PMC:7252096 / 87134-87676 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T982","span":{"begin":77,"end":84},"obj":"Body_part"},{"id":"T983","span":{"begin":164,"end":170},"obj":"Body_part"},{"id":"T984","span":{"begin":321,"end":327},"obj":"Body_part"},{"id":"T985","span":{"begin":372,"end":377},"obj":"Body_part"},{"id":"T986","span":{"begin":482,"end":486},"obj":"Body_part"},{"id":"T987","span":{"begin":527,"end":529},"obj":"Body_part"}],"attributes":[{"id":"A982","pred":"fma_id","subj":"T982","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A983","pred":"fma_id","subj":"T983","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A984","pred":"fma_id","subj":"T984","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A985","pred":"fma_id","subj":"T985","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A986","pred":"fma_id","subj":"T986","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A987","pred":"fma_id","subj":"T987","obj":"http://purl.org/sig/ont/fma/fma13444"},{"id":"A988","pred":"fma_id","subj":"T987","obj":"http://purl.org/sig/ont/fma/fma68614"}],"text":"Animals were perfused with 0.5 L of PBS/kg immediately following euthanasia, tissues were isolated and placed in RPMI + 10% FBS and kept on ice until dissociation. Tissue sections were digested by mincing and incubating with collagenase IV (Life Technologies) and DNase I (Roche) at 37°C for 1 h with agitation. Digested tissue was passed through a 100 μm metal strainer, cells were pelleted by centrifugation at 300 g, rinsed with RPMI + 10% FBS, counted, and prepared as a single cell suspension for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T189","span":{"begin":164,"end":170},"obj":"Body_part"},{"id":"T190","span":{"begin":321,"end":327},"obj":"Body_part"}],"attributes":[{"id":"A189","pred":"uberon_id","subj":"T189","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A190","pred":"uberon_id","subj":"T190","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"text":"Animals were perfused with 0.5 L of PBS/kg immediately following euthanasia, tissues were isolated and placed in RPMI + 10% FBS and kept on ice until dissociation. Tissue sections were digested by mincing and incubating with collagenase IV (Life Technologies) and DNase I (Roche) at 37°C for 1 h with agitation. Digested tissue was passed through a 100 μm metal strainer, cells were pelleted by centrifugation at 300 g, rinsed with RPMI + 10% FBS, counted, and prepared as a single cell suspension for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1449","span":{"begin":0,"end":7},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_33208"},{"id":"T1450","span":{"begin":347,"end":348},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1451","span":{"begin":372,"end":377},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1452","span":{"begin":473,"end":474},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1453","span":{"begin":482,"end":486},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Animals were perfused with 0.5 L of PBS/kg immediately following euthanasia, tissues were isolated and placed in RPMI + 10% FBS and kept on ice until dissociation. Tissue sections were digested by mincing and incubating with collagenase IV (Life Technologies) and DNase I (Roche) at 37°C for 1 h with agitation. Digested tissue was passed through a 100 μm metal strainer, cells were pelleted by centrifugation at 300 g, rinsed with RPMI + 10% FBS, counted, and prepared as a single cell suspension for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T124","span":{"begin":237,"end":239},"obj":"Chemical"}],"attributes":[{"id":"A88241","pred":"chebi_id","subj":"T124","obj":"http://purl.obolibrary.org/obo/CHEBI_74327"}],"text":"Animals were perfused with 0.5 L of PBS/kg immediately following euthanasia, tissues were isolated and placed in RPMI + 10% FBS and kept on ice until dissociation. Tissue sections were digested by mincing and incubating with collagenase IV (Life Technologies) and DNase I (Roche) at 37°C for 1 h with agitation. Digested tissue was passed through a 100 μm metal strainer, cells were pelleted by centrifugation at 300 g, rinsed with RPMI + 10% FBS, counted, and prepared as a single cell suspension for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T56","span":{"begin":264,"end":271},"obj":"http://purl.obolibrary.org/obo/GO_0004530"}],"text":"Animals were perfused with 0.5 L of PBS/kg immediately following euthanasia, tissues were isolated and placed in RPMI + 10% FBS and kept on ice until dissociation. Tissue sections were digested by mincing and incubating with collagenase IV (Life Technologies) and DNase I (Roche) at 37°C for 1 h with agitation. Digested tissue was passed through a 100 μm metal strainer, cells were pelleted by centrifugation at 300 g, rinsed with RPMI + 10% FBS, counted, and prepared as a single cell suspension for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T620","span":{"begin":0,"end":163},"obj":"Sentence"},{"id":"T621","span":{"begin":164,"end":311},"obj":"Sentence"},{"id":"T622","span":{"begin":312,"end":542},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Animals were perfused with 0.5 L of PBS/kg immediately following euthanasia, tissues were isolated and placed in RPMI + 10% FBS and kept on ice until dissociation. Tissue sections were digested by mincing and incubating with collagenase IV (Life Technologies) and DNase I (Roche) at 37°C for 1 h with agitation. Digested tissue was passed through a 100 μm metal strainer, cells were pelleted by centrifugation at 300 g, rinsed with RPMI + 10% FBS, counted, and prepared as a single cell suspension for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-HP","denotations":[{"id":"T20","span":{"begin":301,"end":310},"obj":"Phenotype"}],"attributes":[{"id":"A20","pred":"hp_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/HP_0000713"}],"text":"Animals were perfused with 0.5 L of PBS/kg immediately following euthanasia, tissues were isolated and placed in RPMI + 10% FBS and kept on ice until dissociation. Tissue sections were digested by mincing and incubating with collagenase IV (Life Technologies) and DNase I (Roche) at 37°C for 1 h with agitation. Digested tissue was passed through a 100 μm metal strainer, cells were pelleted by centrifugation at 300 g, rinsed with RPMI + 10% FBS, counted, and prepared as a single cell suspension for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"2430","span":{"begin":36,"end":39},"obj":"Chemical"},{"id":"2431","span":{"begin":113,"end":117},"obj":"Chemical"},{"id":"2432","span":{"begin":432,"end":436},"obj":"Chemical"},{"id":"2433","span":{"begin":301,"end":310},"obj":"Disease"}],"attributes":[{"id":"A2430","pred":"tao:has_database_id","subj":"2430","obj":"MESH:D007854"},{"id":"A2433","pred":"tao:has_database_id","subj":"2433","obj":"MESH:D011595"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Animals were perfused with 0.5 L of PBS/kg immediately following euthanasia, tissues were isolated and placed in RPMI + 10% FBS and kept on ice until dissociation. Tissue sections were digested by mincing and incubating with collagenase IV (Life Technologies) and DNase I (Roche) at 37°C for 1 h with agitation. Digested tissue was passed through a 100 μm metal strainer, cells were pelleted by centrifugation at 300 g, rinsed with RPMI + 10% FBS, counted, and prepared as a single cell suspension for scRNA-seq using Seq-Well v1 (see below)."}