PMC:7253482 / 28321-29088 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7253482","sourcedb":"PMC","sourceid":"7253482","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7253482","text":"Labelled glycans were analysed using a 2.1 mm × 150 mm Acquity BEH Glycan column (Waters) on an Acquity H-Class UPLC instrument (Waters), with fluorescence measurements occurring at λex = 310 nm and λem = 370 nm. The following gradient was used: time (t) = 0: 22% A, 78% B (flow rate = 0.5 mL/min); t = 38.5: 44.1% A, 55.9% B (0.5 mL/min); t = 39.5: 100% A, 0% B (0.25 mL/min); t = 44.5: 100% A, 0% B (0.25 mL/min); t = 46.5: 22% A, 78% B (0.5 mL/min), where solvent A was 50 mM ammonium formate (pH 4.4) and B was acetonitrile. Quantification of oligomannose-type glycans was achieved by digestion of fluorescently labelled glycans with Endo H, and clean-up using a PVDF protein-binding membrane (Millipore). Empower 3 software (Waters) was used for data processing.","tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"910","span":{"begin":9,"end":16},"obj":"Chemical"},{"id":"911","span":{"begin":63,"end":73},"obj":"Chemical"},{"id":"912","span":{"begin":547,"end":559},"obj":"Chemical"},{"id":"913","span":{"begin":565,"end":572},"obj":"Chemical"},{"id":"914","span":{"begin":625,"end":632},"obj":"Chemical"},{"id":"915","span":{"begin":667,"end":671},"obj":"Chemical"}],"attributes":[{"id":"A910","pred":"tao:has_database_id","subj":"910","obj":"MESH:D011134"},{"id":"A913","pred":"tao:has_database_id","subj":"913","obj":"MESH:D011134"},{"id":"A914","pred":"tao:has_database_id","subj":"914","obj":"MESH:D011134"},{"id":"A915","pred":"tao:has_database_id","subj":"915","obj":"MESH:C024865"},{"subj":"910","pred":"source","obj":"LitCovid-PubTator"},{"subj":"911","pred":"source","obj":"LitCovid-PubTator"},{"subj":"912","pred":"source","obj":"LitCovid-PubTator"},{"subj":"913","pred":"source","obj":"LitCovid-PubTator"},{"subj":"914","pred":"source","obj":"LitCovid-PubTator"},{"subj":"915","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T155","span":{"begin":672,"end":679},"obj":"Body_part"}],"attributes":[{"id":"A155","pred":"fma_id","subj":"T155","obj":"http://purl.org/sig/ont/fma/fma67257"},{"subj":"T155","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T189","span":{"begin":0,"end":8},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"},{"id":"T190","span":{"begin":37,"end":38},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T191","span":{"begin":117,"end":127},"obj":"http://purl.obolibrary.org/obo/OBI_0000968"},{"id":"T192","span":{"begin":260,"end":262},"obj":"http://purl.obolibrary.org/obo/CLO_0050507"},{"id":"T193","span":{"begin":264,"end":265},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T194","span":{"begin":271,"end":272},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T195","span":{"begin":315,"end":316},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T196","span":{"begin":324,"end":325},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T197","span":{"begin":355,"end":356},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T198","span":{"begin":361,"end":362},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T199","span":{"begin":393,"end":394},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T200","span":{"begin":399,"end":400},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T201","span":{"begin":426,"end":428},"obj":"http://purl.obolibrary.org/obo/CLO_0050507"},{"id":"T202","span":{"begin":430,"end":431},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T203","span":{"begin":437,"end":438},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T204","span":{"begin":467,"end":468},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T205","span":{"begin":509,"end":510},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T206","span":{"begin":616,"end":624},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"},{"id":"T207","span":{"begin":665,"end":666},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T208","span":{"begin":688,"end":696},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"}],"attributes":[{"subj":"T189","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T190","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T191","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T192","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T193","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T194","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T195","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T196","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T197","