PMC:7115396 / 41346-42349 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T236","span":{"begin":90,"end":98},"obj":"Body_part"},{"id":"T237","span":{"begin":147,"end":151},"obj":"Body_part"},{"id":"T238","span":{"begin":402,"end":408},"obj":"Body_part"},{"id":"T239","span":{"begin":440,"end":447},"obj":"Body_part"},{"id":"T240","span":{"begin":452,"end":457},"obj":"Body_part"},{"id":"T241","span":{"begin":749,"end":757},"obj":"Body_part"},{"id":"T242","span":{"begin":776,"end":781},"obj":"Body_part"}],"attributes":[{"id":"A236","pred":"fma_id","subj":"T236","obj":"http://purl.org/sig/ont/fma/fma13478"},{"id":"A237","pred":"fma_id","subj":"T237","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A238","pred":"fma_id","subj":"T238","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A239","pred":"fma_id","subj":"T239","obj":"http://purl.org/sig/ont/fma/fma7203"},{"id":"A240","pred":"fma_id","subj":"T240","obj":"http://purl.org/sig/ont/fma/fma68877"},{"id":"A241","pred":"fma_id","subj":"T241","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A242","pred":"fma_id","subj":"T242","obj":"http://purl.org/sig/ont/fma/fma63083"}],"text":"In conclusion, we constructed the recombinant strain, rH120-S1/YZ, which was based on the backbone of the H120 vaccine strain, and replaced the S1 gene with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated from China using the reverse genetic system. The safety results showed that this recombinant strain was not lethal to one-day-old chicks and it had not gained the ability to tissue invasion or replication in the kidneys and lungs of infected animals. Moreover, the viral load of the chickens was significantly decreased compared with the rH120 and rIBYZ strains. Furthermore, rH120-S1/YZ was found to provide effective protection against challenge with the QX-like nephropathogenic strain in young chickens. The level of antibody production in the serum of the vaccinated chickens detected by ELISA continued to increase after 7 dpi. Thus, rH120-S1/YZ may be considered a potential live vaccine candidate for protection against Chinese QX-like nephropathogenic IBV infection."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T82","span":{"begin":402,"end":408},"obj":"Body_part"},{"id":"T83","span":{"begin":776,"end":781},"obj":"Body_part"}],"attributes":[{"id":"A82","pred":"uberon_id","subj":"T82","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A83","pred":"uberon_id","subj":"T83","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"In conclusion, we constructed the recombinant strain, rH120-S1/YZ, which was based on the backbone of the H120 vaccine strain, and replaced the S1 gene with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated from China using the reverse genetic system. The safety results showed that this recombinant strain was not lethal to one-day-old chicks and it had not gained the ability to tissue invasion or replication in the kidneys and lungs of infected animals. Moreover, the viral load of the chickens was significantly decreased compared with the rH120 and rIBYZ strains. Furthermore, rH120-S1/YZ was found to provide effective protection against challenge with the QX-like nephropathogenic strain in young chickens. The level of antibody production in the serum of the vaccinated chickens detected by ELISA continued to increase after 7 dpi. Thus, rH120-S1/YZ may be considered a potential live vaccine candidate for protection against Chinese QX-like nephropathogenic IBV infection."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T44","span":{"begin":993,"end":1002},"obj":"Disease"}],"attributes":[{"id":"A44","pred":"mondo_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"}],"text":"In conclusion, we constructed the recombinant strain, rH120-S1/YZ, which was based on the backbone of the H120 vaccine strain, and replaced the S1 gene with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated from China using the reverse genetic system. The safety results showed that this recombinant strain was not lethal to one-day-old chicks and it had not gained the ability to tissue invasion or replication in the kidneys and lungs of infected animals. Moreover, the viral load of the chickens was significantly decreased compared with the rH120 and rIBYZ strains. Furthermore, rH120-S1/YZ was found to provide effective protection against challenge with the QX-like nephropathogenic strain in young chickens. The level of antibody production in the serum of the vaccinated chickens detected by ELISA continued to increase after 7 dpi. Thus, rH120-S1/YZ may be considered a potential live vaccine candidate for protection against Chinese QX-like nephropathogenic IBV infection."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T616","span":{"begin":60,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T617","span":{"begin":144,"end":146},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T618","span":{"begin":147,"end":151},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T619","span":{"begin":440,"end":447},"obj":"http://purl.obolibrary.org/obo/UBERON_0002113"},{"id":"T620","span":{"begin":440,"end":447},"obj":"http://www.ebi.ac.uk/efo/EFO_0000927"},{"id":"T621","span":{"begin":440,"end":447},"obj":"http://www.ebi.ac.uk/efo/EFO_0000929"},{"id":"T622","span":{"begin":452,"end":457},"obj":"http://www.ebi.ac.uk/efo/EFO_0000934"},{"id":"T623","span":{"begin":470,"end":477},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_33208"},{"id":"T624","span":{"begin":511,"end":519},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T625","span":{"begin":610,"end":612},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T626","span":{"begin":726,"end":734},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T627","span":{"begin":800,"end":808},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T628","span":{"begin":874,"end":876},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T629","span":{"begin":898,"end":899},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"In