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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7152911","sourcedb":"PMC","sourceid":"7152911","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7152911","text":"Table 1 Classification of label-free electrochemical biosensors for detection of pathogens in terms of: target, working electrode, biorecognition element, electrochemical method, limit of detection, and electrochemical probe. Abbreviations: quartz crystal microbalance (QCM), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), plaque-forming unit (PFU), colony-forming unit (CFU), indium tin oxide (ITO), carbon nanotube (CNT), magnetic bead (MB), nanoparticle (NP), differential pulse voltammetry (DPV), square wave voltammetry (SWV), anodic stripping voltammetry (ASV), hemagglutination units (HAU), and median tissue culture infectious dose (TCID50).\nTarget Pathogen Working Electrode Biorecognition Element Electrochemical Method \u0026 Probe Limit of Detection Reference\nE. coli Au interdigitated microelectrode array polyclonal anti-E.coli EIS 104 CFU/mL Radke and Alocilja (2005)\nE. coli ITO electrode monoclonal anti-E. coli CV, EIS; Fe(CN)63-/4- 4 × 103 CFU/mL Zhang et al. (2005)\nE. coli chromium interdigitated microelectrode array anti-E. coli EIS – Suehiro et al. (2006)\nS. typhimurium ITO interdigitated microelectrode array anti-S. typhimurium EIS 10 CFU/mL Yang and Li (2006)\nV. cholerae carbon electrode polyclonal anti-V. cholerae amperometry 8 CFU/mL Sharma et al. (2006)\nE. coli Pt wire electrode polyclonal anti-E. coli potentiometry 9 × 105 CFU/mL Boehm et al. (2007)\nE. coli Au microelectrode polyclonal anti-E.coli EIS 10 CFU/mL Maalouf et al. (2007)\nL. monocytogenes TiO2 nanowires on Au electrode monoclonal anti-L. monocytogenes EIS 470 CFU/mL Wang et al. (2008)\nE. coli Au electrode polyclonal anti-E. coli CV, EIS; Fe(CN)63-/4- 50 CFU/mL Geng et al. (2008)\nS. typhimurium Au electrode polyclonal anti-S. typhimurium EIS; Fe(CN)63-/4- 10 CFU/mL Pournaras et al. (2008)\nS. typhimurium Au microelectrode anti-S. typhimurium EIS; Fe(CN)63-/4- 500 CFU/mL Nandakumar et al. (2008)\nE. coli graphite interdigitated microelectrode array E. coli-specific bacteriophages EIS 104 CFU/mL Shabani et al. (2008)\nS. typhimurium Au electrode polyclonal anti-S. typhimurium EIS 100 CFU/mL Mantzila et al. (2008)\nS. typhimurium macroporous silicon electrode anti-S. typhimurium EIS 103 CFU/mL Das et al. (2009)\nWest Nile virus (WNV) nanostructured alumina on Pt wire electrode monoclonal anti-WNV AC voltammetry 0.02 viruses/mL Nguyen et al. (2009)\nS. typhimurium Au electrode monoclonal anti-S. typhimurium EIS; Fe(CN)63-/4- 100 CFU/mL La Belle et al. (2009)\nS. typhimurium CNTs on carbon rod electrode anti-S. typhimurium aptamer potentiometry 0.2 CFU/mL Zelada-Guillen et al. (2009)\nE. coli Au electrode anti-E. coli CV, EIS; Fe(CN)63-/4- 3.3 CFU/mL Escamilla-Gomez et al. (2009)\nB. anthracis Ag electrode monoclonal and polyclonal anti-B. anthracis conductometry 420 spores/mL Pal and Alocilja (2009)\nE. coli polysilicon interdigitated