Chip Pretreatment, Sample Binding, and Analysis
Copper (II) immobilized metal affinity capture [IMAC30 Cu(II)] ProteinChip® Array (Ciphergen Biosystems Inc., Fremont, CA).
Weak cation-exchange (CM10) ProteinChip Array (Ciphergen Biosystems, Inc.).
96-Well ProteinChip bioprocessor (Ciphergen Biosystems, Inc.).
100 mM Copper sulfate (CuSO4; Sigma-Adrich, St. Louis, MO).
100 mM Sodium phosphate containing 0.5 mol/L NaCl (IMAC30 chip binding buffer).
100 mM Sodium acetate buffer pH 4.0 (serves as both IMAC30 chip neutralizing buffer and CM10 chip binding buffer).
50% Sinapinic acid (Ciphergen Biosystems Inc.) in 500 mL/L acetonitrile and 5 mL/L trifluoroacetic acid (Sigma-Adrich).
Protein Biological System (PBS) IIc mass spectrometer reader (Ciphergen Biosystems).
All-in-1 peptide molecular mass standard (Ciphergen Biosystems Inc.).
Ciphergen ProteinChip Software 3.0.2 (Ciphergen Biosystems Inc.).