PMC:7100515 / 38801-39718 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T344","span":{"begin":52,"end":57},"obj":"Body_part"},{"id":"T345","span":{"begin":333,"end":337},"obj":"Body_part"},{"id":"T346","span":{"begin":359,"end":364},"obj":"Body_part"},{"id":"T347","span":{"begin":385,"end":390},"obj":"Body_part"},{"id":"T348","span":{"begin":510,"end":515},"obj":"Body_part"},{"id":"T349","span":{"begin":572,"end":577},"obj":"Body_part"}],"attributes":[{"id":"A344","pred":"fma_id","subj":"T344","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A345","pred":"fma_id","subj":"T345","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A346","pred":"fma_id","subj":"T346","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A347","pred":"fma_id","subj":"T347","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A348","pred":"fma_id","subj":"T348","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A349","pred":"fma_id","subj":"T349","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Pseudovirions were produced by co-transfection 293T cells with psPAX2, pLenti-GFP, and plasmids encoding either SARS-CoV-2 S, SARS-CoV S, VSV-G, or empty vector by using polyetherimide (PEI). The supernatants were harvested at 40, 64 h post transfection, passed through 0.45 μm filter, and centrifuged at 800 × g for 5 min to remove cell debris. To transduce cells with pseudovirions, cells were seeded into 24-well plates and inoculated with 500 μl media containing pseudovirions. After overnight incubation, cells were fed with fresh media. About 40 h post inoculation, cells were lysed with 120 μl medium containing 50% Steady-glo (promega) at room temperature for 5 min. The transduction efficiency was measured by quantification of the luciferase activity using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, CA, USA). All experiments were done in triplicates, and repeated at least twice or more."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T348","span":{"begin":112,"end":120},"obj":"Disease"},{"id":"T349","span":{"begin":126,"end":134},"obj":"Disease"}],"attributes":[{"id":"A348","pred":"mondo_id","subj":"T348","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A349","pred":"mondo_id","subj":"T349","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Pseudovirions were produced by co-transfection 293T cells with psPAX2, pLenti-GFP, and plasmids encoding either SARS-CoV-2 S, SARS-CoV S, VSV-G, or empty vector by using polyetherimide (PEI). The supernatants were harvested at 40, 64 h post transfection, passed through 0.45 μm filter, and centrifuged at 800 × g for 5 min to remove cell debris. To transduce cells with pseudovirions, cells were seeded into 24-well plates and inoculated with 500 μl media containing pseudovirions. After overnight incubation, cells were fed with fresh media. About 40 h post inoculation, cells were lysed with 120 μl medium containing 50% Steady-glo (promega) at room temperature for 5 min. The transduction efficiency was measured by quantification of the luciferase activity using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, CA, USA). All experiments were done in triplicates, and repeated at least twice or more."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T877","span":{"begin":47,"end":51},"obj":"http://purl.obolibrary.org/obo/CLO_0050894"},{"id":"T878","span":{"begin":47,"end":51},"obj":"http://purl.obolibrary.org/obo/CLO_0051650"},{"id":"T879","span":{"begin":47,"end":51},"obj":"http://purl.obolibrary.org/obo/CLO_0052052"},{"id":"T880","span":{"begin":52,"end":57},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T881","span":{"begin":78,"end":81},"obj":"http://purl.obolibrary.org/obo/CLO_0053478"},{"id":"T882","span":{"begin":333,"end":337},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T883","span":{"begin":359,"end":364},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T884","span":{"begin":385,"end":390},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T885","span":{"begin":510,"end":515},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T886","span":{"begin":572,"end":577},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T887","span":{"begin":752,"end":760},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T888","span":{"begin":767,"end":768},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Pseudovirions were produced by co-transfection 293T cells with psPAX2, pLenti-GFP, and plasmids encoding either SARS-CoV-2 S, SARS-CoV S, VSV-G, or empty vector by using polyetherimide (PEI). The supernatants were harvested at 40, 64 h post transfection, passed through 0.45 μm filter, and centrifuged at 800 × g for 5 min to remove cell debris. To transduce cells with pseudovirions, cells were seeded into 24-well plates and inoculated with 500 μl media containing pseudovirions. After overnight incubation, cells were fed with fresh media. About 40 h post inoculation, cells were lysed with 120 μl medium containing 50% Steady-glo (promega) at room temperature for 5 min. The transduction efficiency was measured by quantification of the luciferase activity using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, CA, USA). All experiments were done in triplicates, and repeated at least twice or more."