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T198","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T199","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T200","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T201","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T202","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T203","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T204","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T205","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T206","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T207","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T208","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T279","span":{"begin":9,"end":16},"obj":"Chemical"},{"id":"T280","span":{"begin":67,"end":73},"obj":"Chemical"},{"id":"T281","span":{"begin":459,"end":466},"obj":"Chemical"},{"id":"T282","span":{"begin":479,"end":495},"obj":"Chemical"},{"id":"T283","span":{"begin":479,"end":487},"obj":"Chemical"},{"id":"T284","span":{"begin":488,"end":495},"obj":"Chemical"},{"id":"T286","span":{"begin":515,"end":527},"obj":"Chemical"},{"id":"T287","span":{"begin":565,"end":572},"obj":"Chemical"},{"id":"T288","span":{"begin":625,"end":632},"obj":"Chemical"},{"id":"T289","span":{"begin":672,"end":679},"obj":"Chemical"}],"attributes":[{"id":"A279","pred":"chebi_id","subj":"T279","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A280","pred":"chebi_id","subj":"T280","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A281","pred":"chebi_id","subj":"T281","obj":"http://purl.obolibrary.org/obo/CHEBI_46787"},{"id":"A282","pred":"chebi_id","subj":"T282","obj":"http://purl.obolibrary.org/obo/CHEBI_63050"},{"id":"A283","pred":"chebi_id","subj":"T283","obj":"http://purl.obolibrary.org/obo/CHEBI_28938"},{"id":"A284","pred":"chebi_id","subj":"T284","obj":"http://purl.obolibrary.org/obo/CHEBI_15740"},{"id":"A285","pred":"chebi_id","subj":"T284","obj":"http://purl.obolibrary.org/obo/CHEBI_52343"},{"id":"A286","pred":"chebi_id","subj":"T286","obj":"http://purl.obolibrary.org/obo/CHEBI_38472"},{"id":"A287","pred":"chebi_id","subj":"T287","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A288","pred":"chebi_id","subj":"T288","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A289","pred":"chebi_id","subj":"T289","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"subj":"T279","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T280","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T281","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T282","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T283","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T284","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T286","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T287","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T288","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T289","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-sample-PD-FMA","denotations":[{"id":"T154","span":{"begin":672,"end":679},"obj":"Body_part"}],"attributes":[{"id":"A154","pred":"fma_id","subj":"T154","obj":"http://purl.org/sig/ont/fma/fma67257"},{"subj":"T154","pred":"source","obj":"LitCovid-sample-PD-FMA"}]},{"project":"LitCovid-sample-CHEBI","denotations":[{"id":"T192","span":{"begin":9,"end":16},"obj":"Chemical"},{"id":"T193","span":{"begin":67,"end":73},"obj":"Chemical"},{"id":"T194","span":{"begin":479,"end":495},"obj":"Chemical"},{"id":"T195","span":{"begin":515,"end":527},"obj":"Chemical"},{"id":"T196","span":{"begin":565,"end":572},"obj":"Chemical"},{"id":"T197","span":{"begin":625,"end":632},"obj":"Chemical"},{"id":"T198","span":{"begin":672,"end":679},"obj":"Chemical"}],"attributes":[{"id":"A192","pred":"chebi_id","subj":"T192","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A193","pred":"chebi_id","subj":"T193","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A194","pred":"chebi_id","subj":"T194","obj":"http://purl.obolibrary.org/obo/CHEBI_63050"},{"id":"A195","pred":"chebi_id","subj":"T195","obj":"http://purl.obolibrary.org/obo/CHEBI_38472"},{"id":"A196","pred":"chebi_id","subj":"T196","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A197","pred":"chebi_id","subj":"T197","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A198","pred":"chebi_id","subj":"T198","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"subj":"T192","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T193","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T194","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T195","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T196","