microelectrode array polyclonal anti-E. coli EIS 300 CFU/mL de la Rica et al. (2009)\nE. coli Au interdigitated microelectrode array E. coli-specific bacteriophages EIS 104 CFU/mL Mejri et al. (2010)\nE. coli CNTs on carbon rod electrode anti-E. coli aptamer potentiometry 6 CFU/mL Zelada-Guillen et al. (2010)\nCampylobacter jejuni Fe3O4 nanoparticles on carbon electrode monoclonal anti-Flagellin A EIS; Fe(CN)63-/4- 103 CFU/mL Huang et al. (2010)\nmarine pathogenic sulphate-reducing bacteria (SRB) AuNPs on nickel foam electrode anti-SRB EIS 21 CFU/mL Wan et al. (2010)\nE. coli Ag nanofiber array electrode monoclonal and polyclonal anti-E. coli conductometry 61 CFU/mL Luo et al. (2010)\nbovine viral diarrhea virus (BVDV) Ag nanofiber array electrode monoclonal and polyclonal anti-BVDV conductometry 103 CCID/mL Luo et al. (2010)\nE. coli Au interdigitated microelectrode array magainin I peptide EIS 103 CFU/mL Mannoor et al. (2010)\nE. coli Au rod electrode concanavalin A lectin capacitive 12 CFU/mL Jantra et al. (2011)\nrotavirus graphene microelectrode monoclonal anti-rotavirus CV 103 PFU/mL Liu et al. (2011)\nhuman influenza A virus H3N2 Au electrode polyclonal anti-H3N2 EIS 8 ng/mL Hassen et al. (2011)\nE. coli Au microelectrode polyclonal anti-E. coli capacitive, EIS, CV; Fe(CN)63-/4- 220 CFU/mL Li et al. (2011)\nEnterobacter cloacae Au electrode concanavalin A lectin, ricinus communis agglutinin lectin CV, EIS; Fe(CN)63-/4- 1 × 103 CFU/mL Xi et al. (2011)\nE. coli Au electrode concanavalin A lectin, ricinus communis agglutinin lectin CV, EIS; Fe(CN)63-/4- 100 CFU/mL Xi et al. (2011)\nB. subtilis Au electrode concanavalin A lectin CV, EIS; Fe(CN)63-/4- 1 × 104 CFU/mL Xi et al. (2011)\nE. coli Pt wire electrode anti-E. coli EIS 100 CFU/mL Tan et al. (2011)\nS. aureus Pt wire electrode anti-S. aureus EIS 100 CFU/mL Tan et al. (2011)\nmarine pathogenic sulphate-reducing bacteria (SRB) graphene/chitosan composite on carbon electrode anti-SRB CV, EIS; Fe(CN)63-/4- 18 CFU/mL Wan et al. (2011)\nswine influenza virus (SIV) H1N1 PDDA/CNT composite on Au microelectrode anti-SIV conductometry 180 TCID50/mL Lee et al. (2011)\nE. coli graphene microelectrode anti-E. coli amperometry 10 CFU/mL Huang et al. (2011)\nE. coli PEDOT:PSS electrode anti-E. coli amperometry 103 CFU/mL He et al. (2012)\ndengue type 2 virus (DENV-2) nanostructured alumina on Pt wire electrode monoclonal anti-DENV-2 DPV;Ferrocene methanol 1 PFU/mL Cheng et al. (2012)\nDENV-2 nanostructured alumina on Pt wire electrode monoclonal anti-DENV-2 CV, EIS; Ferrocene methanol 1 PFU/mL Nguyen et al. (2012)\nhuman influenza A viruses H1N1 and H3N2 silicon nanowire electrode array anti-H1N1, anti-H3N2 conductometry 2.9 × 104 viruses/mL Shen et al. (2012)\nE. coli AuNP/Chitosan/CNT and SiO2/thionine NP composite on Au electrode monoclonal anti-E. coli CV 250 CFU/mL Li et al. (2012)\nE. coli CNT/polyallylamine composite on graphite electrode monoclonal anti-E. coli ASV 800 cells/mL Viswanathan et al. (2012)\nCampylobacter CNT/polyallylamine composite on graphite electrode monoclonal