PMC:7100515 / 44216-44819 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T394","span":{"begin":37,"end":42},"obj":"Body_part"},{"id":"T395","span":{"begin":123,"end":130},"obj":"Body_part"},{"id":"T396","span":{"begin":132,"end":137},"obj":"Body_part"},{"id":"T397","span":{"begin":331,"end":336},"obj":"Body_part"},{"id":"T398","span":{"begin":389,"end":397},"obj":"Body_part"},{"id":"T399","span":{"begin":493,"end":501},"obj":"Body_part"},{"id":"T400","span":{"begin":560,"end":565},"obj":"Body_part"}],"attributes":[{"id":"A394","pred":"fma_id","subj":"T394","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A395","pred":"fma_id","subj":"T395","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A396","pred":"fma_id","subj":"T396","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A397","pred":"fma_id","subj":"T397","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A398","pred":"fma_id","subj":"T398","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A399","pred":"fma_id","subj":"T399","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A400","pred":"fma_id","subj":"T400","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Soluble hACE2-binding assay\nHEK-293T cells were transfected with plasmids encoding SARS-CoV-2 S, SARS-CoV S, or MERS-CoV S protein. Cells were detached with 1 mM EDTA 40 h post transfection, washed twice with 3% FBS in 1×PBS, and incubated with 5 μg/ml soluble hACE2 for 1 h on ice. After washing three times with 3% FBS in 1×PBS, cells were incubated with polyclonal goat anti-human ACE2 antibody (1:200) (R\u0026D Systems, MN, USA) for 1 h, followed by FITC-conjugated rabbit anti-goat secondary antibody (1:500) (Jackson ImmunoResearch, PA, USA). After washing, cells were then analyzed by flow cytometry."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T367","span":{"begin":83,"end":91},"obj":"Disease"},{"id":"T368","span":{"begin":97,"end":105},"obj":"Disease"}],"attributes":[{"id":"A367","pred":"mondo_id","subj":"T367","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A368","pred":"mondo_id","subj":"T368","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Soluble hACE2-binding assay\nHEK-293T cells were transfected with plasmids encoding SARS-CoV-2 S, SARS-CoV S, or MERS-CoV S protein. Cells were detached with 1 mM EDTA 40 h post transfection, washed twice with 3% FBS in 1×PBS, and incubated with 5 μg/ml soluble hACE2 for 1 h on ice. After washing three times with 3% FBS in 1×PBS, cells were incubated with polyclonal goat anti-human ACE2 antibody (1:200) (R\u0026D Systems, MN, USA) for 1 h, followed by FITC-conjugated rabbit anti-goat secondary antibody (1:500) (Jackson ImmunoResearch, PA, USA). After washing, cells were then analyzed by flow cytometry."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T978","span":{"begin":32,"end":36},"obj":"http://purl.obolibrary.org/obo/CLO_0050894"},{"id":"T979","span":{"begin":32,"end":36},"obj":"http://purl.obolibrary.org/obo/CLO_0051650"},{"id":"T980","span":{"begin":32,"end":36},"obj":"http://purl.obolibrary.org/obo/CLO_0052052"},{"id":"T981","span":{"begin":37,"end":42},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T982","span":{"begin":132,"end":137},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T983","span":{"begin":331,"end":336},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T984","span":{"begin":368,"end":372},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T985","span":{"begin":378,"end":383},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T986","span":{"begin":407,"end":410},"obj":"http://purl.obolibrary.org/obo/CLO_0008693"},{"id":"T987","span":{"begin":407,"end":410},"obj":"http://purl.obolibrary.org/obo/CLO_0008770"},{"id":"T988","span":{"begin":478,"end":482},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T989","span":{"begin":560,"end":565},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Soluble hACE2-binding assay\nHEK-293T cells were transfected with plasmids encoding SARS-CoV-2 S, SARS-CoV S, or MERS-CoV S protein. Cells were detached with 1 mM EDTA 40 h post transfection, washed twice with 3% FBS in 1×PBS, and incubated with 5 μg/ml soluble hACE2 for 1 h on ice. After washing three times with 3% FBS in 1×PBS, cells were incubated with polyclonal goat anti-human ACE2 antibody (1:200) (R\u0026D Systems, MN, USA) for 1 h, followed by FITC-conjugated rabbit anti-goat secondary antibody (1:500) (Jackson ImmunoResearch, PA, USA). After washing, cells were then analyzed by flow cytometry."