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T197","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T198","pred":"source","obj":"LitCovid-sample-CHEBI"}]},{"project":"LitCovid-sample-sentences","denotations":[{"id":"T161","span":{"begin":0,"end":212},"obj":"Sentence"},{"id":"T162","span":{"begin":213,"end":259},"obj":"Sentence"},{"id":"T163","span":{"begin":260,"end":308},"obj":"Sentence"},{"id":"T164","span":{"begin":309,"end":349},"obj":"Sentence"},{"id":"T165","span":{"begin":350,"end":387},"obj":"Sentence"},{"id":"T166","span":{"begin":388,"end":425},"obj":"Sentence"},{"id":"T167","span":{"begin":426,"end":528},"obj":"Sentence"},{"id":"T168","span":{"begin":529,"end":709},"obj":"Sentence"},{"id":"T169","span":{"begin":710,"end":767},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T161","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T162","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T163","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T164","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T165","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T166","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T167","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T168","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T169","pred":"source","obj":"LitCovid-sample-sentences"}]},{"project":"LitCovid-sample-Pubtator","denotations":[{"id":"911","span":{"begin":63,"end":73},"obj":"Chemical"},{"id":"912","span":{"begin":547,"end":559},"obj":"Chemical"},{"id":"913","span":{"begin":565,"end":572},"obj":"Chemical"},{"id":"914","span":{"begin":625,"end":632},"obj":"Chemical"},{"id":"915","span":{"begin":667,"end":671},"obj":"Chemical"}],"attributes":[{"id":"A913","pred":"pubann:denotes","subj":"913","obj":"MESH:D011134"},{"id":"A914","pred":"pubann:denotes","subj":"914","obj":"MESH:D011134"},{"id":"A915","pred":"pubann:denotes","subj":"915","obj":"MESH:C024865"},{"subj":"911","pred":"source","obj":"LitCovid-sample-Pubtator"},{"subj":"912","pred":"source","obj":"LitCovid-sample-Pubtator"},{"subj":"913","pred":"source","obj":"LitCovid-sample-Pubtator"},{"subj":"914","pred":"source","obj":"LitCovid-sample-Pubtator"},{"subj":"915","pred":"source","obj":"LitCovid-sample-Pubtator"}]},{"project":"LitCovid-sample-PD-GO-BP-0","denotations":[{"id":"T58","span":{"begin":589,"end":598},"obj":"http://purl.obolibrary.org/obo/GO_0007586"}],"attributes":[{"subj":"T58","pred":"source","obj":"LitCovid-sample-PD-GO-BP-0"}]},{"project":"LitCovid-sample-GO-BP","denotations":[{"id":"T51","span":{"begin":589,"end":598},"obj":"http://purl.obolibrary.org/obo/GO_0007586"}],"attributes":[{"subj":"T51","pred":"source","obj":"LitCovid-sample-GO-BP"}]},{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T56","span":{"begin":589,"end":598},"obj":"http://purl.obolibrary.org/obo/GO_0007586"}],"attributes":[{"subj":"T56","pred":"source","obj":"LitCovid-PD-GO-BP"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T161","span":{"begin":0,"end":212},"obj":"Sentence"},{"id":"T162","span":{"begin":213,"end":259},"obj":"Sentence"},{"id":"T163","span":{"begin":260,"end":308},"obj":"Sentence"},{"id":"T164","span":{"begin":309,"end":349},"obj":"Sentence"},{"id":"T165","span":{"begin":350,"end":387},"obj":"Sentence"},{"id":"T166","span":{"begin":388,"end":425},"obj":"Sentence"},{"id":"T167","span":{"begin":426,"end":528},"obj":"Sentence"},{"id":"T168","span":{"begin":529,"end":709},"obj":"Sentence"},{"id":"T169","span":{"begin":710,"end":767},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T161","pred":"source","obj":"LitCovid-sentences"},{"subj":"T162","pred":"source","obj":"LitCovid-sentences"},{"subj":"T163","pred":"source","obj":"LitCovid-sentences"},{"subj":"T164","pred":"source","obj":"LitCovid-sentences"},{"subj":"T165","pred":"source","obj":"LitCovid-sentences"},{"subj":"T166","pred":"source","obj":"LitCovid-sentences"},{"subj":"T167","pred":"source","obj":"LitCovid-sentences"},{"subj":"T168","pred":"source","obj":"LitCovid-sentences"},{"subj":"T169","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#e5ec93","default":true},{"id":"LitCovid-PD-FMA-UBERON","color":"#d993ec"},{"id":"LitCovid-PD-CLO","color":"#93ecbf"},{"id":"LitCovid-PD-CHEBI","color":"#eca593"},{"id":"LitCovid-sample-PD-FMA","color":"#939bec"},{"id":"LitCovid-sample-CHEBI","color":"#b5ec93"},{"id":"LitCovid-sample-sentences","color":"#ec93cf"},{"id":"LitCovid-sample-Pubtator","color":"#93e9ec"},{"id":"LitCovid-sample-PD-GO-BP-0","color":"#ecd593"},{"id":"LitCovid-sample-GO-BP","color":"#bb93ec"},{"id":"LitCovid-PD-GO-BP","color":"#93eca1"},{"id":"LitCovid-sentences","color":"#ec939f"}]}]}}