PMC:7321036 / 99549-100349 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T1","span":{"begin":116,"end":123},"obj":"Body_part"},{"id":"T2","span":{"begin":258,"end":262},"obj":"Body_part"},{"id":"T3","span":{"begin":645,"end":652},"obj":"Body_part"},{"id":"T4","span":{"begin":744,"end":747},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"fma_id","subj":"T1","obj":"http://purl.org/sig/ont/fma/fma84116"},{"id":"A2","pred":"fma_id","subj":"T2","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A3","pred":"fma_id","subj":"T3","obj":"http://purl.org/sig/ont/fma/fma84116"},{"id":"A4","pred":"fma_id","subj":"T4","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"SARS-CoV-2 viral quantification via RT-qPCR in presence of inhibitors\n(Institut Pasteur, Paris). Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). SARS-CoV-2 specific primers targeting the N gene region: 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T384","span":{"begin":0,"end":8},"obj":"Disease"},{"id":"T385","span":{"begin":214,"end":222},"obj":"Disease"}],"attributes":[{"id":"A384","pred":"mondo_id","subj":"T384","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A385","pred":"mondo_id","subj":"T385","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"SARS-CoV-2 viral quantification via RT-qPCR in presence of inhibitors\n(Institut Pasteur, Paris). Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). SARS-CoV-2 specific primers targeting the N gene region: 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1","span":{"begin":258,"end":262},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T2","span":{"begin":722,"end":723},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T3","span":{"begin":761,"end":762},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T4","span":{"begin":780,"end":781},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"SARS-CoV-2 viral quantification via RT-qPCR in presence of inhibitors\n(Institut Pasteur, Paris). Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). SARS-CoV-2 specific primers targeting the N gene region: 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T64554","span":{"begin":59,"end":69},"obj":"Chemical"}],"attributes":[{"id":"A22588","pred":"chebi_id","subj":"T64554","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"}],"text":"SARS-CoV-2 viral quantification via RT-qPCR in presence of inhibitors\n(Institut Pasteur, Paris). Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). SARS-CoV-2 specific primers targeting the N gene region: 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer."}

{"project":"LitCovid-PubTator","denotations":[{"id":"2283","span":{"begin":0,"end":10},"obj":"Species"},{"id":"2286","span":{"begin":256,"end":257},"obj":"Gene"},{"id":"2287","span":{"begin":214,"end":224},"obj":"Species"}],"attributes":[{"id":"A2283","pred":"tao:has_database_id","subj":"2283","obj":"Tax:2697049"},{"id":"A2286","pred":"tao:has_database_id","subj":"2286","obj":"Gene:43740575"},{"id":"A2287","pred":"tao:has_database_id","subj":"2287","obj":"Tax:2697049"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"SARS-CoV-2 viral quantification via RT-qPCR in presence of inhibitors\n(Institut Pasteur, Paris). Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). SARS-CoV-2 specific primers targeting the N gene region: 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer."}

{"project":"LitCovid-sentences","denotations":[{"id":"T869","span":{"begin":0,"end":69},"obj":"Sentence"},{"id":"T870","span":{"begin":70,"end":96},"obj":"Sentence"},{"id":"T871","span":{"begin":97,"end":213},"obj":"Sentence"},{"id":"T872","span":{"begin":214,"end":270},"obj":"Sentence"},{"id":"T873","span":{"begin":271,"end":521},"obj":"Sentence"},{"id":"T874","span":{"begin":522,"end":624},"obj":"Sentence"},{"id":"T875","span":{"begin":625,"end":800},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"SARS-CoV-2 viral quantification via RT-qPCR in presence of inhibitors\n(Institut Pasteur, Paris). Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). SARS-CoV-2 specific primers targeting the N gene region: 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer."}

{"project":"2_test","denotations":[{"id":"32645325-32225176-20773112","span":{"begin":207,"end":211},"obj":"32225176"},{"id":"32645325-32031583-20773113","span":{"begin":363,"end":367},"obj":"32031583"}],"text":"SARS-CoV-2 viral quantification via RT-qPCR in presence of inhibitors\n(Institut Pasteur, Paris). Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). SARS-CoV-2 specific primers targeting the N gene region: 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer."}