PMC:7143804 / 44019-45135 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T80","span":{"begin":47,"end":50},"obj":"Body_part"},{"id":"T81","span":{"begin":301,"end":304},"obj":"Body_part"}],"attributes":[{"id":"A80","pred":"fma_id","subj":"T80","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A81","pred":"fma_id","subj":"T81","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"With the proof-of-principle device, successful DNA amplifications using MDA inside a disposable polymeric chip were achieved. The heat for the reaction was applied using the integrated low-cost Au-resistive heater. The device was operated at a suitable temperature for MDA reactions and the amplified DNA was measured using EvaGreen fluorescence dye and an ex situ spectrofluorometer. A distinct peak is visible in the reaction mixtures which is absent in the NTC mixtures. The operating temperature for MDA reactions is around 30 °C, which is comparable with a nice summer day. Using amplification reactions which such low reaction temperatures could encounter problems at warmer locations. However, as MDA is not sequence specific, this reaction will not be integrated in the final protocols. MDA was only used as proof-of-principle reaction to show the biocompatibility of the device and functioning of the integrated heater. Sequence specific amplifications, e.g., HDA and LAMP, are performed at higher temperatures, as will be discussed in Section 5. This makes the system less sensitive to the hot summer days."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T299","span":{"begin":28,"end":34},"obj":"http://purl.obolibrary.org/obo/OBI_0000968"},{"id":"T300","span":{"begin":83,"end":84},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T301","span":{"begin":219,"end":225},"obj":"http://purl.obolibrary.org/obo/OBI_0000968"},{"id":"T302","span":{"begin":242,"end":243},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T303","span":{"begin":385,"end":386},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T304","span":{"begin":560,"end":561},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T305","span":{"begin":880,"end":886},"obj":"http://purl.obolibrary.org/obo/OBI_0000968"}],"text":"With the proof-of-principle device, successful DNA amplifications using MDA inside a disposable polymeric chip were achieved. The heat for the reaction was applied using the integrated low-cost Au-resistive heater. The device was operated at a suitable temperature for MDA reactions and the amplified DNA was measured using EvaGreen fluorescence dye and an ex situ spectrofluorometer. A distinct peak is visible in the reaction mixtures which is absent in the NTC mixtures. The operating temperature for MDA reactions is around 30 °C, which is comparable with a nice summer day. Using amplification reactions which such low reaction temperatures could encounter problems at warmer locations. However, as MDA is not sequence specific, this reaction will not be integrated in the final protocols. MDA was only used as proof-of-principle reaction to show the biocompatibility of the device and functioning of the integrated heater. Sequence specific amplifications, e.g., HDA and LAMP, are performed at higher temperatures, as will be discussed in Section 5. This makes the system less sensitive to the hot summer days."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T295","span":{"begin":47,"end":50},"obj":"Chemical"},{"id":"T296","span":{"begin":72,"end":75},"obj":"Chemical"},{"id":"T297","span":{"begin":194,"end":196},"obj":"Chemical"},{"id":"T298","span":{"begin":269,"end":272},"obj":"Chemical"},{"id":"T299","span":{"begin":301,"end":304},"obj":"Chemical"},{"id":"T300","span":{"begin":346,"end":349},"obj":"Chemical"},{"id":"T301","span":{"begin":504,"end":507},"obj":"Chemical"},{"id":"T302","span":{"begin":704,"end":707},"obj":"Chemical"},{"id":"T303","span":{"begin":795,"end":798},"obj":"Chemical"},{"id":"T304","span":{"begin":969,"end":972},"obj":"Chemical"}],"attributes":[{"id":"A295","pred":"chebi_id","subj":"T295","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A296","pred":"chebi_id","subj":"T296","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"},{"id":"A297","pred":"chebi_id","subj":"T297","obj":"http://purl.obolibrary.org/obo/CHEBI_29287"},{"id":"A298","pred":"chebi_id","subj":"T298","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"},{"id":"A299","pred":"chebi_id","subj":"T299","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A300","pred":"chebi_id","subj":"T300","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A301","pred":"chebi_id","subj":"T301","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"},{"id":"A302","pred":"chebi_id","subj":"T302","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"},{"id":"A303","pred":"chebi_id","subj":"T303","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"},{"id":"A304","pred":"chebi_id","subj":"T304","obj":"http://purl.obolibrary.org/obo/CHEBI_17314"}],"text":"With