PMC:7321036 / 90514-90772 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T191","span":{"begin":71,"end":83},"obj":"SO:0000704"}],"text":"To produce lentiviruses, HEK293T cells (50% confluent in a T175 flask) were transfected with 5 μg of each of the pLVX-TetOne-Puro lentiviral plasmids, 3.33 μg Gag-Pol packaging construct, and 1.66 μg VSV-G envelope (pMD2.G, Addgene) using PolyJet (SignaGen)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T643","span":{"begin":33,"end":38},"obj":"Body_part"},{"id":"T644","span":{"begin":159,"end":162},"obj":"Body_part"}],"attributes":[{"id":"A643","pred":"fma_id","subj":"T643","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A644","pred":"fma_id","subj":"T644","obj":"http://purl.org/sig/ont/fma/fma63011"}],"text":"To produce lentiviruses, HEK293T cells (50% confluent in a T175 flask) were transfected with 5 μg of each of the pLVX-TetOne-Puro lentiviral plasmids, 3.33 μg Gag-Pol packaging construct, and 1.66 μg VSV-G envelope (pMD2.G, Addgene) using PolyJet (SignaGen)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1239","span":{"begin":33,"end":38},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1240","span":{"begin":57,"end":58},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"To produce lentiviruses, HEK293T cells (50% confluent in a T175 flask) were transfected with 5 μg of each of the pLVX-TetOne-Puro lentiviral plasmids, 3.33 μg Gag-Pol packaging construct, and 1.66 μg VSV-G envelope (pMD2.G, Addgene) using PolyJet (SignaGen)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"2039","span":{"begin":200,"end":203},"obj":"Species"},{"id":"2054","span":{"begin":25,"end":32},"obj":"CellLine"}],"attributes":[{"id":"A2039","pred":"tao:has_database_id","subj":"2039","obj":"Tax:11276"},{"id":"A2054","pred":"tao:has_database_id","subj":"2054","obj":"CVCL:0063"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"To produce lentiviruses, HEK293T cells (50% confluent in a T175 flask) were transfected with 5 μg of each of the pLVX-TetOne-Puro lentiviral plasmids, 3.33 μg Gag-Pol packaging construct, and 1.66 μg VSV-G envelope (pMD2.G, Addgene) using PolyJet (SignaGen)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T798","span":{"begin":0,"end":258},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To produce lentiviruses, HEK293T cells (50% confluent in a T175 flask) were transfected with 5 μg of each of the pLVX-TetOne-Puro lentiviral plasmids, 3.33 μg Gag-Pol packaging construct, and 1.66 μg VSV-G envelope (pMD2.G, Addgene) using PolyJet (SignaGen)."}