PMC:7199903 / 5315-6389 JSON TXT

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LitCovid-PD-FMA-UBERON

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T31","span":{"begin":54,"end":66},"obj":"Body_part"},{"id":"T32","span":{"begin":117,"end":129},"obj":"Body_part"},{"id":"T33","span":{"begin":274,"end":281},"obj":"Body_part"},{"id":"T34","span":{"begin":415,"end":420},"obj":"Body_part"},{"id":"T35","span":{"begin":553,"end":560},"obj":"Body_part"},{"id":"T36","span":{"begin":619,"end":625},"obj":"Body_part"},{"id":"T37","span":{"begin":637,"end":642},"obj":"Body_part"},{"id":"T38","span":{"begin":690,"end":695},"obj":"Body_part"},{"id":"T39","span":{"begin":879,"end":886},"obj":"Body_part"},{"id":"T40","span":{"begin":926,"end":933},"obj":"Body_part"},{"id":"T41","span":{"begin":965,"end":972},"obj":"Body_part"}],"attributes":[{"id":"A31","pred":"fma_id","subj":"T31","obj":"http://purl.org/sig/ont/fma/fma62925"},{"id":"A32","pred":"fma_id","subj":"T32","obj":"http://purl.org/sig/ont/fma/fma62925"},{"id":"A33","pred":"fma_id","subj":"T33","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A34","pred":"fma_id","subj":"T34","obj":"http://purl.org/sig/ont/fma/fma60992"},{"id":"A35","pred":"fma_id","subj":"T35","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A36","pred":"fma_id","subj":"T36","obj":"http://purl.org/sig/ont/fma/fma7203"},{"id":"A37","pred":"fma_id","subj":"T37","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A38","pred":"fma_id","subj":"T38","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A39","pred":"fma_id","subj":"T39","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A40","pred":"fma_id","subj":"T40","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A41","pred":"fma_id","subj":"T41","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-PD-UBERON

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T2","span":{"begin":415,"end":420},"obj":"Body_part"},{"id":"T3","span":{"begin":619,"end":625},"obj":"Body_part"}],"attributes":[{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0002113"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-PD-MONDO

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T25","span":{"begin":41,"end":49},"obj":"Disease"},{"id":"T26","span":{"begin":104,"end":112},"obj":"Disease"},{"id":"T27","span":{"begin":326,"end":329},"obj":"Disease"},{"id":"T29","span":{"begin":540,"end":548},"obj":"Disease"},{"id":"T30","span":{"begin":866,"end":874},"obj":"Disease"},{"id":"T31","span":{"begin":913,"end":921},"obj":"Disease"}],"attributes":[{"id":"A25","pred":"mondo_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A26","pred":"mondo_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A27","pred":"mondo_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A28","pred":"mondo_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"id":"A29","pred":"mondo_id","subj":"T29","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A30","pred":"mondo_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A31","pred":"mondo_id","subj":"T31","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-PD-CLO

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T38","span":{"begin":69,"end":70},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T39","span":{"begin":184,"end":187},"obj":"http://purl.obolibrary.org/obo/CLO_0009141"},{"id":"T40","span":{"begin":184,"end":187},"obj":"http://purl.obolibrary.org/obo/CLO_0050980"},{"id":"T41","span":{"begin":370,"end":377},"obj":"http://purl.obolibrary.org/obo/PR_000018263"},{"id":"T42","span":{"begin":482,"end":483},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T43","span":{"begin":603,"end":608},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T44","span":{"begin":609,"end":625},"obj":"http://www.ebi.ac.uk/efo/EFO_0000927"},{"id":"T45","span":{"begin":632,"end":642},"obj":"http://purl.obolibrary.org/obo/CLO_0054159"},{"id":"T46","span":{"begin":690,"end":695},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-PD-CHEBI

LitCovid-sample-PD-IDO

{"project":"LitCovid-sample-PD-IDO","denotations":[{"id":"T30","span":{"begin":637,"end":642},"obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"id":"T31","span":{"begin":690,"end":695},"obj":"http://purl.obolibrary.org/obo/CL_0000000"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-Enju

