PMC:7143804 / 41336-42230 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"290","span":{"begin":132,"end":134},"obj":"Gene"},{"id":"291","span":{"begin":145,"end":150},"obj":"Chemical"}],"attributes":[{"id":"A290","pred":"tao:has_database_id","subj":"290","obj":"Gene:4591"},{"id":"A291","pred":"tao:has_database_id","subj":"291","obj":"MESH:D014867"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA). Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification. The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]). The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA. No Enzyme was added to this mixture, therefore no amplification could take place. See Figure 15 for the results obtained in the Eppendorf tube and chips. Figure A4 in Appendix E shows the background signal which is subtracted from all measurements."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T71","span":{"begin":641,"end":644},"obj":"Body_part"},{"id":"T72","span":{"begin":813,"end":821},"obj":"Body_part"}],"attributes":[{"id":"A71","pred":"fma_id","subj":"T71","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A72","pred":"fma_id","subj":"T72","obj":"http://purl.org/sig/ont/fma/fma14542"}],"text":"After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA). Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification. The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]). The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA. No Enzyme was added to this mixture, therefore no amplification could take place. See Figure 15 for the results obtained in the Eppendorf tube and chips. Figure A4 in Appendix E shows the background signal which is subtracted from all measurements."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T13","span":{"begin":784,"end":788},"obj":"Body_part"}],"attributes":[{"id":"A13","pred":"uberon_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/UBERON_0000025"}],"text":"After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA). Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification. The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]). The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA. No Enzyme was added to this mixture, therefore no amplification could take place. See Figure 15 for the results obtained in the Eppendorf tube and chips. Figure A4 in Appendix E shows the background signal which is subtracted from all measurements."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T35","span":{"begin":142,"end":144},"obj":"Disease"}],"attributes":[{"id":"A35","pred":"mondo_id","subj":"T35","obj":"http://purl.obolibrary.org/obo/MONDO_0018849"}],"text":"After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA). Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification. The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]). The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA. No Enzyme was added to this mixture, therefore no amplification could take place. See Figure 15 for the results obtained in the Eppendorf tube and chips. Figure A4 in Appendix E shows the background signal which is subtracted from all measurements."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T282","span":{"begin":82,"end":87},"obj":"http://purl.obolibrary.org/obo/UBERON_0000025"},{"id":"T283","span":{"begin":229,"end":230},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T284","span":{"begin":337,"end":338},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T285","span":{"begin":457,"end":460},"obj":"http://purl.obolibrary.org/obo/CLO_0051582"},{"id":"T286","span":{"begin":461,"end":462},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T287","span":{"begin":551,"end":557},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T288","span":{"begin":561,"end":562},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T289","span":{"begin":784,"end":788},"obj":"http://purl.obolibrary.org/obo/UBERON_0000025"},{"id":"T290","span":{"begin":845,"end":851},"obj":"http://purl.obolibrary.org/obo/SO_0000418"}],"text":"After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA). Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification. The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]). The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA. No Enzyme was added to this mixture, therefore no amplification could take place. See Figure 15 for the results obtained in the Eppendorf tube and chips. Figure A4 in Appendix E shows the background signal which is subtracted from all measurements."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T267","span":{"begin":102,"end":108},"obj":"Chemical"},{"id":"T268","span":{"begin":145,"end":150},"obj":"Chemical"},{"id":"T269","span":{"begin":181,"end":183},"obj":"Chemical"},{"id":"T272","span":{"begin":313,"end":320},"obj":"Chemical"},{"id":"T273","span":{"begin":453,"end":456},"obj":"Chemical"},{"id":"T274","span":{"begin":563,"end":570},"obj":"Chemical"},{"id":"T275","span":{"begin":600,"end":606},"obj":"Chemical"},{"id":"T276","span":{"begin":619,"end":625},"obj":"Chemical"},{"id":"T277","span":{"begin":641,"end":644},"obj":"Chemical"},{"id":"T278","span":{"begin":674,"end":681},"obj":"Chemical"}],"attributes":[{"id":"A267","pred":"chebi_id","subj":"T267","obj":"http://purl.obolibrary.org/obo/CHEBI_46727"},{"id":"A268","pred":"chebi_id","subj":"T268","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A269","pred":"chebi_id","subj":"T269","obj":"http://purl.obolibrary.org/obo/CHEBI_474859"},{"id":"A270","pred":"chebi_id","subj":"T269","obj":"http://purl.obolibrary.org/obo/CHEBI_73610"},{"id":"A271","pred":"chebi_id","subj":"T269","obj":"http://purl.obolibrary.org/obo/CHEBI_90325"},{"id":"A272","pred":"chebi_id","subj":"T272","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"},{"id":"A273","pred":"chebi_id","subj":"T273","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A274","pred":"chebi_id","subj":"T274","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"},{"id":"A275","pred":"chebi_id","subj":"T275","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A276","pred":"chebi_id","subj":"T276","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A277","pred":"chebi_id","subj":"T277","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A278","pred":"chebi_id","subj":"T278","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"}],"text":"After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA). Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification. The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]). The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA. No Enzyme was added to this mixture, therefore no amplification could take place. See Figure 15 for the results obtained in the Eppendorf tube and chips. Figure A4 in Appendix E shows the background signal which is subtracted from all measurements."}

{"project":"LitCovid-sentences","denotations":[{"id":"T321","span":{"begin":0,"end":190},"obj":"Sentence"},{"id":"T322","span":{"begin":191,"end":308},"obj":"Sentence"},{"id":"T323","span":{"begin":309,"end":484},"obj":"Sentence"},{"id":"T324","span":{"begin":485,"end":645},"obj":"Sentence"},{"id":"T325","span":{"begin":646,"end":727},"obj":"Sentence"},{"id":"T326","span":{"begin":728,"end":799},"obj":"Sentence"},{"id":"T327","span":{"begin":800,"end":894},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA). Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification. The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]). The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA. No Enzyme was added to this mixture, therefore no amplification could take place. See Figure 15 for the results obtained in the Eppendorf tube and chips. Figure A4 in Appendix E shows the background signal which is subtracted from all measurements."}