PMC:7094172 / 4823-5755 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T167","span":{"begin":39,"end":47},"obj":"SP_9"},{"id":"T166","span":{"begin":334,"end":343},"obj":"CHEBI:36357;CHEBI:36357"},{"id":"T165","span":{"begin":560,"end":573},"obj":"SO:0000984"}],"text":"We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID: 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1). Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B). The monomers shared a similar structural core preceded by a flexible region (Figure S1D). The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent. Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T33","span":{"begin":634,"end":646},"obj":"Body_part"},{"id":"T34","span":{"begin":807,"end":815},"obj":"Body_part"}],"attributes":[{"id":"A33","pred":"fma_id","subj":"T33","obj":"http://purl.org/sig/ont/fma/fma9713"},{"id":"A34","pred":"fma_id","subj":"T34","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID: 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1). Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B). The monomers shared a similar structural core preceded by a flexible region (Figure S1D). The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent. Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T21","span":{"begin":50,"end":53},"obj":"Disease"},{"id":"T23","span":{"begin":119,"end":122},"obj":"Disease"},{"id":"T25","span":{"begin":330,"end":333},"obj":"Disease"}],"attributes":[{"id":"A21","pred":"mondo_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A22","pred":"mondo_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"id":"A23","pred":"mondo_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A24","pred":"mondo_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"id":"A25","pred":"mondo_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A26","pred":"mondo_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"}],"text":"We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID: 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1). Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B). The monomers shared a similar structural core preceded by a flexible region (Figure S1D). The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent. Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T31","span":{"begin":80,"end":82},"obj":"http://purl.obolibrary.org/obo/CLO_0007860"},{"id":"T32","span":{"begin":258,"end":259},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T33","span":{"begin":278,"end":279},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T34","span":{"begin":287,"end":289},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T35","span":{"begin":409,"end":410},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T36","span":{"begin":442,"end":443},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T37","span":{"begin":466,"end":467},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T38","span":{"begin":504,"end":505},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T39","span":{"begin":558,"end":559},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T40","span":{"begin":632,"end":633},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T41","span":{"begin":869,"end":870},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID: 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1). Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B). The monomers shared a similar structural core preceded by a flexible region (Figure S1D). The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent. Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T53","span":{"begin":80,"end":82},"obj":"Chemical"},{"id":"T54","span":{"begin":128,"end":130},"obj":"Chemical"},{"id":"T55","span":{"begin":334,"end":343},"obj":"Chemical"},{"id":"T56","span":{"begin":807,"end":815},"obj":"Chemical"}],"attributes":[{"id":"A53","pred":"chebi_id","subj":"T53","obj":"http://purl.obolibrary.org/obo/CHEBI_74698"},{"id":"A54","pred":"chebi_id","subj":"T54","obj":"http://purl.obolibrary.org/obo/CHEBI_141439"},{"id":"A55","pred":"chebi_id","subj":"T55","obj":"http://purl.obolibrary.org/obo/CHEBI_25367"},{"id":"A56","pred":"chebi_id","subj":"T56","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID: 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1). Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B). The monomers shared a similar structural core preceded by a flexible region (Figure S1D). The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent. Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric."}

{"project":"LitCovid-sentences","denotations":[{"id":"T32","span":{"begin":0,"end":131},"obj":"Sentence"},{"id":"T33","span":{"begin":132,"end":291},"obj":"Sentence"},{"id":"T34","span":{"begin":292,"end":445},"obj":"Sentence"},{"id":"T35","span":{"begin":446,"end":535},"obj":"Sentence"},{"id":"T36","span":{"begin":536,"end":827},"obj":"Sentence"},{"id":"T37","span":{"begin":828,"end":932},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID: 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1). Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B). The monomers shared a similar structural core preceded by a flexible region (Figure S1D). The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent. Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric."}

{"project":"LitCovid-PubTator","denotations":[{"id":"93","span":{"begin":39,"end":47},"obj":"Species"},{"id":"94","span":{"begin":107,"end":116},"obj":"Species"},{"id":"95","span":{"begin":690,"end":694},"obj":"Species"},{"id":"96","span":{"begin":801,"end":804},"obj":"Species"},{"id":"97","span":{"begin":328,"end":333},"obj":"Chemical"}],"attributes":[{"id":"A93","pred":"tao:has_database_id","subj":"93","obj":"Tax:1335626"},{"id":"A94","pred":"tao:has_database_id","subj":"94","obj":"Tax:31631"},{"id":"A95","pred":"tao:has_database_id","subj":"95","obj":"Tax:11118"},{"id":"A96","pred":"tao:has_database_id","subj":"96","obj":"Tax:11118"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID: 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1). Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B). The monomers shared a similar structural core preceded by a flexible region (Figure S1D). The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent. Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric."}

{"project":"2_test","denotations":[{"id":"32105468-16338414-61929504","span":{"begin":695,"end":697},"obj":"16338414"}],"text":"We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID: 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1). Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B). The monomers shared a similar structural core preceded by a flexible region (Figure S1D). The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent. Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric."}