PMC:7291971 / 34260-35429 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T720","span":{"begin":34,"end":42},"obj":"CHEBI:46787;CHEBI:46787"},{"id":"T721","span":{"begin":47,"end":55},"obj":"CHEBI:33893;CHEBI:33893"},{"id":"T722","span":{"begin":165,"end":172},"obj":"CHEBI:29320;CHEBI:29320"},{"id":"T723","span":{"begin":173,"end":180},"obj":"CHEBI:29239;CHEBI:29239"},{"id":"T724","span":{"begin":243,"end":249},"obj":"CHEBI:30563;CHEBI:30563"},{"id":"T725","span":{"begin":911,"end":918},"obj":"CHEBI:46787;CHEBI:46787"},{"id":"T726","span":{"begin":980,"end":985},"obj":"CHEBI:1418;CHEBI:1418"},{"id":"T727","span":{"begin":1089,"end":1098},"obj":"CHEBI:36357;CHEBI:36357"},{"id":"T86649","span":{"begin":34,"end":42},"obj":"CHEBI:46787;CHEBI:46787"},{"id":"T43192","span":{"begin":47,"end":55},"obj":"CHEBI:33893;CHEBI:33893"},{"id":"T3261","span":{"begin":165,"end":172},"obj":"CHEBI:29320;CHEBI:29320"},{"id":"T10589","span":{"begin":173,"end":180},"obj":"CHEBI:29239;CHEBI:29239"},{"id":"T99839","span":{"begin":243,"end":249},"obj":"CHEBI:30563;CHEBI:30563"},{"id":"T52140","span":{"begin":911,"end":918},"obj":"CHEBI:46787;CHEBI:46787"},{"id":"T30031","span":{"begin":980,"end":985},"obj":"CHEBI:1418;CHEBI:1418"},{"id":"T39091","span":{"begin":1089,"end":1098},"obj":"CHEBI:36357;CHEBI:36357"}],"text":"4.1.1 General procedures\nAll dry solvents and reagents were purchased from commercial suppliers and were used without further purification. DIEA was distilled over calcium hydride. Thin-layer chromatography (TLC) analyses were carried out on silica plate 60 F254. Purifications by column chromatography were performed using Biotage Isolera 1 system with FlashPure cartridges (Buchi). NMR experiments were recorded on Bruker 400, 500 or 600 MHz spectrometers at 20 °C. HRMS analyses were obtained with electrospray ionization (ESI) in positive mode on a Q-TOF Micromass spectrometer. Analytical HPLC was performed on a UHPLC Thermoscientific Ultimate 3000 system equipped with a LPG-3400RS pump, a DAD 3000 detector and an WPS-3000TBRS Autosampler, Column Oven TCC-3000SD. Dinucleosides 1–16 were analyzed by RP-HPLC on a Column Nucleodur C18 ec 100-3, 4.6 × 75 mm (Macherey Nagel) at 30 °C. The following HPLC solvent systems were used: 1% CH3CN in 12.5 mM TEAAc (buffer A), 80% CH3CN in 12.5 mM TEEAc (buffer B). Flow rate was 1 mL/min. UV detection was performed at 260 nm. Lyophilized compounds 1–16 were stored at −20 °C for several months without any degradation."}

{"project":"LitCovid-PubTator","denotations":[{"id":"561","span":{"begin":141,"end":145},"obj":"Chemical"},{"id":"562","span":{"begin":165,"end":180},"obj":"Chemical"},{"id":"563","span":{"begin":243,"end":249},"obj":"Chemical"},{"id":"564","span":{"begin":773,"end":786},"obj":"Chemical"},{"id":"565","span":{"begin":941,"end":946},"obj":"Chemical"},{"id":"566","span":{"begin":958,"end":963},"obj":"Chemical"},{"id":"567","span":{"begin":980,"end":985},"obj":"Chemical"},{"id":"568","span":{"begin":997,"end":1002},"obj":"Chemical"},{"id":"569","span":{"begin":469,"end":473},"obj":"Disease"}],"attributes":[{"id":"A563","pred":"tao:has_database_id","subj":"563","obj":"MESH:D012822"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"4.1.1 General procedures\nAll dry solvents and reagents were purchased from commercial suppliers and were used without further purification. DIEA was distilled over calcium hydride. Thin-layer chromatography (TLC) analyses were carried out on silica plate 60 F254. Purifications by column chromatography were performed using Biotage Isolera 1 system with FlashPure cartridges (Buchi). NMR experiments were recorded on Bruker 