PMC:7258756 / 7141-7743 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"137","span":{"begin":307,"end":311},"obj":"Gene"},{"id":"138","span":{"begin":317,"end":321},"obj":"Gene"},{"id":"139","span":{"begin":216,"end":226},"obj":"Species"}],"attributes":[{"id":"A137","pred":"tao:has_database_id","subj":"137","obj":"Gene:43740578"},{"id":"A138","pred":"tao:has_database_id","subj":"138","obj":"Gene:43740578"},{"id":"A139","pred":"tao:has_database_id","subj":"139","obj":"Tax:2697049"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The viral RNA was extracted from the TS sample using the Magmax RNA extraction kit (Applied Biosystems, USA) as per the manufacturer's instructions. The extracted RNA was immediately used for testing the presence of SARS-CoV-2 using the real-time RT-PCR protocol published by the WHO12 for the detection of RdRp (1), RdRp (2), E gene and N gene. RNase P gene was used as the internal control for the analysis. Confirmatory laboratory tests were performed as per the WHO-recommended test protocols13. These samples were also sequenced using the NGS approach to retrieve the complete genome of the virus."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T26","span":{"begin":10,"end":13},"obj":"Body_part"},{"id":"T27","span":{"begin":64,"end":67},"obj":"Body_part"},{"id":"T28","span":{"begin":163,"end":166},"obj":"Body_part"},{"id":"T29","span":{"begin":329,"end":333},"obj":"Body_part"},{"id":"T30","span":{"begin":340,"end":344},"obj":"Body_part"},{"id":"T31","span":{"begin":354,"end":358},"obj":"Body_part"},{"id":"T32","span":{"begin":582,"end":588},"obj":"Body_part"}],"attributes":[{"id":"A26","pred":"fma_id","subj":"T26","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A27","pred":"fma_id","subj":"T27","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A28","pred":"fma_id","subj":"T28","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A29","pred":"fma_id","subj":"T29","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A30","pred":"fma_id","subj":"T30","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A31","pred":"fma_id","subj":"T31","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A32","pred":"fma_id","subj":"T32","obj":"http://purl.org/sig/ont/fma/fma84116"}],"text":"The viral RNA was extracted from the TS sample using the Magmax RNA extraction kit (Applied Biosystems, USA) as per the manufacturer's instructions. The extracted RNA was immediately used for testing the presence of SARS-CoV-2 using the real-time RT-PCR protocol published by the WHO12 for the detection of RdRp (1), RdRp (2), E gene and N gene. RNase P gene was used as the internal control for the analysis. Confirmatory laboratory tests were performed as per the WHO-recommended test protocols13. These samples were also sequenced using the NGS approach to retrieve the complete genome of the virus."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T69","span":{"begin":37,"end":39},"obj":"Disease"},{"id":"T71","span":{"begin":216,"end":224},"obj":"Disease"},{"id":"T72","span":{"begin":216,"end":220},"obj":"Disease"}],"attributes":[{"id":"A69","pred":"mondo_id","subj":"T69","obj":"http://purl.obolibrary.org/obo/MONDO_0010979"},{"id":"A70","pred":"mondo_id","subj":"T69","obj":"http://purl.obolibrary.org/obo/MONDO_0016455"},{"id":"A71","pred":"mondo_id","subj":"T71","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A72","pred":"mondo_id","subj":"T72","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"The viral RNA was extracted from the TS sample using the Magmax RNA extraction kit (Applied Biosystems, USA) as per the manufacturer's instructions. The extracted RNA was immediately used for testing the presence of SARS-CoV-2 using the real-time RT-PCR protocol published by the WHO12 for the detection of RdRp (1), RdRp (2), E gene and N gene. RNase P gene was used as the internal control for the analysis. Confirmatory laboratory tests were performed as per the WHO-recommended test protocols13. These samples were also sequenced using the NGS approach to retrieve the complete genome of the virus."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T61","span":{"begin":192,"end":199},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T62","span":{"begin":329,"end":333},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T63","span":{"begin":340,"end":344},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T64","span":{"begin":354,"end":358},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T65","span":{"begin":434,"end":439},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T66","span":{"begin":482,"end":486},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T67","span":{"begin":596,"end":601},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"}],"text":"The viral RNA was extracted from the TS sample using the Magmax RNA extraction kit (Applied Biosystems, USA) as per the manufacturer's instructions. The extracted RNA was immediately used for testing the presence of SARS-CoV-2 using the real-time RT-PCR protocol published by the WHO12 for the detection of RdRp (1), RdRp (2), E gene and N gene. RNase P gene was used as the internal control for the analysis. Confirmatory laboratory tests were performed as per the WHO-recommended test protocols13. These samples were also sequenced using the NGS approach to retrieve the complete genome of the virus."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T16","span":{"begin":37,"end":39},"obj":"Chemical"}],"attributes":[{"id":"A16","pred":"chebi_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/CHEBI_73664"}],"text":"The viral RNA was extracted from the TS sample using the Magmax RNA extraction kit (Applied Biosystems, USA) as per the manufacturer's instructions. The extracted RNA was immediately used for testing the presence of SARS-CoV-2 using the real-time RT-PCR protocol published by the WHO12 for the detection of RdRp (1), RdRp (2), E gene and N gene. RNase P gene was used as the internal control for the analysis. Confirmatory laboratory tests were performed as per the WHO-recommended test protocols13. These samples were also sequenced using the NGS approach to retrieve the complete genome of the virus."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T9","span":{"begin":346,"end":353},"obj":"http://purl.obolibrary.org/obo/GO_0004526"}],"text":"The viral RNA was extracted from the TS sample using the Magmax RNA extraction kit (Applied Biosystems, USA) as per the manufacturer's instructions. The extracted RNA was immediately used for testing the presence of SARS-CoV-2 using the real-time RT-PCR protocol published by the WHO12 for the detection of RdRp (1), RdRp (2), E gene and N gene. RNase P gene was used as the internal control for the analysis. Confirmatory laboratory tests were performed as per the WHO-recommended test protocols13. These samples were also sequenced using the NGS approach to retrieve the complete genome of the virus."}

{"project":"LitCovid-sentences","denotations":[{"id":"T56","span":{"begin":0,"end":148},"obj":"Sentence"},{"id":"T57","span":{"begin":149,"end":345},"obj":"Sentence"},{"id":"T58","span":{"begin":346,"end":409},"obj":"Sentence"},{"id":"T59","span":{"begin":410,"end":499},"obj":"Sentence"},{"id":"T60","span":{"begin":500,"end":602},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The viral RNA was extracted from the TS sample using the Magmax RNA extraction kit (Applied Biosystems, USA) as per the manufacturer's instructions. The extracted RNA was immediately used for testing the presence of SARS-CoV-2 using the real-time RT-PCR protocol published by the WHO12 for the detection of RdRp (1), RdRp (2), E gene and N gene. RNase P gene was used as the internal control for the analysis. Confirmatory laboratory tests were performed as per the WHO-recommended test protocols13. These samples were also sequenced using the NGS approach to retrieve the complete genome of the virus."}