anti-Campylobacter ASV 400 cells/mL Viswanathan et al. (2012)\nS. typhimurium CNT/polyallylamine composite on graphite electrode monoclonal anti-S. typhimurium ASV 400 cells/mL Viswanathan et al. (2012)\nS. aureus CNT electrode anti-S. aureus aptamer potentiometry 800 CFU/mL Zelada-Guillen et al. (2012)\nE. coli Au electrode mannose carbohydrate ligand EIS; Fe(CN)63-/4- 100 CFU/mL Guo et al. (2012)\nS. aureus graphene interdigitated microelectrode array odoranin-HP peptide conductometry 1 × 104 cells/mL Mannoor et al. (2012)\nHelicobacter pylori graphene interdigitated microelectrode array odoranin-HP peptide conductometry 100 cells Mannoor et al. (2012)\nL. innocua Au electrode L. innocua-specific bacteriophage EIS; Fe(CN)63-/4- 1.1 × 104 CFU/mL Tolba et al. (2012)\nE. coli polyaniline on Au electrode monoclonal anti-E. coli EIS 100 CFU/mL Chowdhury et al. (2012).\nE. coli Au interdigitated microelectrode array anti-E. coli EIS 2.5 × 104 CFU/mL Dweik et al. (2012).\nE. coli ultra-nanocrystalline diamond microelectrode array anti-E. coli EIS; Fe(CN)63-/4- 1 × 103 CFU/mL Siddiqui et al. (2012).\nhuman influenza A virus H1N1 Au microelectrode phenotype-specific sialic acid-galactose moieties EIS; Fe(CN)63-/4- – Wicklein et al. (2013)\nE. coli Au electrode E. coli-specific bacteriophages EIS; Fe(CN)63-/4- 800 CFU/mL Tlili et al. (2013)\nDENV-2, dengue virus 3 (DENV-3) Pt-coated nanostructured alumina membrane electrode monoclonal anti-dengue EIS; Fe(CN)63-/4- 0.23 PFU/mL, 0.71 PFU/mL Peh and Li (2013)\ncucumber mosaic virus (CMV) polypyrrole nanoribbons on Au microelectrode array polyclonal anti-CMV amperometry 10 ng/mL Chartuprayoon et al. (2013)\nE. coli Au electrode polyclonal anti-E. coli EIS; Fe(CN)63- 2 CFU/mL Barreiros dos Santos et al. (2013)\nE. coli AuNPs on reduced graphene oxide microelectrode anti-E. coli EIS; Fe(CN)63-/4- 150 CFU/mL Wang et al. (2013)\nE. coli Ag/AgCl wire electrode anti-E. coli EIS 10 CFU/mL Joung et al. (2013)\nmurine norovirus (MNV) AuNPs on carbon electrode anti-norovirus (MNV) aptamer SWV, fluorescence; Fe(CN)63-/Ru(NH3)63+ 180 viruses Giamberardino et al. (2013)\nrotavirus reduced graphene oxide microelectrode anti-rotavirus amperometry 100 PFU Liu et al. (2013)\nS. typhimurium AuNP-functionalized poly(amidoamine)-CNT-chitosan composite on carbon electrode anti- S. typhimurium CV, EIS; Fe(CN)63-/4- 500 CFU/mL Dong et al. (2013)\nE. coli Au-tungsten microwire electrode monoclonal anti-E. coli EIS; Fe(CN)63-/4- 5 CFU/mL Lu et al. (2013)\nE. coli Pt wire electrode anti-E. coli EIS 10 CFU/mL Chan et al. (2013)\nS. aureus reduced graphene oxide on carbon rod electrode anti-S. aureus aptamer potentiometry 1 CFU/mL Hernandez et al. (2014)\nE. coli PAA/PD/CNT composite on carbon electrode anti-E. coli ASV 13 CFU/mL Chen et al. (2014)\nS. typhimurium AuNPs on graphene oxide on carbon electrode anti-S. typhimurium aptamer EIS; Fe(CN)63-/4- 3 CFU/mL Ma et al. (2014)\nS. aureus AuNPs on reduced graphene oxide on carbon electrode anti-S. aureus synthetic aptamer EIS; Fe(CN)63-/4- 10 CFU/mL Jia et al. (2014)\nE. coli Au electrode