PMC:7376845 / 11826-13021 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T75","span":{"begin":98,"end":106},"obj":"Body_part"},{"id":"T76","span":{"begin":354,"end":361},"obj":"Body_part"},{"id":"T77","span":{"begin":607,"end":615},"obj":"Body_part"},{"id":"T78","span":{"begin":758,"end":761},"obj":"Body_part"}],"attributes":[{"id":"A75","pred":"fma_id","subj":"T75","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A76","pred":"fma_id","subj":"T76","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A77","pred":"fma_id","subj":"T77","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A78","pred":"fma_id","subj":"T78","obj":"http://purl.org/sig/ont/fma/fma62872"}],"text":"Sandwich ELISA\nMab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C. The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min. Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number: 40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number: 11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST. 100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C. Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid. The OD450nm was determined by a Tecan Infinite M1000 reader. Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values \u003c 0.05 were considered statistically significant."}

{"project":"LitCovid-PubTator","denotations":[{"id":"289","span":{"begin":341,"end":351},"obj":"Species"},{"id":"290","span":{"begin":431,"end":435},"obj":"Species"},{"id":"291","span":{"begin":752,"end":757},"obj":"Species"},{"id":"292","span":{"begin":62,"end":68},"obj":"Chemical"},{"id":"293","span":{"begin":76,"end":82},"obj":"Chemical"},{"id":"294","span":{"begin":854,"end":857},"obj":"Chemical"},{"id":"295","span":{"begin":930,"end":944},"obj":"Chemical"}],"attributes":[{"id":"A289","pred":"tao:has_database_id","subj":"289","obj":"Tax:2697049"},{"id":"A290","pred":"tao:has_database_id","subj":"290","obj":"Tax:119218"},{"id":"A291","pred":"tao:has_database_id","subj":"291","obj":"Tax:9606"},{"id":"A292","pred":"tao:has_database_id","subj":"292","obj":"MESH:D017693"},{"id":"A294","pred":"tao:has_database_id","subj":"294","obj":"MESH:C021758"},{"id":"A295","pred":"tao:has_database_id","subj":"295","obj":"MESH:C033158"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Sandwich ELISA\nMab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C. The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min. Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number: 40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number: 11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST. 100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C. Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid. The OD450nm was determined by a Tecan Infinite M1000 reader. Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values \u003c 0.05 were considered statistically significant."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T113","span":{"begin":341,"end":349},"obj":"Disease"},{"id":"T114","span":{"begin":341,"end":345},"obj":"Disease"}],"attributes":[{"id":"A113","pred":"mondo_id","subj":"T113","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A114","pred":"mondo_id","subj":"T114","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Sandwich ELISA\nMab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C. The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min. Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number: 40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number: 11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST. 100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C. Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid. The OD450nm was determined by a Tecan Infinite M1000 reader. Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values \u003c 0.05 were considered statistically significant."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T144","span":{"begin":70,"end":72},"obj":"http://purl.obolibrary.org/obo/CLO_0001302"},{"id":"T145","span":{"begin":143,"end":144},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T146","span":{"begin":192,"end":196},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T147","span":{"begin":629,"end":630},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T148","span":{"begin":742,"end":746},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T149","span":{"begin":752,"end":761},"obj":"http://purl.obolibrary.org/obo/CLO_0053695"},{"id":"T150","span":{"begin":976,"end":977},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T151","span":{"begin":1066,"end":1072},"obj":"http://purl.obolibrary.org/obo/UBERON_0002415"},{"id":"T152","span":{"begin":1085,"end":1089},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"}],"text":"Sandwich ELISA\nMab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C. The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min. Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number: 40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number: 11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST. 100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C. Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid. The OD450nm was determined by a Tecan Infinite M1000 reader. Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values \u003c 0.05 were considered statistically significant."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T111","span":{"begin":48,"end":54},"obj":"Chemical"},{"id":"T112","span":{"begin":62,"end":68},"obj":"Chemical"},{"id":"T113","span":{"begin":76,"end":82},"obj":"Chemical"},{"id":"T114","span":{"begin":354,"end":361},"obj":"Chemical"},{"id":"T115","span":{"begin":436,"end":438},"obj":"Chemical"},{"id":"T116","span":{"begin":930,"end":944},"obj":"Chemical"},{"id":"T117","span":{"begin":940,"end":944},"obj":"Chemical"}],"attributes":[{"id":"A111","pred":"chebi_id","subj":"T111","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A112","pred":"chebi_id","subj":"T112","obj":"http://purl.obolibrary.org/obo/CHEBI_32139"},{"id":"A113","pred":"chebi_id","subj":"T113","obj":"http://purl.obolibrary.org/obo/CHEBI_29377"},{"id":"A114","pred":"chebi_id","subj":"T114","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A115","pred":"chebi_id","subj":"T115","obj":"http://purl.obolibrary.org/obo/CHEBI_73924"},{"id":"A116","pred":"chebi_id","subj":"T116","obj":"http://purl.obolibrary.org/obo/CHEBI_26836"},{"id":"A117","pred":"chebi_id","subj":"T117","obj":"http://purl.obolibrary.org/obo/CHEBI_37527"}],"text":"Sandwich ELISA\nMab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C. The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min. Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number: 40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number: 11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST. 100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C. Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid. The OD450nm was determined by a Tecan Infinite M1000 reader. Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values \u003c 0.05 were considered statistically significant."}

{"project":"LitCovid-sentences","denotations":[{"id":"T82","span":{"begin":0,"end":14},"obj":"Sentence"},{"id":"T83","span":{"begin":15,"end":197},"obj":"Sentence"},{"id":"T84","span":{"begin":198,"end":304},"obj":"Sentence"},{"id":"T85","span":{"begin":305,"end":402},"obj":"Sentence"},{"id":"T86","span":{"begin":403,"end":479},"obj":"Sentence"},{"id":"T87","span":{"begin":480,"end":589},"obj":"Sentence"},{"id":"T88","span":{"begin":590,"end":819},"obj":"Sentence"},{"id":"T89","span":{"begin":820,"end":945},"obj":"Sentence"},{"id":"T90","span":{"begin":946,"end":1006},"obj":"Sentence"},{"id":"T91","span":{"begin":1007,"end":1195},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Sandwich ELISA\nMab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C. The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min. Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number: 40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number: 11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST. 100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C. Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid. The OD450nm was determined by a Tecan Infinite M1000 reader. Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values \u003c 0.05 were considered statistically significant."}