400, 500 or 600 MHz spectrometers at 20 °C. HRMS analyses were obtained with electrospray ionization (ESI) in positive mode on a Q-TOF Micromass spectrometer. Analytical HPLC was performed on a UHPLC Thermoscientific Ultimate 3000 system equipped with a LPG-3400RS pump, a DAD 3000 detector and an WPS-3000TBRS Autosampler, Column Oven TCC-3000SD. Dinucleosides 1–16 were analyzed by RP-HPLC on a Column Nucleodur C18 ec 100-3, 4.6 × 75 mm (Macherey Nagel) at 30 °C. The following HPLC solvent systems were used: 1% CH3CN in 12.5 mM TEAAc (buffer A), 80% CH3CN in 12.5 mM TEEAc (buffer B). Flow rate was 1 mL/min. UV detection was performed at 260 nm. Lyophilized compounds 1–16 were stored at −20 °C for several months without any degradation."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T98","span":{"begin":679,"end":682},"obj":"Disease"},{"id":"T99","span":{"begin":761,"end":764},"obj":"Disease"}],"attributes":[{"id":"A98","pred":"mondo_id","subj":"T98","obj":"http://purl.obolibrary.org/obo/MONDO_0012725"},{"id":"A99","pred":"mondo_id","subj":"T99","obj":"http://purl.obolibrary.org/obo/MONDO_0008521"}],"text":"4.1.1 General procedures\nAll dry solvents and reagents were purchased from commercial suppliers and were used without further purification. DIEA was distilled over calcium hydride. Thin-layer chromatography (TLC) analyses were carried out on silica plate 60 F254. Purifications by column chromatography were performed using Biotage Isolera 1 system with FlashPure cartridges (Buchi). NMR experiments were recorded on Bruker 400, 500 or 600 MHz spectrometers at 20 °C. HRMS analyses were obtained with electrospray ionization (ESI) in positive mode on a Q-TOF Micromass spectrometer. Analytical HPLC was performed on a UHPLC Thermoscientific Ultimate 3000 system equipped with a LPG-3400RS pump, a DAD 3000 detector and an WPS-3000TBRS Autosampler, Column Oven TCC-3000SD. Dinucleosides 1–16 were analyzed by RP-HPLC on a Column Nucleodur C18 ec 100-3, 4.6 × 75 mm (Macherey Nagel) at 30 °C. The following HPLC solvent systems were used: 1% CH3CN in 12.5 mM TEAAc (buffer A), 80% CH3CN in 12.5 mM TEEAc (buffer B). Flow rate was 1 mL/min. UV detection was performed at 260 nm. Lyophilized compounds 1–16 were stored at −20 °C for several months without any degradation."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T255","span":{"begin":552,"end":553},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T256","span":{"begin":617,"end":618},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T257","span":{"begin":677,"end":678},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T258","span":{"begin":696,"end":697},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T259","span":{"begin":787,"end":791},"obj":"http://purl.obolibrary.org/obo/CLO_0001046"},{"id":"T260","span":{"begin":820,"end":821},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T261","span":{"begin":843,"end":845},"obj":"http://purl.obolibrary.org/obo/CLO_0002878"},{"id":"T262","span":{"begin":972,"end":973},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T263","span":{"begin":1011,"end":1012},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T264","span":{"begin":1099,"end":1103},"obj":"http://purl.obolibrary.org/obo/CLO_0001046"}],"text":"4.1.1 General procedures\nAll dry solvents and reagents were purchased from commercial suppliers and were used without further purification. DIEA was distilled over calcium hydride. Thin-layer chromatography (TLC) analyses were carried out on silica plate 60 F254. Purifications by column chromatography were performed using Biotage Isolera 1 system with FlashPure cartridges (Buchi). NMR