PMC:7152911 / 75288-76719 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7152911","sourcedb":"PMC","sourceid":"7152911","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7152911","text":"In addition to a physical device composed of an integrated transduction element and biorecognition element, an electrochemical biosensor-based assay for pathogen detection potentially involves processing steps associated with sample preparation and complementary physical systems for biosensor housing and sample handling. The associated protocols for sample preparation and sample handling are often referred to as the measurement format. Several important considerations regarding the measurement format for pathogen detection applications can be considered and vary based on the assay design, the biosensor performance (e.g., sensitivity and LOD), the volume, material properties, and composition of the pathogen-containing sample, and the application. For example, the use of DNA-based assays for pathogen detection typically requires sample preparation steps associated with the extraction of genetic material. Similarly, the use of a label-based biosensing approach requires sample preparation steps associated with target labeling. In cases where the concentration of target species in the sample is below the biosensor's LOD, pre-concentration steps may be required. Applications to process monitoring, such in bioreactor or tissue-chip monitoring, may require flow-based sample handling formats. We next discuss the measurement formats associated with pathogen detection in terms of sample preparation and sample handling.","tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T162","span":{"begin":780,"end":783},"obj":"Body_part"},{"id":"T163","span":{"begin":1233,"end":1239},"obj":"Body_part"}],"attributes":[{"id":"A162","pred":"fma_id","subj":"T162","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A163","pred":"fma_id","subj":"T163","obj":"http://purl.org/sig/ont/fma/fma9637"},{"subj":"T162","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T163","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T15","span":{"begin":1233,"end":1239},"obj":"Body_part"}],"attributes":[{"id":"A15","pred":"uberon_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"subj":"T15","pred":"source","obj":"LitCovid-PD-UBERON"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T503","span":{"begin":15,"end":16},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T504","span":{"begin":26,"end":32},"obj":"http://purl.obolibrary.org/obo/OBI_0000968"},{"id":"T505","span":{"begin":938,"end":939},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T506","span":{"begin":940,"end":945},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"},{"id":"T507","span":{"begin":1029,"end":1037},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"}],"attributes":[{"subj":"T503","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T504","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T505","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T506","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T507","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T29332","span":{"begin":743,"end":754},"obj":"Chemical"},{"id":"T38008","span":{"begin":780,"end":783},"obj":"Chemical"},{"id":"T62170","span":{"begin":940,"end":945},"obj":"Chemical"}],"attributes":[{"id":"A33860","pred":"chebi_id","subj":"T29332","obj":"http://purl.obolibrary.org/obo/CHEBI_33232"},{"id":"A36236","pred":"chebi_id","subj":"T38008","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A39926","pred":"chebi_id","subj":"T62170","obj":"http://purl.obolibrary.org/obo/CHEBI_35209"},{"subj":"T29332","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T38008","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T62170","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T31","span":{"begin":59,"end":71},"obj":"http://purl.obolibrary.org/obo/GO_0009293"}],"attributes":[{"subj":"T31","pred":"source","obj":"LitCovid-PD-GO-BP"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T616","span":{"begin":0,"end":322},"obj":"Sentence"},{"id":"T617","span":{"begin":323,"end":439},"obj":"Sentence"},{"id":"T618","span":{"begin":440,"end":755},"obj":"Sentence"},{"id":"T619","span":{"begin":756,"end":915},"obj":"Sentence"},{"id":"T620","span":{"begin":916,"end":1038},"obj":"Sentence"},{"id":"T621","span":{"begin":1039,"end":1174},"obj":"Sentence"},{"id":"T622","span":{"begin":1175,"end":1304},"obj":"Sentence"},{"id":"T623","span":{"begin":1305,"end":1431},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T616","pred":"source","obj":"LitCovid-sentences"},{"subj":"T617","pred":"source","obj":"LitCovid-sentences"},{"subj":"T618","pred":"source","obj":"LitCovid-sentences"},{"subj":"T619","pred":"source","obj":"LitCovid-sentences"},{"subj":"T620","pred":"source","obj":"LitCovid-sentences"},{"subj":"T621","pred":"source","obj":"LitCovid-sentences"},{"subj":"T622","pred":"source","obj":"LitCovid-sentences"},{"subj":"T623","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#93ec9f","default":true},{"id":"LitCovid-PD-UBERON","color":"#ec93a0"},{"id":"LitCovid-PD-CLO","color":"#93baec"},{"id":"LitCovid-PD-CHEBI","color":"#d4ec93"},{"id":"LitCovid-PD-GO-BP","color":"#e993ec"},{"id":"LitCovid-sentences","color":"#93eccf"}]}]}}