PMC:7281900 / 721-1194 JSONTXT

{"project":"testtesttest","denotations":[{"id":"T2","span":{"begin":45,"end":67},"obj":"Body_part"},{"id":"T3","span":{"begin":352,"end":368},"obj":"Body_part"}],"attributes":[{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL_0002248"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0011026"}],"text":"Here, we generated patient-specific, induced pluripotent stem cells (iPSCs) from a patient with TSC with a heterozygous, germline, nonsense mutation in exon 15 of TSC1 and established an isogenic set of heterozygous (Het), null, and corrected wild-type (Corr-WT) iPSCs using CRISPR/Cas9-mediated gene editing. We differentiated these iPSCs into neural progenitor cells (NPCs) and examined neurodevelopmental phenotypes, signaling, and changes in gene expression by RNA-seq."}