Appendix E. Background Signal Fluorescence Measurements
All fluorescence measurements in the Horiba Scientific FluoroMax+ spectrofluorometer are normalized by subtracting the background signal. This background signal is measured using the mixture in Table A4.
Table A4  Mixture used for background signal measurement.
Sample  Reaction Buffer  Sample Buffer  DNA  MilliQ DI Water  EG  Enzyme
[μL]  [μL]  [μL]  [μL]  [μL]  [μL]
Background  9  9  1  1  4  -
Figure A4  The background signal of the mixture in Table A4.