LitCovid-sample-PD-FMA

{"project":"LitCovid-sample-PD-FMA","denotations":[{"id":"T31","span":{"begin":54,"end":66},"obj":"Body_part"},{"id":"T32","span":{"begin":117,"end":129},"obj":"Body_part"},{"id":"T33","span":{"begin":274,"end":281},"obj":"Body_part"},{"id":"T34","span":{"begin":415,"end":420},"obj":"Body_part"},{"id":"T35","span":{"begin":553,"end":560},"obj":"Body_part"},{"id":"T36","span":{"begin":619,"end":625},"obj":"Body_part"},{"id":"T37","span":{"begin":637,"end":642},"obj":"Body_part"},{"id":"T38","span":{"begin":690,"end":695},"obj":"Body_part"},{"id":"T39","span":{"begin":879,"end":886},"obj":"Body_part"},{"id":"T40","span":{"begin":926,"end":933},"obj":"Body_part"},{"id":"T41","span":{"begin":965,"end":972},"obj":"Body_part"}],"attributes":[{"id":"A33","pred":"fma_id","subj":"T33","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A31","pred":"fma_id","subj":"T31","obj":"http://purl.org/sig/ont/fma/fma62925"},{"id":"A35","pred":"fma_id","subj":"T35","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A38","pred":"fma_id","subj":"T38","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A40","pred":"fma_id","subj":"T40","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A41","pred":"fma_id","subj":"T41","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A39","pred":"fma_id","subj":"T39","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A37","pred":"fma_id","subj":"T37","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A34","pred":"fma_id","subj":"T34","obj":"http://purl.org/sig/ont/fma/fma60992"},{"id":"A36","pred":"fma_id","subj":"T36","obj":"http://purl.org/sig/ont/fma/fma7203"},{"id":"A32","pred":"fma_id","subj":"T32","obj":"http://purl.org/sig/ont/fma/fma62925"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-CHEBI

{"project":"LitCovid-sample-CHEBI","denotations":[{"id":"T31","span":{"begin":54,"end":66},"obj":"Chemical"},{"id":"T32","span":{"begin":117,"end":129},"obj":"Chemical"},{"id":"T33","span":{"begin":228,"end":231},"obj":"Chemical"},{"id":"T36","span":{"begin":252,"end":255},"obj":"Chemical"},{"id":"T38","span":{"begin":260,"end":263},"obj":"Chemical"},{"id":"T40","span":{"begin":268,"end":271},"obj":"Chemical"},{"id":"T41","span":{"begin":274,"end":281},"obj":"Chemical"},{"id":"T42","span":{"begin":370,"end":377},"obj":"Chemical"},{"id":"T43","span":{"begin":485,"end":488},"obj":"Chemical"},{"id":"T44","span":{"begin":489,"end":503},"obj":"Chemical"},{"id":"T47","span":{"begin":553,"end":560},"obj":"Chemical"},{"id":"T48","span":{"begin":879,"end":886},"obj":"Chemical"},{"id":"T49","span":{"begin":926,"end":933},"obj":"Chemical"},{"id":"T50","span":{"begin":965,"end":972},"obj":"Chemical"}],"attributes":[{"id":"A31","pred":"chebi_id","subj":"T31","obj":"http://purl.obolibrary.org/obo/CHEBI_17089"},{"id":"A33","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_17196"},{"id":"A34","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_22653"},{"id":"A35","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_50347"},{"id":"A44","pred":"chebi_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/CHEBI_51135"},{"id":"A45","pred":"chebi_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/CHEBI_53656"},{"id":"A46","pred":"chebi_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/CHEBI_60766"},{"id":"A50","pred":"chebi_id","subj":"T50","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A36","pred":"chebi_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/CHEBI_17115"},{"id":"A37","pred":"chebi_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/CHEBI_29999"},{"id":"A32","pred":"chebi_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/CHEBI_17089"},{"id":"A40","pred":"chebi_id","subj":"T40","obj":"http://purl.obolibrary.org/obo/CHEBI_50342"},{"id":"A49","pred":"chebi_id","subj":"T49","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A41","pred":"chebi_id","subj":"T41","obj":"http://purl.obolibrary.org/obo/CHEBI_16541"},{"id":"A43","pred":"chebi_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"},{"id":"A47","pred":"chebi_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A42","pred":"chebi_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/CHEBI_16670"},{"id":"A38","pred":"chebi_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/CHEBI_16857"},{"id":"A39","pred":"chebi_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/CHEBI_30013"},{"id":"A48","pred":"chebi_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-PD-NCBITaxon

