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PMC:7291971 / 85505-86967 JSONTXT

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LitCovid-PMC-OGER-BB

Id Subject Object Predicate Lexical cue
T1693 39-41 CHEBI:29238;CHEBI:29238 denotes 3H
T1694 202-209 CHEBI:60004;CHEBI:60004 denotes mixture
T1695 217-221 CHEBI:9754;CHEBI:9754 denotes Tris
T1696 222-225 CHEBI:17883;CHEBI:17883 denotes HCl
T1697 241-244 CHEBI:18320;CHEBI:18320 denotes DTT
T1698 251-256 CHEBI:6636;CHEBI:6636 denotes MgCl2
T1699 278-280 CHEBI:29238;CHEBI:29238 denotes 3H
T1700 352-360 SP_10 denotes SARS-CoV
T1701 361-366 PR:000000125 denotes nsp14
T1702 376-384 NCBITaxon:7742 denotes vaccinia
T1703 385-390 NCBITaxon:10239 denotes virus
T1704 426-431 SP_6;NCBITaxon:9606 denotes human
T1705 446-451 PR:O43148 denotes hRNMT
T1706 496-505 CHEBI:36357;CHEBI:36357 denotes compounds
T1707 524-528 CHEBI:51231;CHEBI:51231 denotes DMSO
T1708 539-543 CHEBI:51231;CHEBI:51231 denotes DMSO
T1709 674-678 CHEBI:51231;CHEBI:51231 denotes DMSO
T1710 690-698 CHEBI:60004;CHEBI:60004 denotes mixtures
T1711 763-768 CHEBI:15377;CHEBI:15377 denotes water
T1712 798-815 CHEBI:35702;CHEBI:35702 denotes diethylaminoethyl
T1713 950-966 CHEBI:63050;CHEBI:63050 denotes ammonium formate
T1714 986-991 CHEBI:15377;CHEBI:15377 denotes water
T1715 1006-1013 CHEBI:16236;CHEBI:16236 denotes ethanol
T1716 1076-1078 CHEBI:29238;CHEBI:29238 denotes 3H
T1717 1394-1402 CHEBI:36357;CHEBI:36357 denotes compound
T18522 39-41 CHEBI:29238;CHEBI:29238 denotes 3H
T89446 202-209 CHEBI:60004;CHEBI:60004 denotes mixture
T7568 217-221 CHEBI:9754;CHEBI:9754 denotes Tris
T90528 222-225 CHEBI:17883;CHEBI:17883 denotes HCl
T32618 241-244 CHEBI:18320;CHEBI:18320 denotes DTT
T80620 251-256 CHEBI:6636;CHEBI:6636 denotes MgCl2
T65164 278-280 CHEBI:29238;CHEBI:29238 denotes 3H
T99852 352-360 SP_10 denotes SARS-CoV
T89465 361-366 PR:000000125 denotes nsp14
T92733 376-384 NCBITaxon:7742 denotes vaccinia
T32742 385-390 NCBITaxon:10239 denotes virus
T40449 426-431 SP_6;NCBITaxon:9606 denotes human
T9798 446-451 PR:O43148 denotes hRNMT
T22704 496-505 CHEBI:36357;CHEBI:36357 denotes compounds
T3161 524-528 CHEBI:51231;CHEBI:51231 denotes DMSO
T66008 539-543 CHEBI:51231;CHEBI:51231 denotes DMSO
T46228 674-678 CHEBI:51231;CHEBI:51231 denotes DMSO
T24685 690-698 CHEBI:60004;CHEBI:60004 denotes mixtures
T53961 763-768 CHEBI:15377;CHEBI:15377 denotes water
T66324 798-815 CHEBI:35702;CHEBI:35702 denotes diethylaminoethyl
T70177 950-966 CHEBI:63050;CHEBI:63050 denotes ammonium formate
T31019 986-991 CHEBI:15377;CHEBI:15377 denotes water
T60694 1006-1013 CHEBI:16236;CHEBI:16236 denotes ethanol
T18203 1076-1078 CHEBI:29238;CHEBI:29238 denotes 3H
T42093 1394-1402 CHEBI:36357;CHEBI:36357 denotes compound

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T107 52-55 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T108 344-347 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T109 432-435 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T110 568-571 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T111 902-905 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T112 1086-1105 Body_part denotes transfer to the RNA http://purl.org/sig/ont/fma/fma67127
T113 1102-1105 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
1618 39-41 Chemical denotes 3H MESH:D014316
1619 217-221 Chemical denotes Tris
1620 222-225 Chemical denotes HCl MESH:D006851
1621 241-244 Chemical denotes DTT MESH:D004229
1622 251-256 Chemical denotes MgCl2 MESH:D015636
1623 278-280 Chemical denotes 3H MESH:D014316
1624 524-528 Chemical denotes DMSO MESH:D004121
1625 539-543 Chemical denotes DMSO MESH:D004121
1626 674-678 Chemical denotes DMSO MESH:D004121
1627 763-768 Chemical denotes water MESH:D014867
1628 798-815 Chemical denotes diethylaminoethyl
1629 817-821 Chemical denotes DEAE
1630 950-966 Chemical denotes ammonium formate MESH:C030544
1631 986-991 Chemical denotes water MESH:D014867
1632 1006-1013 Chemical denotes ethanol MESH:D000431
1633 1076-1078 Chemical denotes 3H MESH:D014316

