PMC:7115396 / 17684-18685 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T83","span":{"begin":431,"end":436},"obj":"Body_part"},{"id":"T84","span":{"begin":660,"end":663},"obj":"Body_part"},{"id":"T85","span":{"begin":700,"end":703},"obj":"Body_part"}],"attributes":[{"id":"A83","pred":"fma_id","subj":"T83","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A84","pred":"fma_id","subj":"T84","obj":"http://purl.org/sig/ont/fma/fma62872"},{"id":"A85","pred":"fma_id","subj":"T85","obj":"http://purl.org/sig/ont/fma/fma62872"}],"text":"45 1-day-old SPF chickens were divided into three groups (n = 15 per group). After vaccinating with the rH120 and rH120-S1/YZ strains at a dose of 104 EID50/200 μL, the sera of 10 birds from each group were randomly collected and antibodies were tested using an ELISA created in our lab at 7, 14, 21, and 28 days post-vaccination (dpv). ELISA plates were coated with antigen of culture supernatants of inactivated IBV rMJ , a vero cells adaption strain domesticated from its parental strain of IBYZ in our lab. Individual chicken sera collected from different groups diluted to 1:200 were loaded and the plates were incubated at 37 °C for 60 min. IBV-specific IgG was detected with anti-chicken IgY (IgG) (whole molecule)-peroxidase antibodies produced in rabbits (Sigma-Aldrich, Germany). After incubated with 3,3′,5,5′-Tetramethylbenzidine(TMB) substrate for 15 min and terminated with 2 mol/L H2SO4 solution, the absorbance at 490 nm was measured using a Model 680 microplate reader (Bio-Rad, USA)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T232","span":{"begin":0,"end":2},"obj":"http://purl.obolibrary.org/obo/CLO_0053799"},{"id":"T233","span":{"begin":17,"end":25},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T234","span":{"begin":120,"end":122},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T235","span":{"begin":137,"end":138},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T236","span":{"begin":246,"end":252},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T237","span":{"begin":283,"end":291},"obj":"http://purl.obolibrary.org/obo/PR_000001438"},{"id":"T238","span":{"begin":424,"end":425},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T239","span":{"begin":426,"end":430},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T240","span":{"begin":426,"end":430},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T241","span":{"begin":431,"end":436},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T242","span":{"begin":522,"end":529},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T243","span":{"begin":687,"end":694},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T244","span":{"begin":956,"end":957},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"45 1-day-old SPF chickens were divided into three groups (n = 15 per group). After vaccinating with the rH120 and rH120-S1/YZ strains at a dose of 104 EID50/200 μL, the sera of 10 birds from each group were randomly collected and antibodies were tested using an ELISA created in our lab at 7, 14, 21, and 28 days post-vaccination (dpv). ELISA plates were coated with antigen of culture supernatants of inactivated IBV rMJ , a vero cells adaption strain domesticated from its parental strain of IBYZ in our lab. Individual chicken sera collected from different groups diluted to 1:200 were loaded and the plates were incubated at 37 °C for 60 min. IBV-specific IgG was detected with anti-chicken IgY (IgG) (whole molecule)-peroxidase antibodies produced in rabbits (Sigma-Aldrich, Germany). After incubated with 3,3′,5,5′-Tetramethylbenzidine(TMB) substrate for 15 min and terminated with 2 mol/L H2SO4 solution, the absorbance at 490 nm was measured using a Model 680 microplate reader (Bio-Rad, USA)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T48","span":{"begin":13,"end":16},"obj":"Chemical"},{"id":"T49","span":{"begin":69,"end":74},"obj":"Chemical"},{"id":"T50","span":{"begin":196,"end":201},"obj":"Chemical"},{"id":"T51","span":{"begin":367,"end":374},"obj":"Chemical"},{"id":"T52","span":{"begin":712,"end":720},"obj":"Chemical"},{"id":"T53","span":{"begin":896,"end":901},"obj":"Chemical"},{"id":"T54","span":{"begin":902,"end":910},"obj":"Chemical"}],"attributes":[{"id":"A48","pred":"chebi_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/CHEBI_88542"},{"id":"A49","pred":"chebi_id","subj":"T49","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A50","pred":"chebi_id","subj":"T50","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A51","pred":"chebi_id","subj":"T51","obj":"http://purl.obolibrary.org/obo/CHEBI_59132"},{"id":"A52","pred":"chebi_id","subj":"T52","obj":"http://purl.obolibrary.org/obo/CHEBI_25367"},{"id":"A53","pred":"chebi_id","subj":"T53","obj":"http://purl.obolibrary.org/obo/CHEBI_26836"},{"id":"A54","pred":"chebi_id","subj":"T54","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"}],"text":"45 