbs did not show binding to the negative control HBcAg."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T172","span":{"begin":253,"end":260},"obj":"Chemical"},{"id":"T173","span":{"begin":732,"end":739},"obj":"Chemical"},{"id":"T174","span":{"begin":834,"end":841},"obj":"Chemical"}],"attributes":[{"id":"A172","pred":"chebi_id","subj":"T172","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A173","pred":"chebi_id","subj":"T173","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A174","pred":"chebi_id","subj":"T174","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"Four mAbs with distinct binding profiles to SARS-CoV-1, as previously mapped by internal deletion mutagenesis study, were selected for testing to determine if they cross-react with SARS-CoV-2. A fragment containing residues 1048 to 1206 of SARS-CoV-2 S protein was expressed in 293FT cells via transient transfection and WB analysis was performed using the four mAbs, namely 2B2, 1A9, 4B12 and 1G10. As shown in Figure 2A, all four mAbs detected this fragment of SARS-CoV-2, which is consistent with the sequence alignment shown in Figure 1. Due to the easy detachment of 293FT cells, COS-7 cells were used for IF assay instead. IF analysis performed on transiently transfected COS-7 cells showed binding of the four mAbs to this S protein fragment of SARS-CoV-2 (Figure 2B). These interactions are also specific for the SARS-COV-2 S protein (1048–1206) fragment as all four mAbs did not show binding to the negative control HBcAg."}

{"project":"LitCovid-PD-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":394,"end":398},"obj":"GlycoEpitope"}],"attributes":[{"id":"A1","pred":"glyco_epitope_db_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/AN0438"}],"text":"Four mAbs with distinct binding profiles to SARS-CoV-1, as previously mapped by internal deletion mutagenesis study, were selected for testing to determine if they cross-react with SARS-CoV-2. A fragment containing residues 1048 to 1206 of SARS-CoV-2 S protein was expressed in 293FT cells via transient transfection and WB analysis was performed using the four mAbs, namely 2B2, 1A9, 4B12 and 1G10. As shown in Figure 2A, all four mAbs detected this fragment of SARS-CoV-2, which is consistent with the sequence alignment shown in Figure 1. Due to the easy detachment of 293FT cells, COS-7 cells were used for IF assay instead. IF analysis performed on transiently transfected COS-7 cells showed binding of the four mAbs to this S protein fragment of SARS-CoV-2 (Figure 2B). These interactions are also specific for the SARS-COV-2 S protein (1048–1206) fragment as all four mAbs did not show binding to the negative control HBcAg."}

{"project":"LitCovid-sentences","denotations":[{"id":"T148","span":{"begin":0,"end":192},"obj":"Sentence"},{"id":"T149","span":{"begin":193,"end":399},"obj":"Sentence"},{"id":"T150","span":{"begin":400,"end":541},"obj":"Sentence"},{"id":"T151","span":{"begin":542,"end":628},"obj":"Sentence"},{"id":"T152","span":{"begin":629,"end":775},"obj":"Sentence"},{"id":"T153","span":{"begin":776,"end":931},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Four mAbs with distinct binding profiles to SARS-CoV-1, as previously mapped by internal deletion mutagenesis study, were selected for testing to determine if they cross-react with SARS-CoV-2. A fragment containing residues 1048 to 1206 of SARS-CoV-2 S protein was expressed in 293FT cells via transient transfection and WB analysis was performed using the four mAbs, namely 2B2, 1A9, 4B12 and 1G10. As shown in Figure 2A, all four mAbs detected this fragment of SARS-CoV-2, which is consistent with the sequence alignment shown in Figure 1. Due to the easy detachment of 293FT cells, COS-7 cells were used for IF assay instead. IF analysis performed on transiently transfected COS-7 cells showed binding of the four mAbs to this S protein fragment of SARS-CoV-2 (Figure 2B). These interactions are also specific for the SARS-COV-2 S protein (1048–1206) fragment as all four mAbs did not show binding to the negative control HBcAg."}