PMC:7143804 / 39490-40770
Annnotations
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The closed chip and tube are heated up in a separate water bath to 95 °C and kept at that temperature for 3 min to denaturate the dsDNA. Subsequently, the chip and tube are cooled down by placing it in an ice bath for 5 min after which the rest of the reagents are added. The complete mixtures are according to Table A3 in Appendix D. 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