1-day-old SPF chickens were divided into three groups (n = 15 per group). After vaccinating with the rH120 and rH120-S1/YZ strains at a dose of 104 EID50/200 μL, the sera of 10 birds from each group were randomly collected and antibodies were tested using an ELISA created in our lab at 7, 14, 21, and 28 days post-vaccination (dpv). ELISA plates were coated with antigen of culture supernatants of inactivated IBV rMJ , a vero cells adaption strain domesticated from its parental strain of IBYZ in our lab. Individual chicken sera collected from different groups diluted to 1:200 were loaded and the plates were incubated at 37 °C for 60 min. IBV-specific IgG was detected with anti-chicken IgY (IgG) (whole molecule)-peroxidase antibodies produced in rabbits (Sigma-Aldrich, Germany). After incubated with 3,3′,5,5′-Tetramethylbenzidine(TMB) substrate for 15 min and terminated with 2 mol/L H2SO4 solution, the absorbance at 490 nm was measured using a Model 680 microplate reader (Bio-Rad, USA)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T123","span":{"begin":0,"end":76},"obj":"Sentence"},{"id":"T124","span":{"begin":77,"end":336},"obj":"Sentence"},{"id":"T125","span":{"begin":337,"end":510},"obj":"Sentence"},{"id":"T126","span":{"begin":511,"end":646},"obj":"Sentence"},{"id":"T127","span":{"begin":647,"end":789},"obj":"Sentence"},{"id":"T128","span":{"begin":790,"end":1001},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"45 1-day-old SPF chickens were divided into three groups (n = 15 per group). After vaccinating with the rH120 and rH120-S1/YZ strains at a dose of 104 EID50/200 μL, the sera of 10 birds from each group were randomly collected and antibodies were tested using an ELISA created in our lab at 7, 14, 21, and 28 days post-vaccination (dpv). ELISA plates were coated with antigen of culture supernatants of inactivated IBV rMJ , a vero cells adaption strain domesticated from its parental strain of IBYZ in our lab. Individual chicken sera collected from different groups diluted to 1:200 were loaded and the plates were incubated at 37 °C for 60 min. IBV-specific IgG was detected with anti-chicken IgY (IgG) (whole molecule)-peroxidase antibodies produced in rabbits (Sigma-Aldrich, Germany). After incubated with 3,3′,5,5′-Tetramethylbenzidine(TMB) substrate for 15 min and terminated with 2 mol/L H2SO4 solution, the absorbance at 490 nm was measured using a Model 680 microplate reader (Bio-Rad, USA)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"252","span":{"begin":17,"end":25},"obj":"Species"},{"id":"253","span":{"begin":522,"end":529},"obj":"Species"},{"id":"254","span":{"begin":756,"end":763},"obj":"Species"},{"id":"255","span":{"begin":414,"end":417},"obj":"Species"},{"id":"256","span":{"begin":647,"end":650},"obj":"Species"},{"id":"257","span":{"begin":687,"end":694},"obj":"Species"},{"id":"258","span":{"begin":494,"end":498},"obj":"Chemical"},{"id":"259","span":{"begin":811,"end":841},"obj":"Chemical"},{"id":"260","span":{"begin":842,"end":845},"obj":"Chemical"},{"id":"261","span":{"begin":896,"end":901},"obj":"Chemical"}],"attributes":[{"id":"A252","pred":"tao:has_database_id","subj":"252","obj":"Tax:9031"},{"id":"A253","pred":"tao:has_database_id","subj":"253","obj":"Tax:9031"},{"id":"A254","pred":"tao:has_database_id","subj":"254","obj":"Tax:9986"},{"id":"A255","pred":"tao:has_database_id","subj":"255","obj":"Tax:11120"},{"id":"A256","pred":"tao:has_database_id","subj":"256","obj":"Tax:11120"},{"id":"A257","pred":"tao:has_database_id","subj":"257","obj":"Tax:9031"},{"id":"A259","pred":"tao:has_database_id","subj":"259","obj":"MESH:C021758"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"45 1-day-old SPF chickens were divided into three groups (n = 15 per group). After vaccinating with the rH120 and rH120-S1/YZ strains at a dose of 104 EID50/200 μL, the sera of 10 birds from each group were randomly collected and antibodies were tested using an ELISA created in our lab at 7, 14, 21, and 28 days post-vaccination (dpv). ELISA plates were coated with antigen of culture supernatants of inactivated IBV rMJ , a vero cells adaption strain domesticated from its parental strain of IBYZ in our lab. Individual chicken sera collected from different groups diluted to 1:200 were loaded and the plates were incubated at 37 °C for 60 min. IBV-specific IgG was detected with anti-chicken IgY (IgG) (whole molecule)-peroxidase antibodies produced in rabbits (Sigma-Aldrich, Germany). After incubated with 3,3′,5,5′-Tetramethylbenzidine(TMB) substrate for 15 min and terminated with 2 mol/L H2SO4 solution, the absorbance at 490 nm was measured using a Model 680 microplate reader (Bio-Rad, USA)."}