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PMC:7321036 / 99549-100349 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T1 116-123 Body_part denotes genomes http://purl.org/sig/ont/fma/fma84116
T2 258-262 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T3 645-652 Body_part denotes genomes http://purl.org/sig/ont/fma/fma84116
T4 744-747 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T384 0-8 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T385 214-222 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T1 258-262 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T2 722-723 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T3 761-762 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T4 780-781 http://purl.obolibrary.org/obo/CLO_0001020 denotes a

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T64554 59-69 Chemical denotes inhibitors http://purl.obolibrary.org/obo/CHEBI_35222

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
2283 0-10 Species denotes SARS-CoV-2 Tax:2697049
2286 256-257 Gene denotes N Gene:43740575
2287 214-224 Species denotes SARS-CoV-2 Tax:2697049

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T869 0-69 Sentence denotes SARS-CoV-2 viral quantification via RT-qPCR in presence of inhibitors
T870 70-96 Sentence denotes (Institut Pasteur, Paris).
T871 97-213 Sentence denotes Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020).
T872 214-270 Sentence denotes SARS-CoV-2 specific primers targeting the N gene region:
T873 271-521 Sentence denotes 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions:
T874 522-624 Sentence denotes 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute.
T875 625-800 Sentence denotes The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer.

2_test

Id Subject Object Predicate Lexical cue
32645325-32225176-20773112 207-211 32225176 denotes 2020
32645325-32031583-20773113 363-367 32031583 denotes 2020