PMC:7115396 / 19731-20735 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7115396","sourcedb":"PMC","sourceid":"7115396","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7115396","text":"The biological characteristics of the rIBVs in SPF chicken embryos. (A) Pathogenicity of recombinant IBVs in infected chick embryos. Five 10-old-day embryonated SPF chicken eggs per group were inoculated with 0.2 mL virus diluent of rH120, rIBYZ, or rH120-S1/YZ. Chick embryo lesions were observed at 144 h post-inoculation at 37 °C. (B) EID50 of the recombinant IBVs, rH120, rIBYZ, and rH120-S1/YZ in SPF chicken embryonated eggs. (C) The multicycle growth kinetics of the recombinant IBVs, rH120, rIBYZ, and rH120-S1/YZ in SPF chicken embryonated eggs. All data are presented as the mean ± standard deviation (SD). Some of the error bars are too small to be seen. The qRT-PCR was carried out with three replicates. Markers of statistical difference were acquired by comparing between rH120 group and rH120-S1/YZ group. ***Indicates an extremely significant difference at P ≤ 0.001; **Indicates a highly significant difference at 0.001 \u003c P ≤ 0.01; *Indicates a significant difference at 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