{"project":"LitCovid-sample-PD-NCBITaxon","denotations":[{"id":"T23","span":{"begin":41,"end":51},"obj":"Species"},{"id":"T24","span":{"begin":104,"end":114},"obj":"Species"},{"id":"T25","span":{"begin":540,"end":550},"obj":"Species"},{"id":"T26","span":{"begin":603,"end":608},"obj":"Species"},{"id":"T27","span":{"begin":866,"end":876},"obj":"Species"},{"id":"T28","span":{"begin":913,"end":923},"obj":"Species"}],"attributes":[{"id":"A23","pred":"ncbi_taxonomy_id","subj":"T23","obj":"NCBItxid:2697049"},{"id":"A24","pred":"ncbi_taxonomy_id","subj":"T24","obj":"NCBItxid:2697049"},{"id":"A25","pred":"ncbi_taxonomy_id","subj":"T25","obj":"NCBItxid:2697049"},{"id":"A26","pred":"ncbi_taxonomy_id","subj":"T26","obj":"NCBItxid:9606"},{"id":"A27","pred":"ncbi_taxonomy_id","subj":"T27","obj":"NCBItxid:2697049"},{"id":"A28","pred":"ncbi_taxonomy_id","subj":"T28","obj":"NCBItxid:2697049"}],"namespaces":[{"prefix":"NCBItxid","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-sentences

{"project":"LitCovid-sample-sentences","denotations":[{"id":"T39","span":{"begin":0,"end":67},"obj":"Sentence"},{"id":"T40","span":{"begin":68,"end":130},"obj":"Sentence"},{"id":"T41","span":{"begin":131,"end":273},"obj":"Sentence"},{"id":"T42","span":{"begin":274,"end":643},"obj":"Sentence"},{"id":"T43","span":{"begin":644,"end":887},"obj":"Sentence"},{"id":"T44","span":{"begin":888,"end":1074},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-PD-UBERON

{"project":"LitCovid-sample-PD-UBERON","denotations":[{"id":"T2","span":{"begin":415,"end":420},"obj":"Body_part"},{"id":"T3","span":{"begin":619,"end":625},"obj":"Body_part"}],"attributes":[{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0002113"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-PD-MONDO

{"project":"LitCovid-sample-PD-MONDO","denotations":[{"id":"T23","span":{"begin":41,"end":51},"obj":"Disease"},{"id":"T24","span":{"begin":104,"end":114},"obj":"Disease"},{"id":"T25","span":{"begin":540,"end":550},"obj":"Disease"},{"id":"T26","span":{"begin":866,"end":876},"obj":"Disease"},{"id":"T27","span":{"begin":913,"end":923},"obj":"Disease"}],"attributes":[{"id":"A23","pred":"mondo_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A24","pred":"mondo_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A25","pred":"mondo_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A26","pred":"mondo_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A27","pred":"mondo_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-Pubtator

{"project":"LitCovid-sample-Pubtator","denotations":[{"id":"151","span":{"begin":41,"end":51},"obj":"Species"},{"id":"171","span":{"begin":924,"end":933},"obj":"Gene"},{"id":"172","span":{"begin":877,"end":886},"obj":"Gene"},{"id":"173","span":{"begin":551,"end":560},"obj":"Gene"},{"id":"174","span":{"begin":249,"end":250},"obj":"Gene"},{"id":"175","span":{"begin":184,"end":185},"obj":"Gene"},{"id":"176","span":{"begin":115,"end":116},"obj":"Gene"},{"id":"177","span":{"begin":104,"end":114},"obj":"Species"},{"id":"178","span":{"begin":540,"end":550},"obj":"Species"},{"id":"179","span":{"begin":603,"end":608},"obj":"Species"},{"id":"180","span":{"begin":866,"end":876},"obj":"Species"},{"id":"181","span":{"begin":913,"end":923},"obj":"Species"},{"id":"182","span":{"begin":148,"end":149},"obj":"Chemical"},{"id":"183","span":{"begin":228,"end":231},"obj":"Chemical"},{"id":"184","span":{"begin":252,"end":255},"obj":"Chemical"},{"id":"185","span":{"begin":260,"end":263},"obj":"Chemical"},{"id":"186","span":{"begin":268,"end":271},"obj":"Chemical"},{"id":"187","span":{"begin":485,"end":503},"obj":"Chemical"},{"id":"188","span":{"begin":609,"end":625},"obj":"Disease"},{"id":"189","span":{"begin":627,"end":636},"obj":"CellLine"}],"attributes":[{"id":"A172","pred":"pubann:denotes","subj":"172","obj":"Gene:43740568"},{"id":"A177","pred":"pubann:denotes","subj":"177","obj":"Tax:2697049"},{"id":"A183","pred":"pubann:denotes","subj":"183","obj":"MESH:D001216"},{"id":"A179","pred":"pubann:denotes","subj":"179","obj":"Tax:9606"},{"id":"A185","pred":"pubann:denotes","subj":"185","obj":"MESH:D013912"},{"id":"A171","pred":"pubann:denotes","subj":"171","obj":"Gene:43740568"},{"id":"A174","pred":"pubann:denotes","subj":"174","obj":"Gene:43740568"},{"id":"A151","pred":"pubann:denotes","subj":"151","obj":"Tax:2697049"},{"id":"A184","pred":"pubann:denotes","subj":"184","obj":"MESH:D012694"},{"id":"A186","pred":"pubann:denotes","subj":"186","obj":"MESH:D011392"},{"id":"A175","pred":"pubann:denotes","subj":"175","obj":"Gene:43740568"},{"id":"A180","pred":"pubann:denotes","subj":"180","obj":"Tax:2697049"},{"id":"A189","pred":"pubann:denotes","subj":"189","obj":"CVCL:6642"},{"id":"A178","pred":"pubann:denotes","subj":"178","obj":"Tax:2697049"},{"id":"A173","pred":"pubann:denotes","subj":"173","obj":"Gene:43740568"},{"id":"A176","pred":"pubann:denotes","subj":"176","obj":"Gene:43740568"},{"id":"A182","pred":"pubann:denotes","subj":"182","obj":"MESH:D009584"},{"id":"A188","pred":"pubann:denotes","subj":"188","obj":"MESH:D007674"},{"id":"A181","pred":"pubann:denotes","subj":"181","obj":"Tax:2697049"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-UniProt