conclusion, we constructed the recombinant strain, rH120-S1/YZ, which was based on the backbone of the H120 vaccine strain, and replaced the S1 gene with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated from China using the reverse genetic system. The safety results showed that this recombinant strain was not lethal to one-day-old chicks and it had not gained the ability to tissue invasion or replication in the kidneys and lungs of infected animals. Moreover, the viral load of the chickens was significantly decreased compared with the rH120 and rIBYZ strains. Furthermore, rH120-S1/YZ was found to provide effective protection against challenge with the QX-like nephropathogenic strain in young chickens. The level of antibody production in the serum of the vaccinated chickens detected by ELISA continued to increase after 7 dpi. Thus, rH120-S1/YZ may be considered a potential live vaccine candidate for protection against Chinese QX-like nephropathogenic IBV infection."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T89","span":{"begin":749,"end":768},"obj":"http://purl.obolibrary.org/obo/GO_0002377"}],"text":"In conclusion, we constructed the recombinant strain, rH120-S1/YZ, which was based on the backbone of the H120 vaccine strain, and replaced the S1 gene with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated from China using the reverse genetic system. The safety results showed that this recombinant strain was not lethal to one-day-old chicks and it had not gained the ability to tissue invasion or replication in the kidneys and lungs of infected animals. Moreover, the viral load of the chickens was significantly decreased compared with the rH120 and rIBYZ strains. Furthermore, rH120-S1/YZ was found to provide effective protection against challenge with the QX-like nephropathogenic strain in young chickens. The level of antibody production in the serum of the vaccinated chickens detected by ELISA continued to increase after 7 dpi. Thus, rH120-S1/YZ may be considered a potential live vaccine candidate for protection against Chinese QX-like nephropathogenic IBV infection."}

{"project":"LitCovid-sentences","denotations":[{"id":"T267","span":{"begin":0,"end":272},"obj":"Sentence"},{"id":"T268","span":{"begin":273,"end":478},"obj":"Sentence"},{"id":"T269","span":{"begin":479,"end":590},"obj":"Sentence"},{"id":"T270","span":{"begin":591,"end":735},"obj":"Sentence"},{"id":"T271","span":{"begin":736,"end":861},"obj":"Sentence"},{"id":"T272","span":{"begin":862,"end":1003},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In conclusion, we constructed the recombinant strain, rH120-S1/YZ, which was based on the backbone of the H120 vaccine strain, and replaced the S1 gene with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated from China using the reverse genetic system. The safety results showed that this recombinant strain was not lethal to one-day-old chicks and it had not gained the ability to tissue invasion or replication in the kidneys and lungs of infected animals. Moreover, the viral load of the chickens was significantly decreased compared with the rH120 and rIBYZ strains. Furthermore, rH120-S1/YZ was found to provide effective protection against challenge with the QX-like nephropathogenic strain in young chickens. The level of antibody production in the serum of the vaccinated chickens detected by ELISA continued to increase after 7 dpi. Thus, rH120-S1/YZ may be considered a potential live vaccine candidate for protection against Chinese QX-like nephropathogenic IBV infection."}

{"project":"LitCovid-PubTator","denotations":[{"id":"606","span":{"begin":511,"end":519},"obj":"Species"},{"id":"607","span":{"begin":726,"end":734},"obj":"Species"},{"id":"608","span":{"begin":800,"end":808},"obj":"Species"},{"id":"609","span":{"begin":604,"end":609},"obj":"Chemical"},{"id":"610","span":{"begin":177,"end":193},"obj":"Disease"},{"id":"611","span":{"begin":461,"end":469},"obj":"Disease"},{"id":"612","span":{"begin":693,"end":709},"obj":"Disease"},{"id":"613","span":{"begin":972,"end":1002},"obj":"Disease"}],"attributes":[{"id":"A606","pred":"tao:has_database_id","subj":"606","obj":"Tax:9031"},{"id":"A607","pred":"tao:has_database_id","subj":"607","obj":"Tax:9031"},{"id":"A608","pred":"tao:has_database_id","subj":"608","obj":"Tax:9031"},{"id":"A611","pred":"tao:has_database_id","subj":"611","obj":"MESH:D007239"},{"id":"A613","pred":"tao:has_database_id","subj":"613","obj":"MESH:D007239"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"In conclusion, we constructed the recombinant strain, rH120-S1/YZ, which was based on the backbone of the H120 vaccine strain, and replaced the S1 gene with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated from China using the reverse genetic system. The safety results showed that this recombinant strain was not lethal to one-day-old chicks and it had not gained the ability to tissue invasion or replication in the kidneys and lungs of infected animals. Moreover, the viral load of the chickens was significantly decreased compared with the rH120 and rIBYZ strains. Furthermore, rH120-S1/YZ was found to provide effective protection against challenge with the QX-like nephropathogenic strain in young chickens. The level of antibody production in the serum of the vaccinated chickens detected by ELISA continued to increase after 7 dpi. Thus, rH120-S1/YZ may be considered a potential live vaccine candidate for protection against Chinese QX-like nephropathogenic IBV infection."}