PMC:7253482 / 27714-28289 JSONTXT

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{"project":"LitCovid-sample-sentences","denotations":[{"id":"T156","span":{"begin":0,"end":121},"obj":"Sentence"},{"id":"T157","span":{"begin":122,"end":216},"obj":"Sentence"},{"id":"T158","span":{"begin":217,"end":479},"obj":"Sentence"},{"id":"T159","span":{"begin":480,"end":575},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Excised coronavirus S gel bands were washed alternately with acetonitrile and water before drying in a vacuum centrifuge. The bands were rehydrated with 100 μL of water and incubated with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations)."}

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{"project":"LitCovid-sample-PD-GO-BP-0","denotations":[{"id":"T57","span":{"begin":188,"end":196},"obj":"http://purl.obolibrary.org/obo/GO_0000224"}],"text":"Excised coronavirus S gel bands were washed alternately with acetonitrile and water before drying in a vacuum centrifuge. The bands were rehydrated with 100 μL of water and incubated with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T156","span":{"begin":0,"end":121},"obj":"Sentence"},{"id":"T157","span":{"begin":122,"end":216},"obj":"Sentence"},{"id":"T158","span":{"begin":217,"end":479},"obj":"Sentence"},{"id":"T159","span":{"begin":480,"end":575},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Excised coronavirus S gel bands were washed alternately with acetonitrile and water before drying in a vacuum centrifuge. The bands were rehydrated with 100 μL of water and incubated with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations)."}