Here, we generated patient-specific, induced pluripotent stem cells (iPSCs) from a patient with TSC with a heterozygous, germline, nonsense mutation in exon 15 of TSC1 and established an isogenic set of heterozygous (Het), null, and corrected wild-type (Corr-WT) iPSCs using CRISPR/Cas9-mediated gene editing. We differentiated these iPSCs into neural progenitor cells (NPCs) and examined neurodevelopmental phenotypes, signaling, and changes in gene expression by RNA-seq.