PMC:7354481 / 11561-15193
Annnotations
LitCovid-PD-FMA-UBERON
Id | Subject | Object | Predicate | Lexical cue | fma_id |
---|---|---|---|---|---|
T70 | 30-36 | Body_part | denotes | Genome | http://purl.org/sig/ont/fma/fma84116 |
T71 | 62-68 | Body_part | denotes | genome | http://purl.org/sig/ont/fma/fma84116 |
T72 | 320-326 | Body_part | denotes | genome | http://purl.org/sig/ont/fma/fma84116 |
T73 | 657-662 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T74 | 909-915 | Body_part | denotes | genome | http://purl.org/sig/ont/fma/fma84116 |
T75 | 1009-1011 | Body_part | denotes | V3 | http://purl.org/sig/ont/fma/fma13442 |
T76 | 1312-1318 | Body_part | denotes | genome | http://purl.org/sig/ont/fma/fma84116 |
T77 | 1626-1630 | Body_part | denotes | lung | http://purl.org/sig/ont/fma/fma7195 |
T78 | 1754-1758 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
T79 | 2097-2101 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
T80 | 2144-2148 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
T81 | 2470-2476 | Body_part | denotes | genome | http://purl.org/sig/ont/fma/fma84116 |
T82 | 3183-3187 | Body_part | denotes | gene | http://purl.org/sig/ont/fma/fma74402 |
LitCovid-PD-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T12 | 1626-1630 | Body_part | denotes | lung | http://purl.obolibrary.org/obo/UBERON_0002048 |
LitCovid-PD-MONDO
Id | Subject | Object | Predicate | Lexical cue | mondo_id |
---|---|---|---|---|---|
T47 | 51-59 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T48 | 136-140 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T49 | 309-317 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T50 | 582-590 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T51 | 898-906 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T52 | 1645-1653 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T53 | 1717-1725 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T54 | 2499-2503 | Disease | denotes | STAR | http://purl.obolibrary.org/obo/MONDO_0010408 |
T55 | 2720-2723 | Disease | denotes | MDS | http://purl.obolibrary.org/obo/MONDO_0009532|http://purl.obolibrary.org/obo/MONDO_0018881 |
T57 | 2984-2987 | Disease | denotes | PAM | http://purl.obolibrary.org/obo/MONDO_0018959 |
LitCovid-PD-CLO
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T108 | 418-424 | http://purl.obolibrary.org/obo/NCBITaxon_9005 | denotes | Turkey |
T109 | 434-440 | http://purl.obolibrary.org/obo/NCBITaxon_9005 | denotes | Turkey |
T110 | 580-581 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T111 | 614-615 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T112 | 649-662 | http://purl.obolibrary.org/obo/CLO_0051719 | denotes | Vero E6 cells |
T113 | 691-697 | http://purl.obolibrary.org/obo/NCBITaxon_9005 | denotes | Turkey |
T114 | 920-925 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | human |
T115 | 1009-1011 | http://purl.obolibrary.org/obo/CLO_0050428 | denotes | V3 |
T116 | 1087-1095 | http://purl.obolibrary.org/obo/CLO_0009985 | denotes | focusing |
T117 | 1626-1630 | http://purl.obolibrary.org/obo/UBERON_0002048 | denotes | lung |
T118 | 1626-1630 | http://www.ebi.ac.uk/efo/EFO_0000934 | denotes | lung |
T119 | 1749-1753 | http://purl.obolibrary.org/obo/CLO_0001601 | denotes | A549 |
T120 | 1749-1753 | http://purl.obolibrary.org/obo/CLO_0050025 | denotes | A549 |
T121 | 1749-1753 | http://purl.obolibrary.org/obo/CLO_0054264 | denotes | A549 |
T122 | 1749-1753 | http://purl.obolibrary.org/obo/CLO_0054265 | denotes | A549 |
T123 | 1749-1753 | http://purl.obolibrary.org/obo/CLO_0054266 | denotes | A549 |
T124 | 1749-1753 | http://purl.obolibrary.org/obo/CLO_0054267 | denotes | A549 |
T125 | 1749-1753 | http://purl.obolibrary.org/obo/CLO_0054268 | denotes | A549 |
T126 | 1749-1753 | http://purl.obolibrary.org/obo/CLO_0054269 | denotes | A549 |
T127 | 1754-1764 | http://purl.obolibrary.org/obo/CLO_0000031 | denotes | cell lines |
T128 | 2097-2107 | http://purl.obolibrary.org/obo/CLO_0000031 | denotes | cell lines |
T129 | 2111-2112 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T130 | 2144-2154 | http://purl.obolibrary.org/obo/CLO_0000031 | denotes | cell lines |
