PMC:7143804 / 44019-45135
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T80 | 47-50 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T81 | 301-304 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T299 | 28-34 | http://purl.obolibrary.org/obo/OBI_0000968 | denotes | device |
| T300 | 83-84 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T301 | 219-225 | http://purl.obolibrary.org/obo/OBI_0000968 | denotes | device |
| T302 | 242-243 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T303 | 385-386 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | A |
| T304 | 560-561 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T305 | 880-886 | http://purl.obolibrary.org/obo/OBI_0000968 | denotes | device |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T295 | 47-50 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T296 | 72-75 | Chemical | denotes | MDA | http://purl.obolibrary.org/obo/CHEBI_566274 |
| T297 | 194-196 | Chemical | denotes | Au | http://purl.obolibrary.org/obo/CHEBI_29287 |
| T298 | 269-272 | Chemical | denotes | MDA | http://purl.obolibrary.org/obo/CHEBI_566274 |
| T299 | 301-304 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T300 | 346-349 | Chemical | denotes | dye | http://purl.obolibrary.org/obo/CHEBI_37958 |
| T301 | 504-507 | Chemical | denotes | MDA | http://purl.obolibrary.org/obo/CHEBI_566274 |
| T302 | 704-707 | Chemical | denotes | MDA | http://purl.obolibrary.org/obo/CHEBI_566274 |
| T303 | 795-798 | Chemical | denotes | MDA | http://purl.obolibrary.org/obo/CHEBI_566274 |
| T304 | 969-972 | Chemical | denotes | HDA | http://purl.obolibrary.org/obo/CHEBI_17314 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T19 | 47-65 | http://purl.obolibrary.org/obo/GO_0006277 | denotes | DNA amplifications |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T342 | 0-125 | Sentence | denotes | With the proof-of-principle device, successful DNA amplifications using MDA inside a disposable polymeric chip were achieved. |
| T343 | 126-214 | Sentence | denotes | The heat for the reaction was applied using the integrated low-cost Au-resistive heater. |
| T344 | 215-384 | Sentence | denotes | The device was operated at a suitable temperature for MDA reactions and the amplified DNA was measured using EvaGreen fluorescence dye and an ex situ spectrofluorometer. |
| T345 | 385-473 | Sentence | denotes | A distinct peak is visible in the reaction mixtures which is absent in the NTC mixtures. |
| T346 | 474-578 | Sentence | denotes | The operating temperature for MDA reactions is around 30 °C, which is comparable with a nice summer day. |
| T347 | 579-691 | Sentence | denotes | Using amplification reactions which such low reaction temperatures could encounter problems at warmer locations. |
| T348 | 692-794 | Sentence | denotes | However, as MDA is not sequence specific, this reaction will not be integrated in the final protocols. |
| T349 | 795-928 | Sentence | denotes | MDA was only used as proof-of-principle reaction to show the biocompatibility of the device and functioning of the integrated heater. |
| T350 | 929-1055 | Sentence | denotes | Sequence specific amplifications, e.g., HDA and LAMP, are performed at higher temperatures, as will be discussed in Section 5. |
| T351 | 1056-1116 | Sentence | denotes | This makes the system less sensitive to the hot summer days. |