PMC:7100515 / 35063-36105 JSON TXT

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LitCovid-PD-FMA-UBERON

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T289","span":{"begin":85,"end":97},"obj":"Body_part"},{"id":"T290","span":{"begin":130,"end":141},"obj":"Body_part"},{"id":"T291","span":{"begin":194,"end":198},"obj":"Body_part"},{"id":"T292","span":{"begin":299,"end":311},"obj":"Body_part"},{"id":"T293","span":{"begin":324,"end":336},"obj":"Body_part"},{"id":"T294","span":{"begin":347,"end":359},"obj":"Body_part"},{"id":"T295","span":{"begin":433,"end":436},"obj":"Body_part"},{"id":"T296","span":{"begin":482,"end":489},"obj":"Body_part"},{"id":"T297","span":{"begin":523,"end":534},"obj":"Body_part"},{"id":"T298","span":{"begin":536,"end":538},"obj":"Body_part"},{"id":"T299","span":{"begin":552,"end":559},"obj":"Body_part"},{"id":"T300","span":{"begin":593,"end":595},"obj":"Body_part"},{"id":"T301","span":{"begin":656,"end":663},"obj":"Body_part"}],"attributes":[{"id":"A289","pred":"fma_id","subj":"T289","obj":"http://purl.org/sig/ont/fma/fma62925"},{"id":"A290","pred":"fma_id","subj":"T290","obj":"http://purl.org/sig/ont/fma/fma82739"},{"id":"A291","pred":"fma_id","subj":"T291","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A292","pred":"fma_id","subj":"T292","obj":"http://purl.org/sig/ont/fma/fma62925"},{"id":"A293","pred":"fma_id","subj":"T293","obj":"http://purl.org/sig/ont/fma/fma62925"},{"id":"A294","pred":"fma_id","subj":"T294","obj":"http://purl.org/sig/ont/fma/fma62925"},{"id":"A295","pred":"fma_id","subj":"T295","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A296","pred":"fma_id","subj":"T296","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A297","pred":"fma_id","subj":"T297","obj":"http://purl.org/sig/ont/fma/fma82739"},{"id":"A298","pred":"fma_id","subj":"T298","obj":"http://purl.org/sig/ont/fma/fma82739"},{"id":"A299","pred":"fma_id","subj":"T299","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A300","pred":"fma_id","subj":"T300","obj":"http://purl.org/sig/ont/fma/fma82739"},{"id":"A301","pred":"fma_id","subj":"T301","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"Codon-optimized cDNA (sequence shown in Supplementary Table 2) encoding SARS-CoV-2 S glycoprotein (QHU36824.1) with C-terminal 19 amino acids deletion was synthesized and cloned into eukaryotic cell expression vector pCMV14-3×Flag between the Hind III and BamH I sites. Plasmids encoding SARS-CoV S glycoprotein, MERS-CoV S glycoprotein and MHV S glycoprotein (sequences shown in Supplementary Table 2) were constructed by inserting DNA fragment encoding codon-optimized SARS-CoV S protein (AAP13441.1) lacking the last 19 amino acids (aa), MERS-CoV S protein (AFS88936.1) lacking the last 16 aa but with a FLAG tag at the C terminus, or full-length MHV S protein (AAU06356.1) into pcDNA3.1between BamH I and Not I sites, respectively. The VSV-G encoding plasmid and lentiviral packaging plasmid psPAX2 were obtained from Addgene (Cambridge, MA). The pLenti-GFP lentiviral reporter plasmid that expresses GFP and luciferase was generously gifted by Fang Li, Duke University. All primers used in this study are listed in Supplementary Table 1."}