T131 | 2254-2255 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T132 | 2457-2469 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | Homo sapiens |
T133 | 2505-2507 | http://purl.obolibrary.org/obo/CLO_0050509 | denotes | 27 |
T134 | 2903-2908 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | genes |
T135 | 2959-2964 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | genes |
T136 | 3183-3187 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | gene |
T137 | 3512-3514 | http://purl.obolibrary.org/obo/CLO_0001313 | denotes | 36 |
T138 | 3578-3579 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T139 | 3598-3603 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | genes |
LitCovid-PD-CHEBI
Id | Subject | Object | Predicate | Lexical cue | chebi_id |
---|---|---|---|---|---|
T20 | 1518-1525 | Chemical | denotes | Pathway | http://purl.obolibrary.org/obo/CHEBI_34922 |
T21 | 2984-2987 | Chemical | denotes | PAM | http://purl.obolibrary.org/obo/CHEBI_51135|http://purl.obolibrary.org/obo/CHEBI_60882 |
LitCovid-sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T72 | 0-2 | Sentence | denotes | 2. |
T73 | 3-23 | Sentence | denotes | Material and Methods |
T74 | 25-29 | Sentence | denotes | 2.1. |
T75 | 30-46 | Sentence | denotes | Genome Sequences |
T76 | 47-304 | Sentence | denotes | The SARS-CoV-2 genome sequences from China, Italy, Spain (Valencia), and those for MERS, SARS, OC43 and 229E were obtained from NCBI (GenBank: NC_045512.2, LC528232.1, MT066156.1, MT198652.2, KT225476.2, NC_004718.3, NC_006213.1, NC_002645.1, respectively). |
T77 | 305-566 | Sentence | denotes | The SARS-CoV-2 genome sequences from England (hCoV-19/England/20136087804/2020|EPI_ISL_420910, no treatment) and Turkey (hCoV-19/Turkey/GLAB-CoV008/2020) were obtained from the China National Bioinformatics Center, GISAID database [23] (https://www.gisaid.org). |
T78 | 567-789 | Sentence | denotes | In addition, a SARS-CoV-2 strain isolated from a Turkish patient, and infected to Vero E6 cells passage 4 sequence (hCoV-19/Turkey/ERAGEM-001/2020;) was used for alignment studies with the miRBase mature miRNA search tool. |
T79 | 791-810 | Sentence | denotes | 2.2. miR Prediction |
T80 | 811-989 | Sentence | denotes | The miRTarget and miRBase programmes were used to predict the similarities between the SARS-CoV-2 genome and human miRs; e-value <10 and score >70 were considered as significant. |
T81 | 990-1135 | Sentence | denotes | DianaTools miRPath V3 were then used to create heat maps for pathways affected by selected miRs, focusing on the microT-CDS version 5.0 database. |
T82 | 1136-1196 | Sentence | denotes | The p value threshold was 0.05 and microT threshold was 0.8. |
T83 | 1197-1254 | Sentence | denotes | Heatmap analysis was done with pathway intersection [24]. |
T84 | 1256-1260 | Sentence | denotes | 2.3. |
T85 | 1261-1305 | Sentence | denotes | Mutational Analysis of Potential miRNA Sites |
T86 | 1306-1511 | Sentence | denotes | Viral genome sequencing data was obtained from the GISAID database (https://www.gisaid.org), and analysed as multiple sequence alignments using the Clustal Omega at EBI (www.ebi.ac.uk/Tools/msa/clustalo/). |
T87 | 1513-1517 | Sentence | denotes | 2.4. |
T88 | 1518-1534 | Sentence | denotes | Pathway Analysis |
T89 | 1535-1765 | Sentence | denotes | Bioproject data was obtained from PRJNA615032 bioproject trancriptome data, which includes lung biopsies from SARS-CoV-2-infected patients and healthy volunteers as well as mock and SARS-CoV-2-transfected NHEB and A549 cell lines. |
T90 | 1766-1924 | Sentence | denotes | The data have been deposited with links to BioProject accession number PRJNA615032 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/). |
T91 | 1925-2001 | Sentence | denotes | All the selected data were reanalysed at the Rosalind bioinformatics server. |
T92 | 2002-2186 | Sentence | denotes | Data analysis was performed according to 1.5 fold change between untransfected and transfected cell lines in a data pool calculation for both cell lines at p < 0.05 significance level. |
