PMC:7152911 / 79409-80589 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"1481","span":{"begin":7,"end":12},"obj":"Chemical"},{"id":"1489","span":{"begin":497,"end":499},"obj":"Gene"},{"id":"1490","span":{"begin":450,"end":452},"obj":"Gene"},{"id":"1491","span":{"begin":343,"end":357},"obj":"Species"},{"id":"1492","span":{"begin":523,"end":528},"obj":"Species"},{"id":"1493","span":{"begin":551,"end":558},"obj":"Species"},{"id":"1494","span":{"begin":627,"end":641},"obj":"Species"},{"id":"1495","span":{"begin":381,"end":392},"obj":"Chemical"}],"attributes":[{"id":"A1489","pred":"tao:has_database_id","subj":"1489","obj":"Gene:21832"},{"id":"A1490","pred":"tao:has_database_id","subj":"1490","obj":"Gene:21832"},{"id":"A1491","pred":"tao:has_database_id","subj":"1491","obj":"Tax:90371"},{"id":"A1492","pred":"tao:has_database_id","subj":"1492","obj":"Tax:883761"},{"id":"A1493","pred":"tao:has_database_id","subj":"1493","obj":"Tax:9031"},{"id":"A1494","pred":"tao:has_database_id","subj":"1494","obj":"Tax:90371"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"3.1.3 Broth enrichment\nBroth enrichment is a technique used to increase the concentration of target species in the sample through growth or replication of target species prior to measurement, thereby increasing the number present for detection. The technique is commonly used in food safety applications. For example, Liebana et al. enriched S. typhimurium-spiked milk samples in Luria broth (LB) for 8 h to improve the assay LOD from 7.5 × 103 CFU/mL for the 50-min enriched sample to 0.108 CFU/mL (Liebana et al. 2009). Salam et al. enriched fresh chicken samples in enrichment buffer peptone for 18–24 h to recover injured S. typhimurium cells for detection via chronoamperometry (Salam and Tothill, 2009). While enrichment can be a useful sample preparation step when the target concentration is below the biosensor's LOD, it is inherently limited to viable and cultural organisms. Further, analysis of the results obtained from multiple samples should consider potential differences in the growth rate of bacteria across different samples. It is important to note that the need for sample enrichment significantly increases the TTR and impedes rapid and real-time detection."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T173","span":{"begin":365,"end":369},"obj":"Body_part"},{"id":"T174","span":{"begin":642,"end":647},"obj":"Body_part"}],"attributes":[{"id":"A173","pred":"fma_id","subj":"T173","obj":"http://purl.org/sig/ont/fma/fma62100"},{"id":"A174","pred":"fma_id","subj":"T174","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"3.1.3 Broth enrichment\nBroth enrichment is a technique used to increase the concentration of target species in the sample through growth or replication of target species prior to measurement, thereby increasing the number present for detection. The technique is commonly used in food safety applications. For example, Liebana et al. enriched S. typhimurium-spiked milk samples in Luria broth (LB) for 8 h to improve the assay LOD from 7.5 × 103 CFU/mL for the 50-min enriched sample to 0.108 CFU/mL (Liebana et al. 2009). Salam et al. enriched fresh chicken samples in enrichment buffer peptone for 18–24 h to recover injured S. typhimurium cells for detection via chronoamperometry (Salam and Tothill, 2009). While enrichment can be a useful sample preparation step when the target concentration is below the biosensor's LOD, it is inherently limited to viable and cultural organisms. Further, analysis of the results obtained from multiple samples should consider potential differences in the growth rate of bacteria across different samples. It is important to note that the need for sample enrichment significantly increases the TTR and impedes rapid and real-time detection."