LitCovid-PD-MONDO

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T338","span":{"begin":72,"end":80},"obj":"Disease"},{"id":"T339","span":{"begin":288,"end":296},"obj":"Disease"},{"id":"T340","span":{"begin":471,"end":479},"obj":"Disease"}],"attributes":[{"id":"A338","pred":"mondo_id","subj":"T338","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A339","pred":"mondo_id","subj":"T339","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A340","pred":"mondo_id","subj":"T340","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Codon-optimized cDNA (sequence shown in Supplementary Table 2) encoding SARS-CoV-2 S glycoprotein (QHU36824.1) with C-terminal 19 amino acids deletion was synthesized and cloned into eukaryotic cell expression vector pCMV14-3×Flag between the Hind III and BamH I sites. Plasmids encoding SARS-CoV S glycoprotein, MERS-CoV S glycoprotein and MHV S glycoprotein (sequences shown in Supplementary Table 2) were constructed by inserting DNA fragment encoding codon-optimized SARS-CoV S protein (AAP13441.1) lacking the last 19 amino acids (aa), MERS-CoV S protein (AFS88936.1) lacking the last 16 aa but with a FLAG tag at the C terminus, or full-length MHV S protein (AAU06356.1) into pcDNA3.1between BamH I and Not I sites, respectively. The VSV-G encoding plasmid and lentiviral packaging plasmid psPAX2 were obtained from Addgene (Cambridge, MA). The pLenti-GFP lentiviral reporter plasmid that expresses GFP and luciferase was generously gifted by Fang Li, Duke University. All primers used in this study are listed in Supplementary Table 1."}

LitCovid-PD-CLO

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T752","span":{"begin":183,"end":193},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_2759"},{"id":"T753","span":{"begin":194,"end":198},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T754","span":{"begin":536,"end":538},"obj":"http://purl.obolibrary.org/obo/CLO_0001627"},{"id":"T755","span":{"begin":593,"end":595},"obj":"http://purl.obolibrary.org/obo/CLO_0001627"},{"id":"T756","span":{"begin":605,"end":606},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T757","span":{"begin":858,"end":861},"obj":"http://purl.obolibrary.org/obo/CLO_0053478"},{"id":"T758","span":{"begin":905,"end":908},"obj":"http://purl.obolibrary.org/obo/CLO_0053478"},{"id":"T759","span":{"begin":954,"end":956},"obj":"http://purl.obolibrary.org/obo/CLO_0001022"},{"id":"T760","span":{"begin":954,"end":956},"obj":"http://purl.obolibrary.org/obo/CLO_0007314"}],"text":"Codon-optimized cDNA (sequence shown in Supplementary Table 2) encoding SARS-CoV-2 S glycoprotein (QHU36824.1) with C-terminal 19 amino acids deletion was synthesized and cloned into eukaryotic cell expression vector pCMV14-3×Flag between the Hind III and BamH I sites. Plasmids encoding SARS-CoV S glycoprotein, MERS-CoV S glycoprotein and MHV S glycoprotein (sequences shown in Supplementary Table 2) were constructed by inserting DNA fragment encoding codon-optimized SARS-CoV S protein (AAP13441.1) lacking the last 19 amino acids (aa), MERS-CoV S protein (AFS88936.1) lacking the last 16 aa but with a FLAG tag at the C terminus, or full-length MHV S protein (AAU06356.1) into pcDNA3.1between BamH I and Not I sites, respectively. The VSV-G encoding plasmid and lentiviral packaging plasmid psPAX2 were obtained from Addgene (Cambridge, MA). The pLenti-GFP lentiviral reporter plasmid that expresses GFP and luciferase was generously gifted by Fang Li, Duke University. All primers used in this study are listed in Supplementary Table 1."}