T93 | 2187-2342 | Sentence | denotes | Data was analyzed by Rosalind (https://rosalind.onramp.bio/), with a HyperScale architecture developed by OnRamp BioInformatics, Inc. (San Diego, CA, USA). |
T94 | 2343-2382 | Sentence | denotes | Reads were trimmed using cutadapt [25]. |
T95 | 2383-2430 | Sentence | denotes | Quality scores were assessed using FastQC [26]. |
T96 | 2431-2509 | Sentence | denotes | Reads were aligned to the Homo sapiens genome built by GRCh38 using STAR [27]. |
T97 | 2510-2644 | Sentence | denotes | Individual sample reads were quantified using HTseq [28] and normalized via relative log expression (RLE) using DESeq2 R library [29]. |
T98 | 2645-2785 | Sentence | denotes | Read distribution percentages, violin plots, identity heatmaps, and sample MDS plots were generated as part of the QC step using RSeQC [30]. |
T99 | 2786-2888 | Sentence | denotes | DEseq2 was also used to calculate fold changes and p-values and perform optional covariate correction. |
T100 | 2889-3106 | Sentence | denotes | Clustering of genes for the final heatmap of differentially expressed genes was done using the PAM (partitioning around medoids) method using the fpc R library (https://cran.r-project.org/web/packages/fpc/index.html). |
T101 | 3107-3237 | Sentence | denotes | Hypergeometric distribution was used to analyze the enrichment of pathways, gene ontology, domain structure, and other ontologies. |
T102 | 3238-3383 | Sentence | denotes | The topGO R library [31], was used to determine local similarities and dependencies between GO terms in order to perform Elim pruning correction. |
T103 | 3384-3539 | Sentence | denotes | Several database sources were referenced for enrichment analysis, including Interpro [32], NCBI [33], MSigDB [34,35], REACTOME [36], and WikiPathways [37]. |
T104 | 3540-3632 | Sentence | denotes | Enrichment was calculated relative to a set of background genes relevant for the experiment. |
2_test
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
32512929-24935956-144200712 | 537-539 | 24935956 | denotes | 23 |
32512929-28382917-144200713 | 1250-1252 | 28382917 | denotes | 24 |
32512929-25977294-144200714 | 2378-2380 | 25977294 | denotes | 25 |
32512929-23104886-144200715 | 2563-2565 | 23104886 | denotes | 28 |
32512929-25260700-144200716 | 2640-2642 | 25260700 | denotes | 29 |
32512929-25516281-144200717 | 2781-2783 | 25516281 | denotes | 30 |
32512929-22743226-144200718 | 3259-3261 | 22743226 | denotes | 31 |
32512929-30398656-144200719 | 3481-3483 | 30398656 | denotes | 33 |
32512929-19854944-144200720 | 3494-3496 | 19854944 | denotes | 34 |
32512929-29145629-144200721 | 3535-3537 | 29145629 | denotes | 37 |
LitCovid-PubTator
Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
---|---|---|---|---|---|
199 | 51-61 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
200 | 309-319 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
201 | 351-358 | Species | denotes | hCoV-19 | Tax:2697049 |
202 | 418-424 | Species | denotes | Turkey | Tax:9103 |
203 | 426-433 | Species | denotes | hCoV-19 | Tax:2697049 |
204 | 434-440 | Species | denotes | Turkey | Tax:9103 |
205 | 582-592 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
206 | 624-631 | Species | denotes | patient | Tax:9606 |
207 | 683-690 | Species | denotes | hCoV-19 | Tax:2697049 |
208 | 691-697 | Species | denotes | Turkey | Tax:9103 |
209 | 142-146 | Species | denotes | OC43 | Tax:31631 |
210 | 151-155 | Species | denotes | 229E | Tax:11137 |
211 | 446-449 | Species | denotes | CoV | Tax:11118 |
212 | 130-134 | Disease | denotes | MERS | MESH:D018352 |
213 | 637-645 | Disease | denotes | infected | MESH:D007239 |
216 | 898-908 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
217 | 920-925 | Species | denotes | human | Tax:9606 |
219 | 1471-1474 | Gene | denotes | EBI | Gene:6907 |
226 | 2499-2503 | Gene | denotes | STAR | Gene:6770 |
227 | 1665-1673 | Species | denotes | patients | Tax:9606 |
228 | 1717-1727 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
229 | 2457-2469 | Species | denotes | Homo sapiens | Tax:9606 |
230 | 1645-1664 | Disease | denotes | SARS-CoV-2-infected | MESH:C000657245 |
231 | 1749-1753 | CellLine | denotes | A549 | CVCL:0023 |