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T18","span":{"begin":365,"end":369},"obj":"Body_part"}],"attributes":[{"id":"A18","pred":"uberon_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/UBERON_0001913"}],"text":"3.1.3 Broth enrichment\nBroth enrichment is a technique used to increase the concentration of target species in the sample through growth or replication of target species prior to measurement, thereby increasing the number present for detection. The technique is commonly used in food safety applications. For example, Liebana et al. enriched S. typhimurium-spiked milk samples in Luria broth (LB) for 8 h to improve the assay LOD from 7.5 × 103 CFU/mL for the 50-min enriched sample to 0.108 CFU/mL (Liebana et al. 2009). Salam et al. enriched fresh chicken samples in enrichment buffer peptone for 18–24 h to recover injured S. typhimurium cells for detection via chronoamperometry (Salam and Tothill, 2009). While enrichment can be a useful sample preparation step when the target concentration is below the biosensor's LOD, it is inherently limited to viable and cultural organisms. Further, analysis of the results obtained from multiple samples should consider potential differences in the growth rate of bacteria across different samples. It is important to note that the need for sample enrichment significantly increases the TTR and impedes rapid and real-time detection."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T525","span":{"begin":44,"end":45},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T526","span":{"begin":551,"end":558},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T527","span":{"begin":600,"end":602},"obj":"http://purl.obolibrary.org/obo/CLO_0050510"},{"id":"T528","span":{"begin":642,"end":647},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T529","span":{"begin":735,"end":736},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T530","span":{"begin":876,"end":885},"obj":"http://purl.obolibrary.org/obo/OBI_0100026"},{"id":"T531","span":{"begin":876,"end":885},"obj":"http://purl.obolibrary.org/obo/UBERON_0000468"},{"id":"T532","span":{"begin":1011,"end":1019},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_2"}],"text":"3.1.3 Broth enrichment\nBroth enrichment is a technique used to increase the concentration of target species in the sample through growth or replication of target species prior to measurement, thereby increasing the number present for detection. The technique is commonly used in food safety applications. For example, Liebana et al. enriched S. typhimurium-spiked milk samples in Luria broth (LB) for 8 h to improve the assay LOD from 7.5 × 103 CFU/mL for the 50-min enriched sample to 0.108 CFU/mL (Liebana et al. 2009). Salam et al. enriched fresh chicken samples in enrichment buffer peptone for 18–24 h to recover injured S. typhimurium cells for detection via chronoamperometry (Salam and Tothill, 2009). While enrichment can be a useful sample preparation step when the target concentration is below the biosensor's LOD, it is inherently limited to viable and cultural organisms. Further, analysis of the results obtained from multiple samples should consider potential differences in the growth rate of bacteria across different samples. It is important to note that the need for sample enrichment significantly increases the TTR and impedes rapid and real-time detection."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T96613","span":{"begin":581,"end":587},"obj":"Chemical"}],"attributes":[{"id":"A30113","pred":"chebi_id","subj":"T96613","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"}],"text":"3.1.3 Broth enrichment\nBroth enrichment is a technique used to increase the concentration of target species in the sample through growth or replication of target species prior to measurement, thereby increasing the number present for detection. The technique is commonly used in food safety applications. For example, Liebana et al. enriched S. typhimurium-spiked milk samples in Luria broth (LB) for 8 h to improve the assay LOD from 7.5 × 103 CFU/mL for the 50-min enriched sample to 0.108 CFU/mL (Liebana et al. 2009). Salam et al. enriched fresh chicken samples in enrichment buffer peptone for 18–24 h to recover injured S. typhimurium cells for detection via chronoamperometry (Salam and Tothill, 2009). While enrichment can be a useful sample preparation step when the target concentration is below the biosensor's LOD, it is inherently limited to viable and cultural organisms. Further, analysis of the results obtained from multiple samples should consider potential differences in the growth rate of bacteria across different samples. It is important to note that the need for sample enrichment significantly increases the TTR and impedes rapid and real-time detection."