PMC:7143804 / 55174-55689 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7143804","sourcedb":"PMC","sourceid":"7143804","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7143804","text":"Appendix E. Background Signal Fluorescence Measurements\nAll fluorescence measurements in the Horiba Scientific FluoroMax+ spectrofluorometer are normalized by subtracting the background signal. This background signal is measured using the mixture in Table A4.\nTable A4 Mixture used for background signal measurement.\nSample Reaction Buffer Sample Buffer DNA MilliQ DI Water EG Enzyme\n[μL] [μL] [μL] [μL] [μL] [μL]\nBackground 9 9 1 1 4 -\nFigure A4 The background signal of the mixture in Table A4.","divisions":[{"label":"title","span":{"begin":0,"end":55}},{"label":"p","span":{"begin":56,"end":259}},{"label":"table-wrap","span":{"begin":260,"end":454}},{"label":"label","span":{"begin":260,"end":268}},{"label":"caption","span":{"begin":270,"end":317}},{"label":"p","span":{"begin":270,"end":317}},{"label":"table","span":{"begin":318,"end":454}},{"label":"tr","span":{"begin":318,"end":390}},{"label":"th","span":{"begin":318,"end":324}},{"label":"th","span":{"begin":326,"end":341}},{"label":"th","span":{"begin":343,"end":356}},{"label":"th","span":{"begin":358,"end":361}},{"label":"th","span":{"begin":363,"end":378}},{"label":"th","span":{"begin":380,"end":382}},{"label":"th","span":{"begin":384,"end":390}},{"label":"tr","span":{"begin":391,"end":425}},{"label":"td","span":{"begin":391,"end":395}},{"label":"td","span":{"begin":397,"end":401}},{"label":"td","span":{"begin":403,"end":407}},{"label":"td","span":{"begin":409,"end":413}},{"label":"td","span":{"begin":415,"end":419}},{"label":"td","span":{"begin":421,"end":425}},{"label":"tr","span":{"begin":426,"end":454}},{"label":"td","span":{"begin":426,"end":436}},{"label":"td","span":{"begin":438,"end":439}},{"label":"td","span":{"begin":441,"end":442}},{"label":"td","span":{"begin":444,"end":445}},{"label":"td","span":{"begin":447,"end":448}},{"label":"td","span":{"begin":450,"end":451}},{"label":"td","span":{"begin":453,"end":454}},{"label":"label","span":{"begin":455,"end":464}}],"tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"363","span":{"begin":392,"end":394},"obj":"Gene"},{"id":"364","span":{"begin":422,"end":424},"obj":"Gene"},{"id":"365","span":{"begin":416,"end":418},"obj":"Gene"},{"id":"366","span":{"begin":410,"end":412},"obj":"Gene"},{"id":"367","span":{"begin":404,"end":406},"obj":"Gene"},{"id":"368","span":{"begin":398,"end":400},"obj":"Gene"},{"id":"369","span":{"begin":373,"end":378},"obj":"Chemical"}],"attributes":[{"id":"A363","pred":"tao:has_database_id","subj":"363","obj":"Gene:4591"},{"id":"A364","pred":"tao:has_database_id","subj":"364","obj":"Gene:4591"},{"id":"A365","pred":"tao:has_database_id","subj":"365","obj":"Gene:4591"},{"id":"A366","pred":"tao:has_database_id","subj":"366","obj":"Gene:4591"},{"id":"A367","pred":"tao:has_database_id","subj":"367","obj":"Gene:4591"},{"id":"A368","pred":"tao:has_database_id","subj":"368","obj":"Gene:4591"},{"id":"A369","pred":"tao:has_database_id","subj":"369","obj":"MESH:D014867"},{"subj":"363","pred":"source","obj":"LitCovid-PubTator"},{"subj":"364","pred":"source","obj":"LitCovid-PubTator"},{"subj":"365","pred":"source","obj":"LitCovid-PubTator"},{"subj":"366","pred":"source","obj":"LitCovid-PubTator"},{"subj":"367","pred":"source","obj":"LitCovid-PubTator"},{"subj":"368","pred":"source","obj":"LitCovid-PubTator"},{"subj":"369","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T136","span":{"begin":0,"end":8},"obj":"Body_part"},{"id":"T137","span":{"begin":358,"end":361},"obj":"Body_part"}],"attributes":[{"id":"A136","pred":"fma_id","subj":"T136","obj":"http://purl.org/sig/ont/fma/fma14542"},{"id":"A137","pred":"fma_id","subj":"T137","obj":"http://purl.org/sig/ont/fma/fma74412"},{"subj":"T136","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T137","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T48","span":{"begin":370,"end":372},"obj":"Disease"}],"attributes":[{"id":"A48","pred":"mondo_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/MONDO_0018849"},{"subj":"T48","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T418","span":{"begin":23,"end":29},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T419","span":{"begin":186,"end":192},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T420","span":{"begin":210,"end":216},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T421","span":{"begin":298,"end":304},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T422","span":{"begin":444,"end":451},"obj":"http://purl.obolibrary.org/obo/CLO_0053001"},{"id":"T423","span":{"begin":481,"end":487},"obj":"http://purl.obolibrary.org/obo/SO_0000418"}],"attributes":[{"subj":"T418","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T419","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T420","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T421","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T422","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T423","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T14215","span":{"begin":239,"end":246},"obj":"Chemical"},{"id":"T66121","span":{"begin":358,"end":361},"obj":"Chemical"},{"id":"T24099","span":{"begin":373,"end":378},"obj":"Chemical"},{"id":"T80499","span":{"begin":380,"end":382},"obj":"Chemical"},{"id":"T90409","span":{"begin":495,"end":502},"obj":"Chemical"}],"attributes":[{"id":"A34456","pred":"chebi_id","subj":"T14215","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"},{"id":"A13245","pred":"chebi_id","subj":"T66121","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A7320","pred":"chebi_id","subj":"T24099","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A89478","pred":"chebi_id","subj":"T80499","obj":"http://purl.obolibrary.org/obo/CHEBI_73505"},{"id":"A85111","pred":"chebi_id","subj":"T90409","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"},{"subj":"T14215","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T66121","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T24099","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T80499","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T90409","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T491","span":{"begin":0,"end":11},"obj":"Sentence"},{"id":"T492","span":{"begin":12,"end":55},"obj":"Sentence"},{"id":"T493","span":{"begin":56,"end":193},"obj":"Sentence"},{"id":"T494","span":{"begin":194,"end":259},"obj":"Sentence"},{"id":"T495","span":{"begin":260,"end":317},"obj":"Sentence"},{"id":"T496","span":{"begin":318,"end":390},"obj":"Sentence"},{"id":"T497","span":{"begin":391,"end":425},"obj":"Sentence"},{"id":"T498","span":{"begin":426,"end":454},"obj":"Sentence"},{"id":"T499","span":{"begin":455,"end":515},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T491","pred":"source","obj":"LitCovid-sentences"},{"subj":"T492","pred":"source","obj":"LitCovid-sentences"},{"subj":"T493","pred":"source","obj":"LitCovid-sentences"},{"subj":"T494","pred":"source","obj":"LitCovid-sentences"},{"subj":"T495","pred":"source","obj":"LitCovid-sentences"},{"subj":"T496","pred":"source","obj":"LitCovid-sentences"},{"subj":"T497","pred":"source","obj":"LitCovid-sentences"},{"subj":"T498","pred":"source","obj":"LitCovid-sentences"},{"subj":"T499","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#a693ec","default":true},{"id":"LitCovid-PD-FMA-UBERON","color":"#99ec93"},{"id":"LitCovid-PD-MONDO","color":"#ec93b3"},{"id":"LitCovid-PD-CLO","color":"#93cdec"},{"id":"LitCovid-PD-CHEBI","color":"#e7ec93"},{"id":"LitCovid-sentences","color":"#d793ec"}]}]}}