PMC:7199903 / 4170-4588 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T26","span":{"begin":63,"end":70},"obj":"Body_part"},{"id":"T27","span":{"begin":127,"end":134},"obj":"Body_part"}],"attributes":[{"id":"A26","pred":"fma_id","subj":"T26","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A27","pred":"fma_id","subj":"T27","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T22","span":{"begin":50,"end":58},"obj":"Disease"}],"attributes":[{"id":"A22","pred":"mondo_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T33","span":{"begin":367,"end":368},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T26","span":{"begin":63,"end":70},"obj":"Chemical"},{"id":"T27","span":{"begin":127,"end":134},"obj":"Chemical"},{"id":"T28","span":{"begin":311,"end":319},"obj":"Chemical"},{"id":"T29","span":{"begin":337,"end":339},"obj":"Chemical"}],"attributes":[{"id":"A26","pred":"chebi_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A27","pred":"chebi_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A28","pred":"chebi_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/CHEBI_10545"},{"id":"A29","pred":"chebi_id","subj":"T29","obj":"http://purl.obolibrary.org/obo/CHEBI_73507"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-PD-IDO","denotations":[{"id":"T27","span":{"begin":15,"end":19},"obj":"http://purl.obolibrary.org/obo/BFO_0000029"},{"id":"T28","span":{"begin":187,"end":197},"obj":"http://purl.obolibrary.org/obo/IDO_0000608"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

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resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-PD-FMA","denotations":[{"id":"T26","span":{"begin":63,"end":70},"obj":"Body_part"},{"id":"T27","span":{"begin":127,"end":134},"obj":"Body_part"}],"attributes":[{"id":"A26","pred":"fma_id","subj":"T26","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A27","pred":"fma_id","subj":"T27","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-CHEBI","denotations":[{"id":"T22","span":{"begin":63,"end":70},"obj":"Chemical"},{"id":"T23","span":{"begin":127,"end":134},"obj":"Chemical"}],"attributes":[{"id":"A22","pred":"chebi_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A23","pred":"chebi_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-PD-NCBITaxon","denotations":[{"id":"T20","span":{"begin":50,"end":60},"obj":"Species"}],"attributes":[{"id":"A20","pred":"ncbi_taxonomy_id","subj":"T20","obj":"NCBItxid:2697049"}],"namespaces":[{"prefix":"NCBItxid","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-sentences","denotations":[{"id":"T33","span":{"begin":0,"end":418},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-PD-MONDO","denotations":[{"id":"T20","span":{"begin":50,"end":60},"obj":"Disease"}],"attributes":[{"id":"A20","pred":"mondo_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-Pubtator","denotations":[{"id":"140","span":{"begin":61,"end":70},"obj":"Gene"},{"id":"142","span":{"begin":50,"end":60},"obj":"Species"},{"id":"145","span":{"begin":402,"end":413},"obj":"Chemical"}],"attributes":[{"id":"A140","pred":"pubann:denotes","subj":"140","obj":"Gene:43740568"},{"id":"A142","pred":"pubann:denotes","subj":"142","obj":"Tax:2697049"},{"id":"A145","pred":"pubann:denotes","subj":"145","obj":"MESH:C065629"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-UniProt","denotations":[{"id":"T303","span":{"begin":61,"end":70},"obj":"Protein"}],"attributes":[{"id":"A303","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9UIP0"},{"id":"A304","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9UIN9"},{"id":"A305","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9UIN8"},{"id":"A306","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9UIN7"},{"id":"A307","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9UIN6"},{"id":"A308","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9UBH8"},{"id":"A309","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9NRH8"},{"id":"A310","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9NRH7"},{"id":"A311","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9NRH6"},{"id":"A312","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9NRH5"},{"id":"A313","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9NRH4"},{"id":"A314","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9NPG5"},{"id":"A315","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9NPE0"},{"id":"A316","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q9NP52"},{"id":"A317","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q95IF9"},{"id":"A318","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q8N5P3"},{"id":"A319","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q8IZU6"},{"id":"A320","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q8IZU5"},{"id":"A321","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q8IZU4"},{"id":"A322","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q86Z04"},{"id":"A323","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q7YR44"},{"id":"A324","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q7LA71"},{"id":"A325","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q7LA70"},{"id":"A326","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q5STD2"},{"id":"A327","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q5SQ85"},{"id":"A328","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q1XI16"},{"id":"A329","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q1XI12"},{"id":"A330","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/Q15517"},{"id":"A331","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/O43509"},{"id":"A332","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/O19084"},{"id":"A333","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/B0UYZ7"},{"id":"A334","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/B0S7V2"},{"id":"A335","pred":"uniprot_id","subj":"T303","obj":"https://www.uniprot.org/uniprot/A5A6L9"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-PD-GO-BP-0","denotations":[{"id":"T23","span":{"begin":29,"end":42},"obj":"http://purl.obolibrary.org/obo/GO_0070085"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sample-GO-BP","denotations":[{"id":"T21","span":{"begin":29,"end":42},"obj":"http://purl.obolibrary.org/obo/GO_0070085"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T23","span":{"begin":29,"end":42},"obj":"http://purl.obolibrary.org/obo/GO_0070085"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T33","span":{"begin":0,"end":418},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"140","span":{"begin":61,"end":70},"obj":"Gene"},{"id":"142","span":{"begin":50,"end":60},"obj":"Species"},{"id":"145","span":{"begin":402,"end":413},"obj":"Chemical"}],"attributes":[{"id":"A140","pred":"tao:has_database_id","subj":"140","obj":"Gene:43740568"},{"id":"A142","pred":"tao:has_database_id","subj":"142","obj":"Tax:2697049"},{"id":"A145","pred":"tao:has_database_id","subj":"145","obj":"MESH:C065629"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4)."}