LitCovid-PD-CHEBI

LitCovid-sentences

{"project":"LitCovid-sentences","denotations":[{"id":"T257","span":{"begin":0,"end":269},"obj":"Sentence"},{"id":"T258","span":{"begin":270,"end":735},"obj":"Sentence"},{"id":"T259","span":{"begin":736,"end":846},"obj":"Sentence"},{"id":"T260","span":{"begin":847,"end":974},"obj":"Sentence"},{"id":"T261","span":{"begin":975,"end":1042},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Codon-optimized cDNA (sequence shown in Supplementary Table 2) encoding SARS-CoV-2 S glycoprotein (QHU36824.1) with C-terminal 19 amino acids deletion was synthesized and cloned into eukaryotic cell expression vector pCMV14-3×Flag between the Hind III and BamH I sites. Plasmids encoding SARS-CoV S glycoprotein, MERS-CoV S glycoprotein and MHV S glycoprotein (sequences shown in Supplementary Table 2) were constructed by inserting DNA fragment encoding codon-optimized SARS-CoV S protein (AAP13441.1) lacking the last 19 amino acids (aa), MERS-CoV S protein (AFS88936.1) lacking the last 16 aa but with a FLAG tag at the C terminus, or full-length MHV S protein (AAU06356.1) into pcDNA3.1between BamH I and Not I sites, respectively. The VSV-G encoding plasmid and lentiviral packaging plasmid psPAX2 were obtained from Addgene (Cambridge, MA). The pLenti-GFP lentiviral reporter plasmid that expresses GFP and luciferase was generously gifted by Fang Li, Duke University. All primers used in this study are listed in Supplementary Table 1."}

LitCovid-PubTator

{"project":"LitCovid-PubTator","denotations":[{"id":"1460","span":{"begin":83,"end":84},"obj":"Gene"},{"id":"1461","span":{"begin":72,"end":82},"obj":"Species"},{"id":"1462","span":{"begin":288,"end":296},"obj":"Species"},{"id":"1463","span":{"begin":313,"end":321},"obj":"Species"},{"id":"1464","span":{"begin":341,"end":346},"obj":"Species"},{"id":"1465","span":{"begin":471,"end":479},"obj":"Species"},{"id":"1466","span":{"begin":541,"end":549},"obj":"Species"},{"id":"1467","span":{"begin":650,"end":655},"obj":"Species"}],"attributes":[{"id":"A1460","pred":"tao:has_database_id","subj":"1460","obj":"Gene:43740568"},{"id":"A1461","pred":"tao:has_database_id","subj":"1461","obj":"Tax:2697049"},{"id":"A1462","pred":"tao:has_database_id","subj":"1462","obj":"Tax:694009"},{"id":"A1463","pred":"tao:has_database_id","subj":"1463","obj":"Tax:1335626"},{"id":"A1464","pred":"tao:has_database_id","subj":"1464","obj":"Tax:11145"},{"id":"A1465","pred":"tao:has_database_id","subj":"1465","obj":"Tax:694009"},{"id":"A1466","pred":"tao:has_database_id","subj":"1466","obj":"Tax:1335626"},{"id":"A1467","pred":"tao:has_database_id","subj":"1467","obj":"Tax:11145"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Codon-optimized cDNA (sequence shown in Supplementary Table 2) encoding SARS-CoV-2 S glycoprotein (QHU36824.1) with C-terminal 19 amino acids deletion was synthesized and cloned into eukaryotic cell expression vector pCMV14-3×Flag between the Hind III and BamH I sites. Plasmids encoding SARS-CoV S glycoprotein, MERS-CoV S glycoprotein and MHV S glycoprotein (sequences shown in Supplementary Table 2) were constructed by inserting DNA fragment encoding codon-optimized SARS-CoV S protein (AAP13441.1) lacking the last 19 amino acids (aa), MERS-CoV S protein (AFS88936.1) lacking the last 16 aa but with a FLAG tag at the C terminus, or full-length MHV S protein (AAU06356.1) into pcDNA3.1between BamH I and Not I sites, respectively. The VSV-G encoding plasmid and lentiviral packaging plasmid psPAX2 were obtained from Addgene (Cambridge, MA). The pLenti-GFP lentiviral reporter plasmid that expresses GFP and luciferase was generously gifted by Fang Li, Duke University. All primers used in this study are listed in Supplementary Table 1."}