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T330","span":{"begin":186,"end":189},"obj":"Chemical"},{"id":"T332","span":{"begin":777,"end":779},"obj":"Chemical"}],"attributes":[{"id":"A330","pred":"chebi_id","subj":"T330","obj":"http://purl.obolibrary.org/obo/CHEBI_144613"},{"id":"A331","pred":"chebi_id","subj":"T330","obj":"http://purl.obolibrary.org/obo/CHEBI_53231"},{"id":"A332","pred":"chebi_id","subj":"T332","obj":"http://purl.obolibrary.org/obo/CHEBI_74067"}],"text":"Pseudovirions were produced by co-transfection 293T cells with psPAX2, pLenti-GFP, and plasmids encoding either SARS-CoV-2 S, SARS-CoV S, VSV-G, or empty vector by using polyetherimide (PEI). The supernatants were harvested at 40, 64 h post transfection, passed through 0.45 μm filter, and centrifuged at 800 × g for 5 min to remove cell debris. To transduce cells with pseudovirions, cells were seeded into 24-well plates and inoculated with 500 μl media containing pseudovirions. After overnight incubation, cells were fed with fresh media. About 40 h post inoculation, cells were lysed with 120 μl medium containing 50% Steady-glo (promega) at room temperature for 5 min. The transduction efficiency was measured by quantification of the luciferase activity using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, CA, USA). All experiments were done in triplicates, and repeated at least twice or more."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T78","span":{"begin":679,"end":691},"obj":"http://purl.obolibrary.org/obo/GO_0009293"}],"text":"Pseudovirions were produced by co-transfection 293T cells with psPAX2, pLenti-GFP, and plasmids encoding either SARS-CoV-2 S, SARS-CoV S, VSV-G, or empty vector by using polyetherimide (PEI). The supernatants were harvested at 40, 64 h post transfection, passed through 0.45 μm filter, and centrifuged at 800 × g for 5 min to remove cell debris. To transduce cells with pseudovirions, cells were seeded into 24-well plates and inoculated with 500 μl media containing pseudovirions. After overnight incubation, cells were fed with fresh media. About 40 h post inoculation, cells were lysed with 120 μl medium containing 50% Steady-glo (promega) at room temperature for 5 min. The transduction efficiency was measured by quantification of the luciferase activity using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, CA, USA). All experiments were done in triplicates, and repeated at least twice or more."}

{"project":"LitCovid-sentences","denotations":[{"id":"T279","span":{"begin":0,"end":191},"obj":"Sentence"},{"id":"T280","span":{"begin":192,"end":345},"obj":"Sentence"},{"id":"T281","span":{"begin":346,"end":481},"obj":"Sentence"},{"id":"T282","span":{"begin":482,"end":542},"obj":"Sentence"},{"id":"T283","span":{"begin":543,"end":674},"obj":"Sentence"},{"id":"T284","span":{"begin":675,"end":838},"obj":"Sentence"},{"id":"T285","span":{"begin":839,"end":917},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Pseudovirions were produced by co-transfection 293T cells with psPAX2, pLenti-GFP, and plasmids encoding either SARS-CoV-2 S, SARS-CoV S, VSV-G, or empty vector by using polyetherimide (PEI). The supernatants were harvested at 40, 64 h post transfection, passed through 0.45 μm filter, and centrifuged at 800 × g for 5 min to remove cell debris. To transduce cells with pseudovirions, cells were seeded into 24-well plates and inoculated with 500 μl media containing pseudovirions. After overnight incubation, cells were fed with fresh media. About 40 h post inoculation, cells were lysed with 120 μl medium containing 50% Steady-glo (promega) at room temperature for 5 min. The transduction efficiency was measured by quantification of the luciferase activity using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, CA, USA). All experiments were done in triplicates, and repeated at least twice or more."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1596","span":{"begin":123,"end":124},"obj":"Gene"},{"id":"1597","span":{"begin":112,"end":122},"obj":"Species"},{"id":"1598","span":{"begin":126,"end":134},"obj":"Species"},{"id":"1599","span":{"begin":170,"end":184},"obj":"Chemical"},{"id":"1600","span":{"begin":186,"end":189},"obj":"Chemical"},{"id":"1601","span":{"begin":47,"end":51},"obj":"CellLine"}],"attributes":[{"id":"A1596","pred":"tao:has_database_id","subj":"1596","obj":"Gene:43740568"},{"id":"A1597","pred":"tao:has_database_id","subj":"1597","obj":"Tax:2697049"},{"id":"A1598","pred":"tao:has_database_id","subj":"1598","obj":"Tax:694009"},{"id":"A1599","pred":"tao:has_database_id","subj":"1599","obj":"MESH:C433673"},{"id":"A1600","pred":"tao:has_database_id","subj":"1600","obj":"MESH:C433673"},{"id":"A1601","pred":"tao:has_database_id","subj":"1601","obj":"CVCL:0063"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Pseudovirions were produced by co-transfection 293T cells with psPAX2, pLenti-GFP, and plasmids encoding either SARS-CoV-2 S, SARS-CoV S, VSV-G, or empty vector by using polyetherimide (PEI). The supernatants were harvested at 40, 64 h post transfection, passed through 0.45 μm filter, and centrifuged at 800 × g for 5 min to remove cell debris. To transduce cells with pseudovirions, cells were seeded into 24-well plates and inoculated with 500 μl media containing pseudovirions. After overnight incubation, cells were fed with fresh media. About 40 h post inoculation, cells were lysed with 120 μl medium containing 50% Steady-glo (promega) at room temperature for 5 min. The transduction efficiency was measured by quantification of the luciferase activity using a Modulus II microplate reader (Turner Biosystems, Sunnyvale, CA, USA). All experiments were done in triplicates, and repeated at least twice or more."}