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PMC:7143804 / 41336-42230 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
290 132-134 Gene denotes μL Gene:4591
291 145-150 Chemical denotes water MESH:D014867

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T71 641-644 Body_part denotes DNA http://purl.org/sig/ont/fma/fma74412
T72 813-821 Body_part denotes Appendix http://purl.org/sig/ont/fma/fma14542

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T13 784-788 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T35 142-144 Disease denotes DI http://purl.obolibrary.org/obo/MONDO_0018849

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T282 82-87 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T283 229-230 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T284 337-338 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T285 457-460 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T286 461-462 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T287 551-557 http://purl.obolibrary.org/obo/SO_0000418 denotes signal
T288 561-562 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T289 784-788 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T290 845-851 http://purl.obolibrary.org/obo/SO_0000418 denotes signal

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T267 102-108 Chemical denotes quartz http://purl.obolibrary.org/obo/CHEBI_46727
T268 145-150 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377
T269 181-183 Chemical denotes MA http://purl.obolibrary.org/obo/CHEBI_474859|http://purl.obolibrary.org/obo/CHEBI_73610|http://purl.obolibrary.org/obo/CHEBI_90325
T272 313-320 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004
T273 453-456 Chemical denotes dye http://purl.obolibrary.org/obo/CHEBI_37958
T274 563-570 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004
T275 600-606 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T276 619-625 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T277 641-644 Chemical denotes DNA http://purl.obolibrary.org/obo/CHEBI_16991
T278 674-681 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T321 0-190 Sentence denotes After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA).
T322 191-308 Sentence denotes Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification.
T323 309-484 Sentence denotes The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]).
T324 485-645 Sentence denotes The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA.
T325 646-727 Sentence denotes No Enzyme was added to this mixture, therefore no amplification could take place.
T326 728-799 Sentence denotes See Figure 15 for the results obtained in the Eppendorf tube and chips.
T327 800-894 Sentence denotes Figure A4 in Appendix E shows the background signal which is subtracted from all measurements.