PMC:7253482 / 27895-28311 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"892","span":{"begin":57,"end":73},"obj":"Chemical"},{"id":"893","span":{"begin":112,"end":124},"obj":"Chemical"},{"id":"894","span":{"begin":175,"end":187},"obj":"Chemical"},{"id":"895","span":{"begin":201,"end":224},"obj":"Chemical"},{"id":"896","span":{"begin":232,"end":236},"obj":"Chemical"},{"id":"897","span":{"begin":253,"end":264},"obj":"Chemical"},{"id":"898","span":{"begin":299,"end":311},"obj":"Chemical"},{"id":"899","span":{"begin":321,"end":328},"obj":"Chemical"},{"id":"900","span":{"begin":349,"end":355},"obj":"Chemical"},{"id":"901","span":{"begin":356,"end":361},"obj":"Chemical"},{"id":"903","span":{"begin":396,"end":402},"obj":"Chemical"}],"attributes":[{"id":"A893","pred":"tao:has_database_id","subj":"893","obj":"MESH:D011342"},{"id":"A894","pred":"tao:has_database_id","subj":"894","obj":"MESH:D011342"},{"id":"A896","pred":"tao:has_database_id","subj":"896","obj":"MESH:D004121"},{"id":"A897","pred":"tao:has_database_id","subj":"897","obj":"MESH:D019342"},{"id":"A898","pred":"tao:has_database_id","subj":"898","obj":"MESH:D011342"},{"id":"A899","pred":"tao:has_database_id","subj":"899","obj":"MESH:D011134"},{"id":"A901","pred":"tao:has_database_id","subj":"901","obj":"MESH:D000577"},{"id":"A903","pred":"tao:has_database_id","subj":"903","obj":"MESH:D011134"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"d with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations).\n\nGlycan analysis by H"}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T186","span":{"begin":98,"end":106},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"},{"id":"T187","span":{"begin":146,"end":155},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"},{"id":"T188","span":{"begin":312,"end":320},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"}],"text":"d with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations).\n\nGlycan analysis by H"}

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{"project":"LitCovid-sample-CHEBI","denotations":[{"id":"T181","span":{"begin":66,"end":73},"obj":"Chemical"},{"id":"T182","span":{"begin":112,"end":124},"obj":"Chemical"},{"id":"T183","span":{"begin":156,"end":163},"obj":"Chemical"},{"id":"T184","span":{"begin":175,"end":187},"obj":"Chemical"},{"id":"T185","span":{"begin":201,"end":207},"obj":"Chemical"},{"id":"T186","span":{"begin":232,"end":236},"obj":"Chemical"},{"id":"T187","span":{"begin":253,"end":264},"obj":"Chemical"},{"id":"T188","span":{"begin":299,"end":311},"obj":"Chemical"},{"id":"T189","span":{"begin":321,"end":328},"obj":"Chemical"},{"id":"T190","span":{"begin":356,"end":361},"obj":"Chemical"},{"id":"T191","span":{"begin":396,"end":402},"obj":"Chemical"}],"attributes":[{"id":"A191","pred":"chebi_id","subj":"T191","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A189","pred":"chebi_id","subj":"T189","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A188","pred":"chebi_id","subj":"T188","obj":"http://purl.obolibrary.org/obo/CHEBI_8428"},{"id":"A187","pred":"chebi_id","subj":"T187","obj":"http://purl.obolibrary.org/obo/CHEBI_15366"},{"id":"A190","pred":"chebi_id","subj":"T190","obj":"http://purl.obolibrary.org/obo/CHEBI_32988"},{"id":"A185","pred":"chebi_id","subj":"T185","obj":"http://purl.obolibrary.org/obo/CHEBI_26708"},{"id":"A184","pred":"chebi_id","subj":"T184","obj":"http://purl.obolibrary.org/obo/CHEBI_8428"},{"id":"A186","pred":"chebi_id","subj":"T186","obj":"http://purl.obolibrary.org/obo/CHEBI_28262"},{"id":"A182","pred":"chebi_id","subj":"T182","obj":"http://purl.obolibrary.org/obo/CHEBI_8428"},{"id":"A183","pred":"chebi_id","subj":"T183","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"},{"id":"A181","pred":"chebi_id","subj":"T181","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"}],"text":"d with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations).\n\nGlycan analysis by H"}