LitCovid-sample-PD-MAT

{"project":"LitCovid-sample-PD-MAT","denotations":[{"id":"T1","span":{"begin":619,"end":625},"obj":"http://purl.obolibrary.org/obo/MAT_0000119"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-PD-GO-BP-0

{"project":"LitCovid-sample-PD-GO-BP-0","denotations":[{"id":"T24","span":{"begin":157,"end":170},"obj":"http://purl.obolibrary.org/obo/GO_0070085"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-GO-BP

{"project":"LitCovid-sample-GO-BP","denotations":[{"id":"T22","span":{"begin":157,"end":170},"obj":"http://purl.obolibrary.org/obo/GO_0070085"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-PD-GO-BP

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T24","span":{"begin":148,"end":170},"obj":"http://purl.obolibrary.org/obo/GO_0006487"},{"id":"T25","span":{"begin":157,"end":170},"obj":"http://purl.obolibrary.org/obo/GO_0070085"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-sample-Glycan

LitCovid-sentences

{"project":"LitCovid-sentences","denotations":[{"id":"T39","span":{"begin":0,"end":67},"obj":"Sentence"},{"id":"T40","span":{"begin":68,"end":130},"obj":"Sentence"},{"id":"T41","span":{"begin":131,"end":273},"obj":"Sentence"},{"id":"T42","span":{"begin":274,"end":643},"obj":"Sentence"},{"id":"T43","span":{"begin":644,"end":887},"obj":"Sentence"},{"id":"T44","span":{"begin":888,"end":1074},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Fig. 1 Expression and validation of the SARS-CoV-2 S glycoprotein.\n(A) Schematic representation of the SARS-CoV-2 S glycoprotein. The positions of N-linked glycosylation sequons (N-X-S/T, where X ≠ P) are shown as branches (N, Asn; X, any residue; S, Ser; T, Thr; P, Pro). Protein domains are illustrated: N-terminal domain (NTD), receptor binding domain (RBD), fusion peptide (FP), heptad repeat 1 (HR1), central helix (CH), connector domain (CD), and transmembrane domain (TM). (B) SDS–polyacrylamide gel electrophoresis analysis of the SARS-CoV-2 S protein (indicated by the arrowhead) expressed in human embryonic kidney (HEK) 293F cells. Lane 1: filtered supernatant from transfected cells; lane 2: flow-through from StrepTactin resin; lane 3: wash from StrepTactin resin; lane 4: elution from StrepTactin resin. (C) Negative-stain EM 2D class averages of the SARS-CoV-2 S protein. 2D class averages of the SARS-CoV-2 S protein are shown, confirming that the protein adopts the trimeric prefusion conformation matching the material used to determine the structure (4)."}

LitCovid-PubTator

PMC:7199903 / 5315-6389 JSONTXT

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