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PMC:7376845 / 11826-13021 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T75 98-106 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T76 354-361 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T77 607-615 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T78 758-761 Body_part denotes IgG http://purl.org/sig/ont/fma/fma62872

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
289 341-351 Species denotes SARS-CoV-2 Tax:2697049
290 431-435 Species denotes H7N7 Tax:119218
291 752-757 Species denotes human Tax:9606
292 62-68 Chemical denotes NaHCO3 MESH:D017693
293 76-82 Chemical denotes Na2CO3
294 854-857 Chemical denotes TMB MESH:C021758
295 930-944 Chemical denotes sulphuric acid MESH:C033158

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T113 341-349 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T114 341-345 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T144 70-72 http://purl.obolibrary.org/obo/CLO_0001302 denotes 34
T145 143-144 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T146 192-196 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T147 629-630 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T148 742-746 http://purl.obolibrary.org/obo/NCBITaxon_9925 denotes goat
T149 752-761 http://purl.obolibrary.org/obo/CLO_0053695 denotes human IgG
T150 976-977 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T151 1066-1072 http://purl.obolibrary.org/obo/UBERON_0002415 denotes tailed
T152 1085-1089 http://purl.obolibrary.org/obo/UBERON_0000473 denotes test

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T111 48-54 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T112 62-68 Chemical denotes NaHCO3 http://purl.obolibrary.org/obo/CHEBI_32139
T113 76-82 Chemical denotes Na2CO3 http://purl.obolibrary.org/obo/CHEBI_29377
T114 354-361 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T115 436-438 Chemical denotes HA http://purl.obolibrary.org/obo/CHEBI_73924
T116 930-944 Chemical denotes sulphuric acid http://purl.obolibrary.org/obo/CHEBI_26836
T117 940-944 Chemical denotes acid http://purl.obolibrary.org/obo/CHEBI_37527

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T82 0-14 Sentence denotes Sandwich ELISA
T83 15-197 Sentence denotes Mab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C.
T84 198-304 Sentence denotes The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min.
T85 305-402 Sentence denotes Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number:
T86 403-479 Sentence denotes 40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number:
T87 480-589 Sentence denotes 11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST.
T88 590-819 Sentence denotes 100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C.
T89 820-945 Sentence denotes Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid.
T90 946-1006 Sentence denotes The OD450nm was determined by a Tecan Infinite M1000 reader.
T91 1007-1195 Sentence denotes Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values < 0.05 were considered statistically significant.