PMC:7120874 / 7045-7951 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7120874","sourcedb":"PMC","sourceid":"7120874","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7120874","text":"Chip Pretreatment, Sample Binding, and Analysis\nCopper (II) immobilized metal affinity capture [IMAC30 Cu(II)] ProteinChip® Array (Ciphergen Biosystems Inc., Fremont, CA).\nWeak cation-exchange (CM10) ProteinChip Array (Ciphergen Biosystems, Inc.).\n96-Well ProteinChip bioprocessor (Ciphergen Biosystems, Inc.).\n100 mM Copper sulfate (CuSO4; Sigma-Adrich, St. Louis, MO).\n100 mM Sodium phosphate containing 0.5 mol/L NaCl (IMAC30 chip binding buffer).\n100 mM Sodium acetate buffer pH 4.0 (serves as both IMAC30 chip neutralizing buffer and CM10 chip binding buffer).\n50% Sinapinic acid (Ciphergen Biosystems Inc.) in 500 mL/L acetonitrile and 5 mL/L trifluoroacetic acid (Sigma-Adrich).\nProtein Biological System (PBS) IIc mass spectrometer reader (Ciphergen Biosystems).\nAll-in-1 peptide molecular mass standard (Ciphergen Biosystems Inc.).\nCiphergen ProteinChip Software 3.0.2 (Ciphergen Biosystems Inc.).","divisions":[{"label":"title","span":{"begin":0,"end":47}},{"label":"p","span":{"begin":48,"end":171}},{"label":"p","span":{"begin":172,"end":247}},{"label":"p","span":{"begin":248,"end":310}},{"label":"p","span":{"begin":311,"end":370}},{"label":"p","span":{"begin":371,"end":450}},{"label":"p","span":{"begin":451,"end":565}},{"label":"p","span":{"begin":566,"end":685}},{"label":"p","span":{"begin":686,"end":770}},{"label":"p","span":{"begin":771,"end":840}}],"tracks":[]}