the proof-of-principle device, successful DNA amplifications using MDA inside a disposable polymeric chip were achieved. The heat for the reaction was applied using the integrated low-cost Au-resistive heater. The device was operated at a suitable temperature for MDA reactions and the amplified DNA was measured using EvaGreen fluorescence dye and an ex situ spectrofluorometer. A distinct peak is visible in the reaction mixtures which is absent in the NTC mixtures. The operating temperature for MDA reactions is around 30 °C, which is comparable with a nice summer day. Using amplification reactions which such low reaction temperatures could encounter problems at warmer locations. However, as MDA is not sequence specific, this reaction will not be integrated in the final protocols. MDA was only used as proof-of-principle reaction to show the biocompatibility of the device and functioning of the integrated heater. Sequence specific amplifications, e.g., HDA and LAMP, are performed at higher temperatures, as will be discussed in Section 5. This makes the system less sensitive to the hot summer days."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T19","span":{"begin":47,"end":65},"obj":"http://purl.obolibrary.org/obo/GO_0006277"}],"text":"With the proof-of-principle device, successful DNA amplifications using MDA inside a disposable polymeric chip were achieved. The heat for the reaction was applied using the integrated low-cost Au-resistive heater. The device was operated at a suitable temperature for MDA reactions and the amplified DNA was measured using EvaGreen fluorescence dye and an ex situ spectrofluorometer. A distinct peak is visible in the reaction mixtures which is absent in the NTC mixtures. The operating temperature for MDA reactions is around 30 °C, which is comparable with a nice summer day. Using amplification reactions which such low reaction temperatures could encounter problems at warmer locations. However, as MDA is not sequence specific, this reaction will not be integrated in the final protocols. MDA was only used as proof-of-principle reaction to show the biocompatibility of the device and functioning of the integrated heater. Sequence specific amplifications, e.g., HDA and LAMP, are performed at higher temperatures, as will be discussed in Section 5. This makes the system less sensitive to the hot summer days."}

{"project":"LitCovid-sentences","denotations":[{"id":"T342","span":{"begin":0,"end":125},"obj":"Sentence"},{"id":"T343","span":{"begin":126,"end":214},"obj":"Sentence"},{"id":"T344","span":{"begin":215,"end":384},"obj":"Sentence"},{"id":"T345","span":{"begin":385,"end":473},"obj":"Sentence"},{"id":"T346","span":{"begin":474,"end":578},"obj":"Sentence"},{"id":"T347","span":{"begin":579,"end":691},"obj":"Sentence"},{"id":"T348","span":{"begin":692,"end":794},"obj":"Sentence"},{"id":"T349","span":{"begin":795,"end":928},"obj":"Sentence"},{"id":"T350","span":{"begin":929,"end":1055},"obj":"Sentence"},{"id":"T351","span":{"begin":1056,"end":1116},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"With the proof-of-principle device, successful DNA amplifications using MDA inside a disposable polymeric chip were achieved. The heat for the reaction was applied using the integrated low-cost Au-resistive heater. The device was operated at a suitable temperature for MDA reactions and the amplified DNA was measured using EvaGreen fluorescence dye and an ex situ spectrofluorometer. A distinct peak is visible in the reaction mixtures which is absent in the NTC mixtures. The operating temperature for MDA reactions is around 30 °C, which is comparable with a nice summer day. Using amplification reactions which such low reaction temperatures could encounter problems at warmer locations. However, as MDA is not sequence specific, this reaction will not be integrated in the final protocols. MDA was only used as proof-of-principle reaction to show the biocompatibility of the device and functioning of the integrated heater. Sequence specific amplifications, e.g., HDA and LAMP, are performed at higher temperatures, as will be discussed in Section 5. This makes the system less sensitive to the hot summer days."}