PMC:7143804 / 45453-47007
Annnotations
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 303 | 274-282 | Gene | denotes | helicase | Gene:164045 |
| 304 | 87-95 | Disease | denotes | zoonoses | MESH:D015047 |
| 305 | 1230-1247 | Disease | denotes | zoonoses diseases | MESH:D015047 |
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T82 | 170-173 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T83 | 270-273 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T84 | 320-323 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T85 | 424-432 | Body_part | denotes | proteins | http://purl.org/sig/ont/fma/fma67257 |
| T86 | 562-565 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T87 | 985-988 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T88 | 1056-1059 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T89 | 1134-1137 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
| T90 | 1184-1187 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T36 | 87-95 | Disease | denotes | zoonoses | http://purl.obolibrary.org/obo/MONDO_0025481 |
| T37 | 1230-1238 | Disease | denotes | zoonoses | http://purl.obolibrary.org/obo/MONDO_0025481 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T309 | 35-36 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T310 | 37-43 | http://purl.obolibrary.org/obo/OBI_0000968 | denotes | device |
| T311 | 141-147 | http://purl.obolibrary.org/obo/OBI_0000968 | denotes | device |
| T312 | 560-561 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T313 | 646-647 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T314 | 901-902 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T315 | 983-984 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T316 | 1061-1063 | http://purl.obolibrary.org/obo/CLO_0053733 | denotes | 11 |
| T317 | 1132-1133 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T318 | 1199-1206 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | viruses |
| T319 | 1208-1210 | http://purl.obolibrary.org/obo/CLO_0053733 | denotes | 11 |
| T320 | 1218-1223 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
| T321 | 1262-1269 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | viruses |
| T322 | 1271-1273 | http://purl.obolibrary.org/obo/CLO_0050507 | denotes | 22 |
| T323 | 1294-1295 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T324 | 1400-1401 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T325 | 1473-1474 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T305 | 170-173 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T306 | 244-247 | Chemical | denotes | HDA | http://purl.obolibrary.org/obo/CHEBI_17314 |
| T307 | 257-260 | Chemical | denotes | HDA | http://purl.obolibrary.org/obo/CHEBI_17314 |
| T308 | 270-273 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T309 | 320-323 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T310 | 424-432 | Chemical | denotes | proteins | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T311 | 562-565 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T312 | 624-629 | Chemical | denotes | bases | http://purl.obolibrary.org/obo/CHEBI_22695 |
| T313 | 825-828 | Chemical | denotes | MDA | http://purl.obolibrary.org/obo/CHEBI_566274 |
| T314 | 985-988 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T315 | 1056-1059 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T316 | 1134-1137 | Chemical | denotes | RNA | http://purl.obolibrary.org/obo/CHEBI_33697 |
| T317 | 1184-1187 | Chemical | denotes | RNA | http://purl.obolibrary.org/obo/CHEBI_33697 |
| T318 | 1255-1261 | Chemical | denotes | corona | http://purl.obolibrary.org/obo/CHEBI_37409 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T20 | 170-188 | http://purl.obolibrary.org/obo/GO_0006277 | denotes | DNA amplifications |
| T21 | 320-335 | http://purl.obolibrary.org/obo/GO_0006260 | denotes | DNA replication |
| T22 | 1104-1117 | http://purl.obolibrary.org/obo/GO_0003968 | denotes | transcriptase |
| T23 | 1104-1117 | http://purl.obolibrary.org/obo/GO_0003899 | denotes | transcriptase |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T357 | 0-256 | Sentence | denotes | Future steps, which will result in a device for early-stage detection of, for example, zoonoses, include studies on the optimization of this device for sequence specific DNA amplifications (e.g., primer design and reaction optimization), i.e., HDA or LAMP. |
| T358 | 257-391 | Sentence | denotes | HDA utilizes DNA helicase (an enzyme also used in vitro during DNA replication) to separate the dsDNA instead of thermal denaturation. |
| T359 | 392-630 | Sentence | denotes | After separation, ssDNA binding proteins hybridize on the ssDNA strands for stabilization, ensuring that the next primer will have time to bind to the ssDNA stripe and a DNA polymerase will extend the primers with the complementary bases. |
| T360 | 631-800 | Sentence | denotes | This method is a truly isothermal technique in which the separation of the dsDNA can be performed at the same temperature as the amplification reaction, i.e., 64 °C [9]. |
| T361 | 801-880 | Sentence | denotes | LAMP is more similar to MDA in the way it also uses heat to denature the dsDNA. |
| T362 | 881-1065 | Sentence | denotes | After denaturation, a set of four primers (six can be used as well to achieve better selectivity) and a DNA polymerase is used at isothermal conditions (65 °C) to amplify the DNA [11]. |
| T363 | 1066-1275 | Sentence | denotes | When used in combination with reverse transcriptase, LAMP becomes a RNA amplification method, which could be used for RNA-containing viruses [11], like virus-based zoonoses diseases as the corona viruses [22]. |
| T364 | 1276-1394 | Sentence | denotes | Despite not being a truly isothermal technique, LAMP offers the possibility to use turbidity as detection method [76]. |
| T365 | 1395-1554 | Sentence | denotes | Such a detection method would simplify the required equipment even further as a decrease in transmitted light through the chip can be used as detection method. |
2_test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 32106462-15247927-69893327 | 797-798 | 15247927 | denotes | 9 |
| 32106462-21157218-69893328 | 1061-1063 | 21157218 | denotes | 11 |
| 32106462-21157218-69893329 | 1208-1210 | 21157218 | denotes | 11 |
| 32106462-21479543-69893330 | 1271-1273 | 21479543 | denotes | 22 |
| 32106462-11708792-69893331 | 1390-1392 | 11708792 | denotes | 76 |