> top > docs > PMC:7143804 > spans > 39490-40770 > annotations

PMC:7143804 / 39490-40770 JSONTXT

Annnotations TAB JSON ListView MergeView

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
277 222-227 Chemical denotes water MESH:D014867
278 323-328 Chemical denotes water MESH:D014867
279 524-532 Chemical denotes EvaGreen
280 825-830 Chemical denotes water MESH:D014867
281 1169-1174 Chemical denotes water MESH:D014867

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T64 69-71 Body_part denotes AG http://purl.org/sig/ont/fma/fma61898
T65 147-149 Body_part denotes V2 http://purl.org/sig/ont/fma/fma13443|http://purl.org/sig/ont/fma/fma68615
T67 150-153 Body_part denotes DNA http://purl.org/sig/ont/fma/fma74412
T68 505-508 Body_part denotes DNA http://purl.org/sig/ont/fma/fma74412
T69 1089-1091 Body_part denotes A3 http://purl.org/sig/ont/fma/fma66599
T70 1095-1103 Body_part denotes Appendix http://purl.org/sig/ont/fma/fma14542

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T7 53-57 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025
T8 448-452 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025
T9 567-571 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025
T10 792-796 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025
T11 936-940 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025
T12 1134-1138 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T266 53-57 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T267 287-289 http://purl.obolibrary.org/obo/UBERON_0003064 denotes im
T268 382-383 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T269 448-452 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T270 567-571 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T271 586-587 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T272 745-746 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T273 792-796 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T274 814-815 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T275 936-940 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T276 1089-1091 http://purl.obolibrary.org/obo/CLO_0001577 denotes A3
T277 1134-1138 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T245 0-3 Chemical denotes MDA http://purl.obolibrary.org/obo/CHEBI_566274
T246 69-71 Chemical denotes AG http://purl.obolibrary.org/obo/CHEBI_27569|http://purl.obolibrary.org/obo/CHEBI_40618|http://purl.obolibrary.org/obo/CHEBI_73757
T249 150-153 Chemical denotes DNA http://purl.obolibrary.org/obo/CHEBI_16991
T250 198-201 Chemical denotes dye http://purl.obolibrary.org/obo/CHEBI_37958
T251 222-227 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377
T252 261-263 Chemical denotes RV http://purl.obolibrary.org/obo/CHEBI_73823
T253 287-289 Chemical denotes im http://purl.obolibrary.org/obo/CHEBI_30366
T254 323-328 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377
T255 482-489 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004
T256 505-508 Chemical denotes DNA http://purl.obolibrary.org/obo/CHEBI_16991
T257 533-536 Chemical denotes dye http://purl.obolibrary.org/obo/CHEBI_37958
T258 537-545 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T259 667-670 Chemical denotes MDA http://purl.obolibrary.org/obo/CHEBI_566274
T260 825-830 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377
T261 1024-1032 Chemical denotes reagents http://purl.obolibrary.org/obo/CHEBI_33893
T262 1169-1174 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T16 150-167 http://purl.obolibrary.org/obo/GO_0006277 denotes DNA amplification

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T305 0-202 Sentence denotes MDA reactions are also performed inside an Eppendorf tube (Eppendorf AG, Hamburg, Germany) and inside the chip, again using the Illustra GenomiPhi V2 DNA amplification kit and EvaGreen fluorescence dye.
T306 203-317 Sentence denotes As heat supply the water bath of an IKA Rotary Evaporator RV 8V (IKA-Werke, Staufen im Breisgau, Germany) is used.
T307 318-421 Sentence denotes This water bath is according to its specification stable within a range of the set temperature ±0.1 °C.
T308 422-546 Sentence denotes The chip and an Eppendorf tube are loaded with the reaction mixture containing the DNA sample and the EvaGreen dye solution.
T309 547-680 Sentence denotes Here, the Eppendorf tube is serving as a control to show that the fabrication steps of the chips are not inhibiting the MDA reaction.
T310 681-771 Sentence denotes The inlet and outlet of the chip are sealed with the Microseal “B” PCR plate sealing foil.
T311 772-908 Sentence denotes The closed chip and tube are heated up in a separate water bath to 95 °C and kept at that temperature for 3 min to denaturate the dsDNA.
T312 909-1043 Sentence denotes Subsequently, the chip and tube are cooled down by placing it in an ice bath for 5 min after which the rest of the reagents are added.
T313 1044-1106 Sentence denotes The complete mixtures are according to Table A3 in Appendix D.
T314 1107-1280 Sentence denotes After closing the chip and tube again, they are placed in the water bath of the rotary evaporater and left there for 90 min, after which the reaction is terminated at 65 °C.