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T33","span":{"begin":131,"end":137},"obj":"http://purl.obolibrary.org/obo/GO_0040007"},{"id":"T34","span":{"begin":996,"end":1002},"obj":"http://purl.obolibrary.org/obo/GO_0040007"}],"text":"3.1.3 Broth enrichment\nBroth enrichment is a technique used to increase the concentration of target species in the sample through growth or replication of target species prior to measurement, thereby increasing the number present for detection. The technique is commonly used in food safety applications. For example, Liebana et al. enriched S. typhimurium-spiked milk samples in Luria broth (LB) for 8 h to improve the assay LOD from 7.5 × 103 CFU/mL for the 50-min enriched sample to 0.108 CFU/mL (Liebana et al. 2009). Salam et al. enriched fresh chicken samples in enrichment buffer peptone for 18–24 h to recover injured S. typhimurium cells for detection via chronoamperometry (Salam and Tothill, 2009). While enrichment can be a useful sample preparation step when the target concentration is below the biosensor's LOD, it is inherently limited to viable and cultural organisms. Further, analysis of the results obtained from multiple samples should consider potential differences in the growth rate of bacteria across different samples. It is important to note that the need for sample enrichment significantly increases the TTR and impedes rapid and real-time detection."}

{"project":"LitCovid-sentences","denotations":[{"id":"T649","span":{"begin":0,"end":23},"obj":"Sentence"},{"id":"T650","span":{"begin":24,"end":245},"obj":"Sentence"},{"id":"T651","span":{"begin":246,"end":305},"obj":"Sentence"},{"id":"T652","span":{"begin":306,"end":515},"obj":"Sentence"},{"id":"T653","span":{"begin":516,"end":522},"obj":"Sentence"},{"id":"T654","span":{"begin":523,"end":710},"obj":"Sentence"},{"id":"T655","span":{"begin":711,"end":886},"obj":"Sentence"},{"id":"T656","span":{"begin":887,"end":1045},"obj":"Sentence"},{"id":"T657","span":{"begin":1046,"end":1180},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"3.1.3 Broth enrichment\nBroth enrichment is a technique used to increase the concentration of target species in the sample through growth or replication of target species prior to measurement, thereby increasing the number present for detection. The technique is commonly used in food safety applications. For example, Liebana et al. enriched S. typhimurium-spiked milk samples in Luria broth (LB) for 8 h to improve the assay LOD from 7.5 × 103 CFU/mL for the 50-min enriched sample to 0.108 CFU/mL (Liebana et al. 2009). Salam et al. enriched fresh chicken samples in enrichment buffer peptone for 18–24 h to recover injured S. typhimurium cells for detection via chronoamperometry (Salam and Tothill, 2009). While enrichment can be a useful sample preparation step when the target concentration is below the biosensor's LOD, it is inherently limited to viable and cultural organisms. Further, analysis of the results obtained from multiple samples should consider potential differences in the growth rate of bacteria across different samples. It is important to note that the need for sample enrichment significantly increases the TTR and impedes rapid and real-time detection."}

{"project":"2_test","denotations":[{"id":"32364936-19716286-7713105","span":{"begin":516,"end":520},"obj":"19716286"},{"id":"32364936-19233634-7713106","span":{"begin":704,"end":708},"obj":"19233634"}],"text":"3.1.3 Broth enrichment\nBroth enrichment is a technique used to increase the concentration of target species in the sample through growth or replication of target species prior to measurement, thereby increasing the number present for detection. The technique is commonly used in food safety applications. For example, Liebana et al. enriched S. typhimurium-spiked milk samples in Luria broth (LB) for 8 h to improve the assay LOD from 7.5 × 103 CFU/mL for the 50-min enriched sample to 0.108 CFU/mL (Liebana et al. 2009). Salam et al. enriched fresh chicken samples in enrichment buffer peptone for 18–24 h to recover injured S. typhimurium cells for detection via chronoamperometry (Salam and Tothill, 2009). While enrichment can be a useful sample preparation step when the target concentration is below the biosensor's LOD, it is inherently limited to viable and cultural organisms. Further, analysis of the results obtained from multiple samples should consider potential differences in the growth rate of bacteria across different samples. It is important to note that the need for sample enrichment significantly increases the TTR and impedes rapid and real-time detection."}