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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7272971","sourcedb":"PMC","sourceid":"7272971","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7272971","text":"Expression of the gene for ACE2 has been shown in corneal and conjunctival epithelial cells, and preliminary evidence has been presented for the presence of the protein associated with these cells. 40 The isolated surface protein S240 of SARS‐CoV can bind to conjunctival epithelial and fibroblast cells and corneal epithelial cells, with this binding inhibited by soluble ACE2, indicating that the binding was being mediated through cell‐surface ACE2. 31 However, the authors did note that ‘the amount of ACE2 expressed in conjunctival and corneal cells and tissues (was) less than that in heart, lung tissues and Vero E6 cells’, and was approximately 50 per cent that of Vero E6 cells. 31 Another paper provided evidence of expression of mRNA for ACE2 in corneal epithelium with no evidence provided of mRNA from the conjunctiva. 41 The normalised expression of ACE2 in the corneal epithelial mRNA was only 20‐28 units, compared to \u003e 400 units in the testes, \u003e 200 units in the small intestine terminal ileum and \u003e 100 units in the heart left ventricle. 41 The previously mentioned serine protease TMPRSS2 or Furin, which appear to prime the spike protein, had not been reported in tears or on corneal or conjunctival cells. However, 15 per cent of superficial conjunctival cells have been shown to have “greater‐than‐zero expression” of the gene for TMPRSS2, three per cent of cells in superficial conjunctiva and one per cent of limbal superficial cells have “greater‐than‐zero expression” of genes of both TMPRSS2 and ACE2. The mean level of expression of mRNA for ACE2 or TMPRSS2 in any ocular tissue was approximately 0.6 per cent, whereas the mean level of expression was greater (1.2–1.4 per cent) in most other tissues (such as nasal cavity, lung parenchyma, ileum, colon, heart) examined. 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