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T102 352-360 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T103 376-384 Disease denotes vaccinia http://purl.obolibrary.org/obo/MONDO_0002595

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T445 385-390 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T446 416-418 http://purl.obolibrary.org/obo/CLO_0053794 denotes 41
T447 426-431 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T448 853-854 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T449 884-895 http://purl.obolibrary.org/obo/OBI_0000968 denotes Instruments
T450 1138-1139 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T451 1453-1461 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T950 26-32 Chemical denotes methyl http://purl.obolibrary.org/obo/CHEBI_32875|http://purl.obolibrary.org/obo/CHEBI_29309
T952 43-46 Chemical denotes SAM http://purl.obolibrary.org/obo/CHEBI_15414|http://purl.obolibrary.org/obo/CHEBI_67040
T954 202-209 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004
T955 217-221 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T956 222-225 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T957 241-244 Chemical denotes DTT http://purl.obolibrary.org/obo/CHEBI_18320
T958 251-256 Chemical denotes MgCl2 http://purl.obolibrary.org/obo/CHEBI_6636
T959 263-266 Chemical denotes SAM http://purl.obolibrary.org/obo/CHEBI_15414|http://purl.obolibrary.org/obo/CHEBI_67040
T961 281-284 Chemical denotes SAM http://purl.obolibrary.org/obo/CHEBI_15414|http://purl.obolibrary.org/obo/CHEBI_67040
T963 524-528 Chemical denotes DMSO http://purl.obolibrary.org/obo/CHEBI_28262
T964 539-543 Chemical denotes DMSO http://purl.obolibrary.org/obo/CHEBI_28262
T965 586-589 Chemical denotes SAM http://purl.obolibrary.org/obo/CHEBI_15414|http://purl.obolibrary.org/obo/CHEBI_67040
T967 674-678 Chemical denotes DMSO http://purl.obolibrary.org/obo/CHEBI_28262
T968 763-768 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377
T969 817-821 Chemical denotes DEAE http://purl.obolibrary.org/obo/CHEBI_52153
T970 950-966 Chemical denotes ammonium formate http://purl.obolibrary.org/obo/CHEBI_63050
T971 950-958 Chemical denotes ammonium http://purl.obolibrary.org/obo/CHEBI_28938
T972 959-966 Chemical denotes formate http://purl.obolibrary.org/obo/CHEBI_15740|http://purl.obolibrary.org/obo/CHEBI_52343
T974 986-991 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377
T975 1006-1013 Chemical denotes ethanol http://purl.obolibrary.org/obo/CHEBI_16236
T976 1079-1085 Chemical denotes methyl http://purl.obolibrary.org/obo/CHEBI_32875|http://purl.obolibrary.org/obo/CHEBI_29309
T978 1185-1192 Chemical denotes Counter http://purl.obolibrary.org/obo/CHEBI_38960

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T51 1086-1105 http://purl.obolibrary.org/obo/GO_0030533 denotes transfer to the RNA

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T563 0-165 Sentence denotes The transfer of tritiated methyl from [3H] SAM onto RNA substrate was monitored by filter binding assay, performed according to the method described previously [40].
T564 166-461 Sentence denotes Assays were carried out in reaction mixture [40 mM Tris-HCl (pH 8.0), 1 mM DTT, 1 mM MgCl2, 2 μM SAM and 0.1 μM 3H-SAM (PerkinElmer)] in the presence of 0.7 μM GpppAC4 synthetic RNA and SARS-CoV nsp14 (50 nM), vaccinia virus capping enzyme (D1-D12) (41 U), or human RNA N7 MTase (hRNMT) (50 nM).
T565 462-618 Sentence denotes The enzymes were first mixed with compounds suspended in 100% DMSO (5% final DMSO) before the addition of RNA substrate and SAM and then incubated at 30 °C.
T566 619-679 Sentence denotes Control reactions were performed in the presence of 5% DMSO.
T567 680-769 Sentence denotes Reactions mixtures were stopped after 30 min by their 10-fold dilution in ice-cold water.
T568 770-897 Sentence denotes Samples were transferred to diethylaminoethyl (DEAE) filtermat (PerkinElmer) using a Filtermat Harvester (Packard Instruments).
T569 898-1014 Sentence denotes The RNA-retaining mats were washed twice with 10 mM ammonium formate pH 8.0, twice with water and once with ethanol.
T570 1015-1207 Sentence denotes They were soaked with scintillation fluid (PerkinElmer), and 3H-methyl transfer to the RNA substrates was determined using a Wallac MicroBeta TriLux Liquid Scintillation Counter (PerkinElmer).
T571 1208-1359 Sentence denotes For IC50 measurements, values were normalized and fitted with Prism (GraphPad software) using the following equation: Y = 100/(1+((X/IC50)^Hillslope)).
T572 1360-1462 Sentence denotes IC50 is defined as the inhibitory compound concentration that causes 50% reduction in enzyme activity.