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T391","span":{"begin":123,"end":130},"obj":"Chemical"},{"id":"T392","span":{"begin":162,"end":166},"obj":"Chemical"},{"id":"T394","span":{"begin":420,"end":422},"obj":"Chemical"},{"id":"T395","span":{"begin":450,"end":454},"obj":"Chemical"},{"id":"T396","span":{"begin":535,"end":537},"obj":"Chemical"}],"attributes":[{"id":"A391","pred":"chebi_id","subj":"T391","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A392","pred":"chebi_id","subj":"T392","obj":"http://purl.obolibrary.org/obo/CHEBI_42191"},{"id":"A393","pred":"chebi_id","subj":"T392","obj":"http://purl.obolibrary.org/obo/CHEBI_64755"},{"id":"A394","pred":"chebi_id","subj":"T394","obj":"http://purl.obolibrary.org/obo/CHEBI_141442"},{"id":"A395","pred":"chebi_id","subj":"T395","obj":"http://purl.obolibrary.org/obo/CHEBI_37926"},{"id":"A396","pred":"chebi_id","subj":"T396","obj":"http://purl.obolibrary.org/obo/CHEBI_30745"},{"id":"A397","pred":"chebi_id","subj":"T396","obj":"http://purl.obolibrary.org/obo/CHEBI_30768"},{"id":"A398","pred":"chebi_id","subj":"T396","obj":"http://purl.obolibrary.org/obo/CHEBI_74753"},{"id":"A399","pred":"chebi_id","subj":"T396","obj":"http://purl.obolibrary.org/obo/CHEBI_7959"},{"id":"A400","pred":"chebi_id","subj":"T396","obj":"http://purl.obolibrary.org/obo/CHEBI_8429"}],"text":"Soluble hACE2-binding assay\nHEK-293T cells were transfected with plasmids encoding SARS-CoV-2 S, SARS-CoV S, or MERS-CoV S protein. Cells were detached with 1 mM EDTA 40 h post transfection, washed twice with 3% FBS in 1×PBS, and incubated with 5 μg/ml soluble hACE2 for 1 h on ice. After washing three times with 3% FBS in 1×PBS, cells were incubated with polyclonal goat anti-human ACE2 antibody (1:200) (R\u0026D Systems, MN, USA) for 1 h, followed by FITC-conjugated rabbit anti-goat secondary antibody (1:500) (Jackson ImmunoResearch, PA, USA). After washing, cells were then analyzed by flow cytometry."}

{"project":"LitCovid-sentences","denotations":[{"id":"T318","span":{"begin":0,"end":27},"obj":"Sentence"},{"id":"T319","span":{"begin":28,"end":131},"obj":"Sentence"},{"id":"T320","span":{"begin":132,"end":282},"obj":"Sentence"},{"id":"T321","span":{"begin":283,"end":544},"obj":"Sentence"},{"id":"T322","span":{"begin":545,"end":603},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Soluble hACE2-binding assay\nHEK-293T cells were transfected with plasmids encoding SARS-CoV-2 S, SARS-CoV S, or MERS-CoV S protein. Cells were detached with 1 mM EDTA 40 h post transfection, washed twice with 3% FBS in 1×PBS, and incubated with 5 μg/ml soluble hACE2 for 1 h on ice. After washing three times with 3% FBS in 1×PBS, cells were incubated with polyclonal goat anti-human ACE2 antibody (1:200) (R\u0026D Systems, MN, USA) for 1 h, followed by FITC-conjugated rabbit anti-goat secondary antibody (1:500) (Jackson ImmunoResearch, PA, USA). After washing, cells were then analyzed by flow cytometry."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1705","span":{"begin":8,"end":13},"obj":"Gene"},{"id":"1717","span":{"begin":261,"end":266},"obj":"Gene"},{"id":"1718","span":{"begin":384,"end":388},"obj":"Gene"},{"id":"1719","span":{"begin":94,"end":95},"obj":"Gene"},{"id":"1720","span":{"begin":83,"end":93},"obj":"Species"},{"id":"1721","span":{"begin":97,"end":105},"obj":"Species"},{"id":"1722","span":{"begin":112,"end":120},"obj":"Species"},{"id":"1723","span":{"begin":378,"end":383},"obj":"Species"},{"id":"1724","span":{"begin":162,"end":166},"obj":"Chemical"},{"id":"1725","span":{"begin":450,"end":454},"obj":"Chemical"},{"id":"1726","span":{"begin":28,"end":36},"obj":"CellLine"},{"id":"1727","span":{"begin":420,"end":422},"obj":"CellLine"}],"attributes":[{"id":"A1705","pred":"tao:has_database_id","subj":"1705","obj":"Gene:59272"},{"id":"A1717","pred":"tao:has_database_id","subj":"1717","obj":"Gene:59272"},{"id":"A1718","pred":"tao:has_database_id","subj":"1718","obj":"Gene:59272"},{"id":"A1719","pred":"tao:has_database_id","subj":"1719","obj":"Gene:43740568"},{"id":"A1720","pred":"tao:has_database_id","subj":"1720","obj":"Tax:2697049"},{"id":"A1721","pred":"tao:has_database_id","subj":"1721","obj":"Tax:694009"},{"id":"A1722","pred":"tao:has_database_id","subj":"1722","obj":"Tax:1335626"},{"id":"A1723","pred":"tao:has_database_id","subj":"1723","obj":"Tax:9606"},{"id":"A1724","pred":"tao:has_database_id","subj":"1724","obj":"MESH:D004492"},{"id":"A1725","pred":"tao:has_database_id","subj":"1725","obj":"MESH:D016650"},{"id":"A1726","pred":"tao:has_database_id","subj":"1726","obj":"CVCL:0063"},{"id":"A1727","pred":"tao:has_database_id","subj":"1727","obj":"CVCL:U508"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Soluble hACE2-binding assay\nHEK-293T cells were transfected with plasmids encoding SARS-CoV-2 S, SARS-CoV S, or MERS-CoV S protein. Cells were detached with 1 mM EDTA 40 h post transfection, washed twice with 3% FBS in 1×PBS, and incubated with 5 μg/ml soluble hACE2 for 1 h on ice. After washing three times with 3% FBS in 1×PBS, cells were incubated with polyclonal goat anti-human ACE2 antibody (1:200) (R\u0026D Systems, MN, USA) for 1 h, followed by FITC-conjugated rabbit anti-goat secondary antibody (1:500) (Jackson ImmunoResearch, PA, USA). After washing, cells were then analyzed by flow cytometry."}