PMC:7321036 / 100351-100975 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T5 72-77 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T6 138-143 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T7 204-209 Body_part denotes serum http://purl.org/sig/ont/fma/fma63083

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T30 204-209 Body_part denotes serum http://purl.obolibrary.org/obo/UBERON_0001977

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T386 0-8 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T5 64-77 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T6 111-112 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T7 138-143 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8 310-311 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T9 371-374 http://purl.obolibrary.org/obo/CLO_0050644 denotes w/v
T10 426-427 http://purl.obolibrary.org/obo/CLO_0001020 denotes a

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T5336 376-383 Chemical denotes Agarose http://purl.obolibrary.org/obo/CHEBI_2511
T98246 525-533 Chemical denotes Formalin http://purl.obolibrary.org/obo/CHEBI_16842
T9421 599-607 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
2289 0-10 Species denotes SARS-CoV-2 Tax:2697049
2295 215-224 Chemical denotes MEM media
2296 376-383 Chemical denotes Agarose MESH:D012685
2297 525-533 Chemical denotes Formalin MESH:D005557
2298 584-598 Chemical denotes crystal Violet MESH:D005840
2299 69-71 CellLine denotes E6 CVCL:4582

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T876 0-36 Sentence denotes SARS-CoV-2 titration by plaque assay
T877 37-63 Sentence denotes (Institut Pasteur, Paris).
T878 64-149 Sentence denotes Vero E6 cells were seeded in 24-well plates at a concentration of 7.5x104 cells/well.
T879 150-225 Sentence denotes The following day, serial dilutions were performed in serum-free MEM media.
T880 226-447 Sentence denotes After 1 hour absorption at 37°C, 2x overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS / MEM media and 0.05% (w/v) Agarose (all Thermo Fisher Scientific) to achieve a semi-solid overlay.
T881 448-496 Sentence denotes Plaque assays were incubated at 37°C for 3 days.
T882 497-624 Sentence denotes Samples were fixed using 4% Formalin (Sigma Aldrich) and plaques were visualized using crystal Violet solution (Sigma Aldrich).