PMC:7321036 / 100351-100975 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7321036","sourcedb":"PMC","sourceid":"7321036","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7321036","text":"SARS-CoV-2 titration by plaque assay\n(Institut Pasteur, Paris). Vero E6 cells were seeded in 24-well plates at a concentration of 7.5x104 cells/well. The following day, serial dilutions were performed in serum-free MEM media. After 1 hour absorption at 37°C, 2x overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS / MEM media and 0.05% (w/v) Agarose (all Thermo Fisher Scientific) to achieve a semi-solid overlay. Plaque assays were incubated at 37°C for 3 days. Samples were fixed using 4% Formalin (Sigma Aldrich) and plaques were visualized using crystal Violet solution (Sigma Aldrich).","divisions":[{"label":"title","span":{"begin":0,"end":36}}],"tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T5","span":{"begin":72,"end":77},"obj":"Body_part"},{"id":"T6","span":{"begin":138,"end":143},"obj":"Body_part"},{"id":"T7","span":{"begin":204,"end":209},"obj":"Body_part"}],"attributes":[{"id":"A5","pred":"fma_id","subj":"T5","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A6","pred":"fma_id","subj":"T6","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A7","pred":"fma_id","subj":"T7","obj":"http://purl.org/sig/ont/fma/fma63083"},{"subj":"T5","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T6","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T7","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T30","span":{"begin":204,"end":209},"obj":"Body_part"}],"attributes":[{"id":"A30","pred":"uberon_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"subj":"T30","pred":"source","obj":"LitCovid-PD-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T386","span":{"begin":0,"end":8},"obj":"Disease"}],"attributes":[{"id":"A386","pred":"mondo_id","subj":"T386","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"subj":"T386","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T5","span":{"begin":64,"end":77},"obj":"http://purl.obolibrary.org/obo/CLO_0051719"},{"id":"T6","span":{"begin":111,"end":112},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T7","span":{"begin":138,"end":143},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T8","span":{"begin":310,"end":311},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T9","span":{"begin":371,"end":374},"obj":"http://purl.obolibrary.org/obo/CLO_0050644"},{"id":"T10","span":{"begin":426,"end":427},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"attributes":[{"subj":"T5","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T6","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T7","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T8","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T9","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T10","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T5336","span":{"begin":376,"end":383},"obj":"Chemical"},{"id":"T98246","span":{"begin":525,"end":533},"obj":"Chemical"},{"id":"T9421","span":{"begin":599,"end":607},"obj":"Chemical"}],"attributes":[{"id":"A5204","pred":"chebi_id","subj":"T5336","obj":"http://purl.obolibrary.org/obo/CHEBI_2511"},{"id":"A24177","pred":"chebi_id","subj":"T98246","obj":"http://purl.obolibrary.org/obo/CHEBI_16842"},{"id":"A24726","pred":"chebi_id","subj":"T9421","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"subj":"T5336","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T98246","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T9421","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"2289","span":{"begin":0,"end":10},"obj":"Species"},{"id":"2295","span":{"begin":215,"end":224},"obj":"Chemical"},{"id":"2296","span":{"begin":376,"end":383},"obj":"Chemical"},{"id":"2297","span":{"begin":525,"end":533},"obj":"Chemical"},{"id":"2298","span":{"begin":584,"end":598},"obj":"Chemical"},{"id":"2299","span":{"begin":69,"end":71},"obj":"CellLine"}],"attributes":[{"id":"A2289","pred":"tao:has_database_id","subj":"2289","obj":"Tax:2697049"},{"id":"A2296","pred":"tao:has_database_id","subj":"2296","obj":"MESH:D012685"},{"id":"A2297","pred":"tao:has_database_id","subj":"2297","obj":"MESH:D005557"},{"id":"A2298","pred":"tao:has_database_id","subj":"2298","obj":"MESH:D005840"},{"id":"A2299","pred":"tao:has_database_id","subj":"2299","obj":"CVCL:4582"},{"subj":"2289","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2295","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2296","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2297","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2298","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2299","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T876","span":{"begin":0,"end":36},"obj":"Sentence"},{"id":"T877","span":{"begin":37,"end":63},"obj":"Sentence"},{"id":"T878","span":{"begin":64,"end":149},"obj":"Sentence"},{"id":"T879","span":{"begin":150,"end":225},"obj":"Sentence"},{"id":"T880","span":{"begin":226,"end":447},"obj":"Sentence"},{"id":"T881","span":{"begin":448,"end":496},"obj":"Sentence"},{"id":"T882","span":{"begin":497,"end":624},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T876","pred":"source","obj":"LitCovid-sentences"},{"subj":"T877","pred":"source","obj":"LitCovid-sentences"},{"subj":"T878","pred":"source","obj":"LitCovid-sentences"},{"subj":"T879","pred":"source","obj":"LitCovid-sentences"},{"subj":"T880","pred":"source","obj":"LitCovid-sentences"},{"subj":"T881","pred":"source","obj":"LitCovid-sentences"},{"subj":"T882","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#93eca0","default":true},{"id":"LitCovid-PD-UBERON","color":"#ec939f"},{"id":"LitCovid-PD-MONDO","color":"#93b9ec"},{"id":"LitCovid-PD-CLO","color":"#d3ec93"},{"id":"LitCovid-PD-CHEBI","color":"#eb93ec"},{"id":"LitCovid-PubTator","color":"#93ecd1"},{"id":"LitCovid-sentences","color":"#ecb793"}]}]}}