{"project":"LitCovid-sample-sentences","denotations":[{"id":"T158","span":{"begin":36,"end":298},"obj":"Sentence"},{"id":"T159","span":{"begin":299,"end":394},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"d with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations).\n\nGlycan analysis by H"}

{"project":"LitCovid-sample-Pubtator","denotations":[{"id":"892","span":{"begin":57,"end":73},"obj":"Chemical"},{"id":"893","span":{"begin":112,"end":124},"obj":"Chemical"},{"id":"894","span":{"begin":175,"end":187},"obj":"Chemical"},{"id":"895","span":{"begin":201,"end":224},"obj":"Chemical"},{"id":"896","span":{"begin":232,"end":236},"obj":"Chemical"},{"id":"897","span":{"begin":253,"end":264},"obj":"Chemical"},{"id":"898","span":{"begin":299,"end":311},"obj":"Chemical"},{"id":"910","span":{"begin":321,"end":328},"obj":"Chemical"},{"id":"899","span":{"begin":321,"end":328},"obj":"Chemical"},{"id":"900","span":{"begin":349,"end":355},"obj":"Chemical"},{"id":"901","span":{"begin":356,"end":361},"obj":"Chemical"},{"id":"903","span":{"begin":396,"end":402},"obj":"Chemical"}],"attributes":[{"id":"A897","pred":"pubann:denotes","subj":"897","obj":"MESH:D019342"},{"id":"A894","pred":"pubann:denotes","subj":"894","obj":"MESH:D011342"},{"id":"A899","pred":"pubann:denotes","subj":"899","obj":"MESH:D011134"},{"id":"A910","pred":"pubann:denotes","subj":"910","obj":"MESH:D011134"},{"id":"A903","pred":"pubann:denotes","subj":"903","obj":"MESH:D011134"},{"id":"A898","pred":"pubann:denotes","subj":"898","obj":"MESH:D011342"},{"id":"A896","pred":"pubann:denotes","subj":"896","obj":"MESH:D004121"},{"id":"A901","pred":"pubann:denotes","subj":"901","obj":"MESH:D000577"},{"id":"A893","pred":"pubann:denotes","subj":"893","obj":"MESH:D011342"}],"text":"d with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations).\n\nGlycan analysis by H"}

{"project":"LitCovid-sample-UniProt","denotations":[{"id":"T5818","span":{"begin":7,"end":13},"obj":"Protein"}],"attributes":[{"id":"A5818","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q9TW67"},{"id":"A5819","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q9NR70"},{"id":"A5820","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q9NBD6"},{"id":"A5821","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q9JI78"},{"id":"A5822","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q9CTK3"},{"id":"A5823","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q9BVR8"},{"id":"A5824","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q96IV0"},{"id":"A5825","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q8K113"},{"id":"A5826","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q6PJD8"},{"id":"A5827","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q5ZJM3"},{"id":"A5828","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q5XI55"},{"id":"A5829","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q5WNE3"},{"id":"A5830","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q59FB1"},{"id":"A5831","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q503I8"},{"id":"A5832","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/Q4R6F3"},{"id":"A5833","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/B4DJE9"},{"id":"A5834","pred":"uniprot_id","subj":"T5818","obj":"https://www.uniprot.org/uniprot/A8X5N2"}],"text":"d with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations).\n\nGlycan analysis by H"}

{"project":"LitCovid-sample-PD-GO-BP-0","denotations":[{"id":"T57","span":{"begin":7,"end":15},"obj":"http://purl.obolibrary.org/obo/GO_0000224"}],"text":"d with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations).\n\nGlycan analysis by H"}

{"project":"LitCovid-sentences","denotations":[{"id":"T158","span":{"begin":36,"end":298},"obj":"Sentence"},{"id":"T159","span":{"begin":299,"end":394},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"d with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations).\n\nGlycan analysis by H"}