experiments were recorded on Bruker 400, 500 or 600 MHz spectrometers at 20 °C. HRMS analyses were obtained with electrospray ionization (ESI) in positive mode on a Q-TOF Micromass spectrometer. Analytical HPLC was performed on a UHPLC Thermoscientific Ultimate 3000 system equipped with a LPG-3400RS pump, a DAD 3000 detector and an WPS-3000TBRS Autosampler, Column Oven TCC-3000SD. Dinucleosides 1–16 were analyzed by RP-HPLC on a Column Nucleodur C18 ec 100-3, 4.6 × 75 mm (Macherey Nagel) at 30 °C. The following HPLC solvent systems were used: 1% CH3CN in 12.5 mM TEAAc (buffer A), 80% CH3CN in 12.5 mM TEEAc (buffer B). Flow rate was 1 mL/min. UV detection was performed at 260 nm. Lyophilized compounds 1–16 were stored at −20 °C for several months without any degradation."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T564","span":{"begin":34,"end":42},"obj":"Chemical"},{"id":"T565","span":{"begin":47,"end":55},"obj":"Chemical"},{"id":"T566","span":{"begin":165,"end":172},"obj":"Chemical"},{"id":"T568","span":{"begin":173,"end":180},"obj":"Chemical"},{"id":"T569","span":{"begin":243,"end":249},"obj":"Chemical"},{"id":"T570","span":{"begin":679,"end":682},"obj":"Chemical"},{"id":"T571","span":{"begin":761,"end":764},"obj":"Chemical"},{"id":"T572","span":{"begin":809,"end":811},"obj":"Chemical"},{"id":"T573","span":{"begin":911,"end":918},"obj":"Chemical"},{"id":"T574","span":{"begin":965,"end":971},"obj":"Chemical"},{"id":"T575","span":{"begin":1004,"end":1010},"obj":"Chemical"}],"attributes":[{"id":"A564","pred":"chebi_id","subj":"T564","obj":"http://purl.obolibrary.org/obo/CHEBI_46787"},{"id":"A565","pred":"chebi_id","subj":"T565","obj":"http://purl.obolibrary.org/obo/CHEBI_33893"},{"id":"A566","pred":"chebi_id","subj":"T566","obj":"http://purl.obolibrary.org/obo/CHEBI_22984"},{"id":"A567","pred":"chebi_id","subj":"T566","obj":"http://purl.obolibrary.org/obo/CHEBI_29320"},{"id":"A568","pred":"chebi_id","subj":"T568","obj":"http://purl.obolibrary.org/obo/CHEBI_29239"},{"id":"A569","pred":"chebi_id","subj":"T569","obj":"http://purl.obolibrary.org/obo/CHEBI_30563"},{"id":"A570","pred":"chebi_id","subj":"T570","obj":"http://purl.obolibrary.org/obo/CHEBI_59681"},{"id":"A571","pred":"chebi_id","subj":"T571","obj":"http://purl.obolibrary.org/obo/CHEBI_48347"},{"id":"A572","pred":"chebi_id","subj":"T572","obj":"http://purl.obolibrary.org/obo/CHEBI_141419"},{"id":"A573","pred":"chebi_id","subj":"T573","obj":"http://purl.obolibrary.org/obo/CHEBI_46787"},{"id":"A574","pred":"chebi_id","subj":"T574","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A575","pred":"chebi_id","subj":"T575","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"}],"text":"4.1.1 General procedures\nAll dry solvents and reagents were purchased from commercial suppliers and were used without further purification. DIEA was distilled over calcium hydride. Thin-layer chromatography (TLC) analyses were carried out on silica plate 60 F254. Purifications by column chromatography were performed using Biotage Isolera 1 system with FlashPure cartridges (Buchi). NMR experiments were recorded on Bruker 400, 500 or 600 MHz spectrometers at 20 °C. HRMS analyses were obtained with electrospray ionization (ESI) in positive mode on a Q-TOF Micromass spectrometer. Analytical HPLC was performed on a UHPLC Thermoscientific Ultimate 3000 system equipped with a LPG-3400RS pump, a DAD 3000 detector and an WPS-3000TBRS Autosampler, Column Oven TCC-3000SD. Dinucleosides 1–16 were analyzed by RP-HPLC on a Column Nucleodur C18 ec 100-3, 4.6 × 75 mm (Macherey Nagel) at 30 °C. The following HPLC solvent systems were used: 1% CH3CN in 12.5 mM TEAAc (buffer A), 80% CH3CN in 12.5 mM TEEAc (buffer B). Flow rate was 1 mL/min. UV detection was performed at 260 nm. Lyophilized compounds 1–16 were stored at −20 °C for several months without any degradation."