mannose carbohydrate ligand CV, mass change 1 CFU/mL Yazgan et al. (2014)\nL. monocytogenes Au interdigitated microelectrode array leucocin A antimicrobial peptide EIS 103 CFU/mL Etayash et al. (2014)\nS. typhimurium Au interdigitated microelectrode array monoclonal anti-S. typhimurium EIS 3 × 103 CFU/mL Dastider et al. (2015)\nS. aureus Au electrode polyclonal anti-S. typhimurium EIS; Fe(CN)63-/4- 10 CFU/mL Bekir et al. (2015)\nE. coli CNTs on Au electrode clavanin A peptide EIS; Fe(CN)63-/4- 100 CFU/mL Andrade et al. (2015)\nKlebsiella pneumoniae CNTs on Au electrode clavanin A peptide EIS; Fe(CN)63-/4- 103 CFU/mL Andrade et al. (2015)\nEnterococcus faecalis CNTs on Au electrode clavanin A peptide EIS; Fe(CN)63-/4- 103 CFU/mL Andrade et al. (2015)\nB. subtilis CNTs on Au electrode clavanin A peptide EIS; Fe(CN)63-/4- 100 CFU/mL Andrade et al. (2015)\nE. coli PEI/CNT composite on carbon electrode E. coli-specific bacteriophages EIS; Fe(CN)63-/4- 50 CFU/mL Zhou and Ramasamy (2015)\ndengue virus 1–4 AuNPs on Au electrode anti-DENV-1, anti-DENV-2, anti-DENV-3, anti-DENV-4 CV, EIS; Fe(CN)63-/4- – Luna et al. (2015)\nE. coli ITO microelectrode monoclonal anti-E. coli EIS; Fe(CN)63-/4- 1 CFU/mL Barreiros dos Santos et al. (2015)\navian influenza virus (AIV) H5N1 Au interdigitated microelectrode array monoclonal anti-AIV-H5N1 EIS; Fe(CN)63-/4- 4 HAU/mL Lin et al. (2015)\nC. parvum AuNPs on carbon electrode anti-C. parvum aptamer SWV; Fe(CN)63-/4- 100 oocysts Iqbal et al. (2015)\nE. coli CNT-coated Au-tungsten microwire electrodes polyclonal anti-E. coli amperometry 100 CFU/mL Yamada et al. (2016)\nS. aureus CNT-coated Au-tungsten microwire electrodes polyclonal anti-S. aureus amperometry 100 CFU/mL Yamada et al. (2016)\nS. aureus Au interdigitated microelectrode array anti-S. aureus EIS; Fe(CN)63-/4- 1.3 CFU/mL Primiceri et al. (2016)\nL. monocytogenes Au interdigitated microelectrode array anti-L. monocytogenes EIS; Fe(CN)63-/4- 5 CFU/mL Primiceri et al. (2016)\nnorovirus Au microelectrode anti-norovirus aptamer SWV; Fe(CN)63-/Ru(NH3)63+ 10 PFU/mL Kitajima et al. (2016)\navian influenza virus (AIV) H5N1 Au interdigitated microelectrode array anti-AIV-H5N1 aptamer EIS; Fe(CN)63-/4- 4.2 HAU/mL Callaway et al. (2016)\nS. typhimurium poly[pyrrole-co-3-carboxyl-pyrrole] copolymer electrode anti-S. typhimurium aptamer EIS 3 CFU/mL Sheikhzadeh et al. (2016)\nE. coli polysilicon interdigitated microelectrodes polyclonal anti-E. coli EIS – Mallén-Alberdi et al. (2016)\nhuman influenza A virus H3N2 Au electrode phenotype-specific oligoethylene glycol moieties EIS 1.3 × 104 viruses/mL Hushegyi et al. (2016)\nE. coli PEI/CNT composite on Au microwire electrode polyclonal anti-E. coli amperometry 100 CFU/mL Lee and Jun (2016)\nV. cholerae CeO2 nanowires on Pt microelectrode anti-V. cholerae EIS; Fe(CN)63-/4- 100 CFU/mL Tam and Thang (2016)\nS. aureus PEI/CNT composite on Au microwire electrode polyclonal anti-S. aureus amperometry 100 CFU/mL Lee and Jun (2016)\nE. coli graphene microelectrode polyclonal anti-E. coli amperometry 5 × 103 CFU/mL Wu et