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T45","span":{"begin":1157,"end":1168},"obj":"http://purl.obolibrary.org/obo/GO_0009056"}],"text":"4.1.1 General procedures\nAll dry solvents and reagents were purchased from commercial suppliers and were used without further purification. DIEA was distilled over calcium hydride. Thin-layer chromatography (TLC) analyses were carried out on silica plate 60 F254. Purifications by column chromatography were performed using Biotage Isolera 1 system with FlashPure cartridges (Buchi). NMR experiments were recorded on Bruker 400, 500 or 600 MHz spectrometers at 20 °C. HRMS analyses were obtained with electrospray ionization (ESI) in positive mode on a Q-TOF Micromass spectrometer. Analytical HPLC was performed on a UHPLC Thermoscientific Ultimate 3000 system equipped with a LPG-3400RS pump, a DAD 3000 detector and an WPS-3000TBRS Autosampler, Column Oven TCC-3000SD. Dinucleosides 1–16 were analyzed by RP-HPLC on a Column Nucleodur C18 ec 100-3, 4.6 × 75 mm (Macherey Nagel) at 30 °C. The following HPLC solvent systems were used: 1% CH3CN in 12.5 mM TEAAc (buffer A), 80% CH3CN in 12.5 mM TEEAc (buffer B). Flow rate was 1 mL/min. UV detection was performed at 260 nm. Lyophilized compounds 1–16 were stored at −20 °C for several months without any degradation."}

{"project":"LitCovid-sentences","denotations":[{"id":"T246","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T247","span":{"begin":26,"end":140},"obj":"Sentence"},{"id":"T248","span":{"begin":141,"end":181},"obj":"Sentence"},{"id":"T249","span":{"begin":182,"end":264},"obj":"Sentence"},{"id":"T250","span":{"begin":265,"end":384},"obj":"Sentence"},{"id":"T251","span":{"begin":385,"end":468},"obj":"Sentence"},{"id":"T252","span":{"begin":469,"end":583},"obj":"Sentence"},{"id":"T253","span":{"begin":584,"end":772},"obj":"Sentence"},{"id":"T254","span":{"begin":773,"end":891},"obj":"Sentence"},{"id":"T255","span":{"begin":892,"end":937},"obj":"Sentence"},{"id":"T256","span":{"begin":938,"end":1014},"obj":"Sentence"},{"id":"T257","span":{"begin":1015,"end":1038},"obj":"Sentence"},{"id":"T258","span":{"begin":1039,"end":1076},"obj":"Sentence"},{"id":"T259","span":{"begin":1077,"end":1169},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"4.1.1 General procedures\nAll dry solvents and reagents were purchased from commercial suppliers and were used without further purification. DIEA was distilled over calcium hydride. Thin-layer chromatography (TLC) analyses were carried out on silica plate 60 F254. Purifications by column chromatography were performed using Biotage Isolera 1 system with FlashPure cartridges (Buchi). NMR experiments were recorded on Bruker 400, 500 or 600 MHz spectrometers at 20 °C. HRMS analyses were obtained with electrospray ionization (ESI) in positive mode on a Q-TOF Micromass spectrometer. Analytical HPLC was performed on a UHPLC Thermoscientific Ultimate 3000 system equipped with a LPG-3400RS pump, a DAD 3000 detector and an WPS-3000TBRS Autosampler, Column Oven TCC-3000SD. Dinucleosides 1–16 were analyzed by RP-HPLC on a Column Nucleodur C18 ec 100-3, 4.6 × 75 mm (Macherey Nagel) at 30 °C. The following HPLC solvent systems were used: 1% CH3CN in 12.5 mM TEAAc (buffer A), 80% CH3CN in 12.5 mM TEEAc (buffer B). Flow rate was 1 mL/min. UV detection was performed at 260 nm. Lyophilized compounds 1–16 were stored at −20 °C for several months without any degradation."}