al. (2016)\nE. coli Au electrode concanavalin A lectin EIS; Fe(CN)63-/4- 75 cells/mL Yang et al. (2016b)\nE. coli Pt wire electrodes anti-E. coli EIS 100 CFU/mL Tian et al. (2016)\nS. aureus Pt wire electrodes anti-S. aureus EIS 100 CFU/mL Tian et al. (2016)\nB. subtilis CNTs on Au interdigitated microelectrode array polyclonal anti-B. subtilis conductometry 100 CFU/mL Yoo et al. (2017)\nS. epidermidis Au microelectrode S. epidermidis-imprinted poly(3-aminophenylboronic acid) polymer film EIS; Fe(CN)63-/4- 103 CFU/mL Golabi et al. (2017)\nnorovirus graphene/AuNP composite on carbon electrode anti-norovirus aptamer DPV; Ferrocene 100 pM Chand and Neethirajan (2017)\nnorovirus Au electrode synthetic norovirus-specific peptide CV, EIS; Fe(CN)63-/4- 7.8 copies/mL Hwang et al. (2017)\nE. coli CuO/cysteine/reduced graphene/Au oxide electrode monoclonal anti-E. coli EIS; Fe(CN)63-/4- 3.8 CFU/mL Pandey et al. (2017)\nJapanese encephalitis virus (JEV) carbon NPs on carbon electrode monoclonal anti-JEV CV, EIS; Fe(CN)63-/4- 2 ng/mL Chin et al. (2017)\nS. aureus CNTs on carbon electrode polyclonal anti-S. aureus DPV; Fe(CN)63-/4- 13 CFU/mL Bhardwaj et al. (2017)\nhuman influenza A virus H1N1 PEDOT film electrode hemagglutinin-specific trisaccharide ligand EIS, potentiometry, mass change; Fe(CN)63-/4- 0.013 HAU Hai et al. (2017)\nhuman influenza A virus H1N1 reduced graphene oxide on Au microelectrode monoclonal anti-H1N1 chrono-amperometry; Fe(CN)63-/4- 0.5 PFU/mL Singh et al. (2017b)\nE. coli Au microelectrode E. coli-imprinted MAH/HEMA polymer film capacitive 70 CFU/mL Idil et al. (2017)\nE. coli chitosan/polypyrrole/CNT/AuNP composite on graphite electrode monoclonal coli CV; Fe(CN)63-/4- 30 CFU/mL Güner et al. (2017)\nS. dysenteriae AuNPs on carbon electrode anti-S. dysenteriae aptamer EIS; Fe(CN)63-/4- 1 CFU/mL Zarei et al. (2018)\nhuman influenza A virus H1N1 PEDOT:PSS film electrode hemagglutinin-specific trisaccharide ligand amperometry 0.015 HAU Hai et al. (2018)\nS. aureus fluoride-doped tin oxide electrode S. aureus-imprinted Ag–MnO2 film DPV; Fe(CN)63-/4- 103 CFU/mL Divagar et al. (2019)\nE. coli Au microelectrode E. coli-imprinted TEOS/MTMS sol-gel film EIS; Fe(CN)63-/4- 1 CFU/mL Jafari et al. (2019)\nnorovirus Au electrode norovirus-specific peptide EIS; Fe(CN)63-/4- 1.7 copies/mL Baek et al. (2019)\nC. parvum Au interdigitated microelectrode array monoclonal anti-C. parvum Capacitive; Fe(CN)63-/4- 40 cells/mm2 Luka et al. (2019)\nE. coli 4-(3-pyrrol) butryic acid electrode concanavalin A lectin, Arachis hypogaea lectin EIS 6 × 103 CFU/mL Saucedo et al. (2019)\nB. subtilis 4-(3-pyrrol) butryic acid electrode concanavalin A lectin, Arachis hypogaea lectin EIS 6 × 103 CFU/mL Saucedo et al. (2019)\nE. coli silica NPs on polyelectrolyte multilayer on Au electrode polyclonal anti-E. coli CV; Fe(CN)63-/4- 2 × 103 CFU/mL Mathelie-Guinlet et al. (2019)\nE. coli silica NPs on polyelectrolyte multilayer on Au electrode polyclonal anti-E. coli CV; Fe(CN)63-/4- 2 × 103 CFU/mL Mathelie-Guinlet et al. 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